Single-cell sequencing reveals cellular differences and potential mechanisms in congenital pulmonary airway malformation
ObjectivesCongenital pulmonary airway malformation (CPAM) is a common fetal lung developmental abnormality whose pathological process is not fully understood.MethodSingle-cell sequencing technology is a novel high-throughput method that can reveal differences between different cell types and their r...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2025.1548177/full |
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| author | Jinxi Huang Jinxi Huang Qiang Chen Qiang Chen Songming Hong Songming Hong Junjie Hong Junjie Hong Hua Cao |
| author_facet | Jinxi Huang Jinxi Huang Qiang Chen Qiang Chen Songming Hong Songming Hong Junjie Hong Junjie Hong Hua Cao |
| author_sort | Jinxi Huang |
| collection | DOAJ |
| description | ObjectivesCongenital pulmonary airway malformation (CPAM) is a common fetal lung developmental abnormality whose pathological process is not fully understood.MethodSingle-cell sequencing technology is a novel high-throughput method that can reveal differences between different cell types and their role in disease.ResultsBy analyzing single-cell sequencing data from CPAM lesion tissue and normal tissue, we found an increase in erythrocytes, plasma cells and mast cells in CPAM samples. Additionally, genes such as CCL5, NKG7, GZMB, and SCGB1A1 were highly expressed in CPAM lesion tissues. The differentially expressed genes in CPAM samples are mainly related to functions such as inflammatory response, tissue remodeling, and immune response. Moreover, analysis of the signaling pathways involved in these differentially expressed genes revealed that pathways such as lysosome, phagosome, adherens junction, focal adhesion, and protein processing in the endoplasmic reticulum may be associated with the pathological process of CPAM.ConclusionThis study provides an in-depth analysis of the cellular differences between CPAM tissues and normal tissues using single-cell sequencing technology, revealing key cell types and functions involved in the development of CPAM. These findings provide important clues for a better understanding of the pathological process of CPAM and serve as a basis for identifying potential therapeutic targets. |
| format | Article |
| id | doaj-art-1a062d4d811d44cd8fe1b9d2a32b8e20 |
| institution | DOAJ |
| issn | 2296-858X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Medicine |
| spelling | doaj-art-1a062d4d811d44cd8fe1b9d2a32b8e202025-08-20T03:19:24ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2025-06-011210.3389/fmed.2025.15481771548177Single-cell sequencing reveals cellular differences and potential mechanisms in congenital pulmonary airway malformationJinxi Huang0Jinxi Huang1Qiang Chen2Qiang Chen3Songming Hong4Songming Hong5Junjie Hong6Junjie Hong7Hua Cao8Department of Cardiothoracic Surgery, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, ChinaFujian Children’s Hospital (Fujian Branch of Shanghai Children’s Medical Center), Fuzhou, ChinaDepartment of Cardiothoracic Surgery, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, ChinaFujian Children’s Hospital (Fujian Branch of Shanghai Children’s Medical Center), Fuzhou, ChinaDepartment of Cardiothoracic Surgery, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, ChinaFujian Children’s Hospital (Fujian Branch of Shanghai Children’s Medical Center), Fuzhou, ChinaDepartment of Cardiothoracic Surgery, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, ChinaFujian Children’s Hospital (Fujian Branch of Shanghai Children’s Medical Center), Fuzhou, ChinaDepartment of Cardiothoracic Surgery, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, ChinaObjectivesCongenital pulmonary airway malformation (CPAM) is a common fetal lung developmental abnormality whose pathological process is not fully understood.MethodSingle-cell sequencing technology is a novel high-throughput method that can reveal differences between different cell types and their role in disease.ResultsBy analyzing single-cell sequencing data from CPAM lesion tissue and normal tissue, we found an increase in erythrocytes, plasma cells and mast cells in CPAM samples. Additionally, genes such as CCL5, NKG7, GZMB, and SCGB1A1 were highly expressed in CPAM lesion tissues. The differentially expressed genes in CPAM samples are mainly related to functions such as inflammatory response, tissue remodeling, and immune response. Moreover, analysis of the signaling pathways involved in these differentially expressed genes revealed that pathways such as lysosome, phagosome, adherens junction, focal adhesion, and protein processing in the endoplasmic reticulum may be associated with the pathological process of CPAM.ConclusionThis study provides an in-depth analysis of the cellular differences between CPAM tissues and normal tissues using single-cell sequencing technology, revealing key cell types and functions involved in the development of CPAM. These findings provide important clues for a better understanding of the pathological process of CPAM and serve as a basis for identifying potential therapeutic targets.https://www.frontiersin.org/articles/10.3389/fmed.2025.1548177/fullCPAMsingle-cell sequencingKEGGinflammation responseDEGs |
| spellingShingle | Jinxi Huang Jinxi Huang Qiang Chen Qiang Chen Songming Hong Songming Hong Junjie Hong Junjie Hong Hua Cao Single-cell sequencing reveals cellular differences and potential mechanisms in congenital pulmonary airway malformation Frontiers in Medicine CPAM single-cell sequencing KEGG inflammation response DEGs |
| title | Single-cell sequencing reveals cellular differences and potential mechanisms in congenital pulmonary airway malformation |
| title_full | Single-cell sequencing reveals cellular differences and potential mechanisms in congenital pulmonary airway malformation |
| title_fullStr | Single-cell sequencing reveals cellular differences and potential mechanisms in congenital pulmonary airway malformation |
| title_full_unstemmed | Single-cell sequencing reveals cellular differences and potential mechanisms in congenital pulmonary airway malformation |
| title_short | Single-cell sequencing reveals cellular differences and potential mechanisms in congenital pulmonary airway malformation |
| title_sort | single cell sequencing reveals cellular differences and potential mechanisms in congenital pulmonary airway malformation |
| topic | CPAM single-cell sequencing KEGG inflammation response DEGs |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2025.1548177/full |
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