Spreading of antibiotic resistance as a result of soil fertilization by manure composts containing oxytetracycline and antibiotic-resistant genes
Due to the active usage of antibacterial drugs in animal husbandry, antibiotic residues and antibiotic-resistance genes enter the soil when it is fertilized with manure and compost. In this work, we estimated the spread of antibiotic-resistance genes in the soil when it was fertilized with composts...
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| Format: | Article |
| Language: | English |
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Kazan Federal University
2019-09-01
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| Series: | Учёные записки Казанского университета: Серия Естественные науки |
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| Online Access: | https://kpfu.ru/uz-eng-ns-2019-3-4.html |
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| author | N.V. Danilova P.A. Kuryntseva M.Sh. Tagirov P.Yu. Galitskaya S.Yu. Selivanovskaya |
| author_facet | N.V. Danilova P.A. Kuryntseva M.Sh. Tagirov P.Yu. Galitskaya S.Yu. Selivanovskaya |
| author_sort | N.V. Danilova |
| collection | DOAJ |
| description | Due to the active usage of antibacterial drugs in animal husbandry, antibiotic residues and antibiotic-resistance genes enter the soil when it is fertilized with manure and compost. In this work, we estimated the spread of antibiotic-resistance genes in the soil when it was fertilized with composts containing oxytetracycline (OTC) and tetracycline-resistance genes. The following mixtures were studied for 35 days: the soil with “clean” compost, the soil with compost and 300 mg kg–1 OTC, the soil with compost and antibiotic-resistance genes. In the soil treated with composts, the number of bacteria and fungi increased, with the greatest changes in the number of bacteria observed for the soil with compost containing OTC. The addition of compost with the antibiotic and the resistance genes tet(M) and tet(X) to the soil led to the formation of antibiotic resistance in soil microorganisms with the highest number of gene copies for 5–7 days: an average of 3.31?106 and 7.77?106 copies g-1, respectively. Both genes, tet(M) and tet(X), but in smaller numbers, were detected in the soil with “clean” compost on days 14–21, respectively. |
| format | Article |
| id | doaj-art-19bd004440424b4f9fbe01d8b7aefbb2 |
| institution | Kabale University |
| issn | 2542-064X 2500-218X |
| language | English |
| publishDate | 2019-09-01 |
| publisher | Kazan Federal University |
| record_format | Article |
| series | Учёные записки Казанского университета: Серия Естественные науки |
| spelling | doaj-art-19bd004440424b4f9fbe01d8b7aefbb22025-01-02T11:36:19ZengKazan Federal UniversityУчёные записки Казанского университета: Серия Естественные науки2542-064X2500-218X2019-09-01161339540710.26907/2542-064X.2019.3.395-407Spreading of antibiotic resistance as a result of soil fertilization by manure composts containing oxytetracycline and antibiotic-resistant genesN.V. Danilova0P.A. Kuryntseva1M.Sh. Tagirov2P.Yu. Galitskaya3S.Yu. Selivanovskaya4Kazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaTatar Scientific Research Institute of Agriculture, FRC Kazan Scientific Center, Russian Academy of Sciences, Kazan, 420059 RussiaKazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaDue to the active usage of antibacterial drugs in animal husbandry, antibiotic residues and antibiotic-resistance genes enter the soil when it is fertilized with manure and compost. In this work, we estimated the spread of antibiotic-resistance genes in the soil when it was fertilized with composts containing oxytetracycline (OTC) and tetracycline-resistance genes. The following mixtures were studied for 35 days: the soil with “clean” compost, the soil with compost and 300 mg kg–1 OTC, the soil with compost and antibiotic-resistance genes. In the soil treated with composts, the number of bacteria and fungi increased, with the greatest changes in the number of bacteria observed for the soil with compost containing OTC. The addition of compost with the antibiotic and the resistance genes tet(M) and tet(X) to the soil led to the formation of antibiotic resistance in soil microorganisms with the highest number of gene copies for 5–7 days: an average of 3.31?106 and 7.77?106 copies g-1, respectively. Both genes, tet(M) and tet(X), but in smaller numbers, were detected in the soil with “clean” compost on days 14–21, respectively.https://kpfu.ru/uz-eng-ns-2019-3-4.htmlantibioticsoxytetracyclineantibiotic resistanceantibiotic-resistance genescompostreal-time pcr |
| spellingShingle | N.V. Danilova P.A. Kuryntseva M.Sh. Tagirov P.Yu. Galitskaya S.Yu. Selivanovskaya Spreading of antibiotic resistance as a result of soil fertilization by manure composts containing oxytetracycline and antibiotic-resistant genes Учёные записки Казанского университета: Серия Естественные науки antibiotics oxytetracycline antibiotic resistance antibiotic-resistance genes compost real-time pcr |
| title | Spreading of antibiotic resistance as a result of soil fertilization by manure composts containing oxytetracycline and antibiotic-resistant genes |
| title_full | Spreading of antibiotic resistance as a result of soil fertilization by manure composts containing oxytetracycline and antibiotic-resistant genes |
| title_fullStr | Spreading of antibiotic resistance as a result of soil fertilization by manure composts containing oxytetracycline and antibiotic-resistant genes |
| title_full_unstemmed | Spreading of antibiotic resistance as a result of soil fertilization by manure composts containing oxytetracycline and antibiotic-resistant genes |
| title_short | Spreading of antibiotic resistance as a result of soil fertilization by manure composts containing oxytetracycline and antibiotic-resistant genes |
| title_sort | spreading of antibiotic resistance as a result of soil fertilization by manure composts containing oxytetracycline and antibiotic resistant genes |
| topic | antibiotics oxytetracycline antibiotic resistance antibiotic-resistance genes compost real-time pcr |
| url | https://kpfu.ru/uz-eng-ns-2019-3-4.html |
| work_keys_str_mv | AT nvdanilova spreadingofantibioticresistanceasaresultofsoilfertilizationbymanurecompostscontainingoxytetracyclineandantibioticresistantgenes AT pakuryntseva spreadingofantibioticresistanceasaresultofsoilfertilizationbymanurecompostscontainingoxytetracyclineandantibioticresistantgenes AT mshtagirov spreadingofantibioticresistanceasaresultofsoilfertilizationbymanurecompostscontainingoxytetracyclineandantibioticresistantgenes AT pyugalitskaya spreadingofantibioticresistanceasaresultofsoilfertilizationbymanurecompostscontainingoxytetracyclineandantibioticresistantgenes AT syuselivanovskaya spreadingofantibioticresistanceasaresultofsoilfertilizationbymanurecompostscontainingoxytetracyclineandantibioticresistantgenes |