Biopreservation and reversal of oxidative injury during blood storage by a novel curcumin-based gel formulation
Abstract Blood storage lesion induces cytosolic and membrane changes driven in part by hemoglobin (Hb) oxidation reactions within red blood cells (RBCs). A novel gel formulation containing the antioxidant curcuminoids in a biocompatible solvent system was used to deliver curcumin into RBCs. Incubati...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-12-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-82943-1 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846101314053865472 |
|---|---|
| author | Wayne Hicks Sirsendu Jana Tigist Kassa Richard Prince Pedro Cabrales Joel Friedman Abdu I. Alayash |
| author_facet | Wayne Hicks Sirsendu Jana Tigist Kassa Richard Prince Pedro Cabrales Joel Friedman Abdu I. Alayash |
| author_sort | Wayne Hicks |
| collection | DOAJ |
| description | Abstract Blood storage lesion induces cytosolic and membrane changes driven in part by hemoglobin (Hb) oxidation reactions within red blood cells (RBCs). A novel gel formulation containing the antioxidant curcuminoids in a biocompatible solvent system was used to deliver curcumin into RBCs. Incubation of peroxide treated RBCs stored in PBS with curcumin gel led to a reduction in prooxidant ferrylHb and recovery in ATP. Curcumin treatment prevented band 3 tyrosine (Y359 and Y21) phosphorylation. RBCs stored in AS-3 solutions for 28, 35, 42 and 49 days, following a single-dose of 100µM curcuminoids at each time points, caused reduction in protein carbonylation and considerable recovery in ATP levels. Proteomic analysis revealed minimal changes in the proteomic landscape in 35 days. However, a downregulation in fibrinogen was observed in the treated samples which may reduce RBC aggregation. Additionally, we used a guinea pig model where the circulation of infused aged RBCs can be extended (approximately 10%) when treated with curcumin gel at the start of storage. Our data therefore provide mechanistic insights and supportive animal data into benefits of treating stored RBCs with a novel curcuminoid formulation based on the biopreservation of RBC membrane integrity, redox balance, and increased longevity in circulation. |
| format | Article |
| id | doaj-art-19e41a33ccb14999b4620e094901a00b |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-19e41a33ccb14999b4620e094901a00b2024-12-29T12:17:07ZengNature PortfolioScientific Reports2045-23222024-12-0114111510.1038/s41598-024-82943-1Biopreservation and reversal of oxidative injury during blood storage by a novel curcumin-based gel formulationWayne Hicks0Sirsendu Jana1Tigist Kassa2Richard Prince3Pedro Cabrales4Joel Friedman5Abdu I. Alayash6Laboratory of Biochemistry and Vascular Biology, Center for Biologic Evaluation and Research, Food and Drug AdministrationLaboratory of Biochemistry and Vascular Biology, Center for Biologic Evaluation and Research, Food and Drug AdministrationLaboratory of Biochemistry and Vascular Biology, Center for Biologic Evaluation and Research, Food and Drug AdministrationVascarta IncUniversity of CaliforniaVascarta IncLaboratory of Biochemistry and Vascular Biology, Center for Biologic Evaluation and Research, Food and Drug AdministrationAbstract Blood storage lesion induces cytosolic and membrane changes driven in part by hemoglobin (Hb) oxidation reactions within red blood cells (RBCs). A novel gel formulation containing the antioxidant curcuminoids in a biocompatible solvent system was used to deliver curcumin into RBCs. Incubation of peroxide treated RBCs stored in PBS with curcumin gel led to a reduction in prooxidant ferrylHb and recovery in ATP. Curcumin treatment prevented band 3 tyrosine (Y359 and Y21) phosphorylation. RBCs stored in AS-3 solutions for 28, 35, 42 and 49 days, following a single-dose of 100µM curcuminoids at each time points, caused reduction in protein carbonylation and considerable recovery in ATP levels. Proteomic analysis revealed minimal changes in the proteomic landscape in 35 days. However, a downregulation in fibrinogen was observed in the treated samples which may reduce RBC aggregation. Additionally, we used a guinea pig model where the circulation of infused aged RBCs can be extended (approximately 10%) when treated with curcumin gel at the start of storage. Our data therefore provide mechanistic insights and supportive animal data into benefits of treating stored RBCs with a novel curcuminoid formulation based on the biopreservation of RBC membrane integrity, redox balance, and increased longevity in circulation.https://doi.org/10.1038/s41598-024-82943-1CurcuminVAS-101Red blood cellsFibrinogen |
| spellingShingle | Wayne Hicks Sirsendu Jana Tigist Kassa Richard Prince Pedro Cabrales Joel Friedman Abdu I. Alayash Biopreservation and reversal of oxidative injury during blood storage by a novel curcumin-based gel formulation Scientific Reports Curcumin VAS-101 Red blood cells Fibrinogen |
| title | Biopreservation and reversal of oxidative injury during blood storage by a novel curcumin-based gel formulation |
| title_full | Biopreservation and reversal of oxidative injury during blood storage by a novel curcumin-based gel formulation |
| title_fullStr | Biopreservation and reversal of oxidative injury during blood storage by a novel curcumin-based gel formulation |
| title_full_unstemmed | Biopreservation and reversal of oxidative injury during blood storage by a novel curcumin-based gel formulation |
| title_short | Biopreservation and reversal of oxidative injury during blood storage by a novel curcumin-based gel formulation |
| title_sort | biopreservation and reversal of oxidative injury during blood storage by a novel curcumin based gel formulation |
| topic | Curcumin VAS-101 Red blood cells Fibrinogen |
| url | https://doi.org/10.1038/s41598-024-82943-1 |
| work_keys_str_mv | AT waynehicks biopreservationandreversalofoxidativeinjuryduringbloodstoragebyanovelcurcuminbasedgelformulation AT sirsendujana biopreservationandreversalofoxidativeinjuryduringbloodstoragebyanovelcurcuminbasedgelformulation AT tigistkassa biopreservationandreversalofoxidativeinjuryduringbloodstoragebyanovelcurcuminbasedgelformulation AT richardprince biopreservationandreversalofoxidativeinjuryduringbloodstoragebyanovelcurcuminbasedgelformulation AT pedrocabrales biopreservationandreversalofoxidativeinjuryduringbloodstoragebyanovelcurcuminbasedgelformulation AT joelfriedman biopreservationandreversalofoxidativeinjuryduringbloodstoragebyanovelcurcuminbasedgelformulation AT abduialayash biopreservationandreversalofoxidativeinjuryduringbloodstoragebyanovelcurcuminbasedgelformulation |