Antibiotic Resistance and Presence of Persister Cells in the Biofilm-like Environments in <i>Streptococcus agalactiae</i>
<b>Objectives:</b> This study investigated antibiotic resistance and presence of persister cells in <i>Streptococcus agalactiae</i> strains belonging to capsular types Ia/ST-103, III/ST-17, and V/ST-26 in biofilm-like environments. <b>Results: </b><i>S. agal...
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| Format: | Article |
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MDPI AG
2024-10-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/13/11/1014 |
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| author | Pamella Silva Lannes-Costa Isabelle Rodrigues Fernandes João Matheus Sobral Pena Brunno Renato Farias Verçoza Costa Marcel Menezes Lyra da Cunha Bernadete Teixeira Ferreira-Carvalho Prescilla Emy Nagao |
| author_facet | Pamella Silva Lannes-Costa Isabelle Rodrigues Fernandes João Matheus Sobral Pena Brunno Renato Farias Verçoza Costa Marcel Menezes Lyra da Cunha Bernadete Teixeira Ferreira-Carvalho Prescilla Emy Nagao |
| author_sort | Pamella Silva Lannes-Costa |
| collection | DOAJ |
| description | <b>Objectives:</b> This study investigated antibiotic resistance and presence of persister cells in <i>Streptococcus agalactiae</i> strains belonging to capsular types Ia/ST-103, III/ST-17, and V/ST-26 in biofilm-like environments. <b>Results: </b><i>S. agalactiae</i> strains were susceptible to penicillin, clindamycin, and erythromycin. Resistance genes were associated with <i>tet</i>M (80%), <i>tet</i>O (20%), <i>erm</i>B (80%), and <i>lin</i>B (40%). Persister cells were detected in bacterial strains exposed to high concentrations of penicillin, clindamycin, and erythromycin. <i>S. agalactiae</i> capsular type III/ST-17 exhibited the highest percentage of persister cells in response to penicillin and clindamycin, while type Ia/ST-103 presented the lowest percentages of persister cells for all antimicrobials tested. Additionally, persister cells were also detected at lower levels for erythromycin, regardless of capsular type or sequence type. Further, all <i>S. agalactiae</i> isolates presented efflux pump activity in ethidium bromide-refractory cell assays. LIVE/DEAD fluorescence microscopy confirmed the presence of >85% viable persister cells after antibiotic treatment. <b>Conclusions:</b> These findings suggest that persister cells play a key role in the persistence of <i>S. agalactiae</i> during antibiotic therapy, interfering with the treatment of invasive infections. Monitoring persister formation is crucial for developing strategies to combat recurrent infections caused by this pathogen. |
| format | Article |
| id | doaj-art-19b27cec773e41f0ba80b448701f944e |
| institution | Kabale University |
| issn | 2079-6382 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj-art-19b27cec773e41f0ba80b448701f944e2024-11-26T17:46:22ZengMDPI AGAntibiotics2079-63822024-10-011311101410.3390/antibiotics13111014Antibiotic Resistance and Presence of Persister Cells in the Biofilm-like Environments in <i>Streptococcus agalactiae</i>Pamella Silva Lannes-Costa0Isabelle Rodrigues Fernandes1João Matheus Sobral Pena2Brunno Renato Farias Verçoza Costa3Marcel Menezes Lyra da Cunha4Bernadete Teixeira Ferreira-Carvalho5Prescilla Emy Nagao6Laboratory of Molecular Biology and Physiology of Streptococci, Institute of Biology Roberto Alcantara Gomes, Rio de Janeiro State University—UERJ, Rio de Janeiro 20550-013, RJ, BrazilLaboratory of Molecular Biology and Physiology of Streptococci, Institute of Biology Roberto Alcantara Gomes, Rio de Janeiro State University—UERJ, Rio de Janeiro 20550-013, RJ, BrazilLaboratory of Molecular Biology and Physiology of Streptococci, Institute of Biology Roberto Alcantara Gomes, Rio de Janeiro State University—UERJ, Rio de Janeiro 20550-013, RJ, BrazilNúcleo Multidisciplinar de Pesquisa UFRJ—Xerém em Biologia, Campus UFRJ—Duque de Caxias Professor Geraldo Cidade, Universidade Federal do Rio de Janeiro, Rio de Janeiro 25240-005, RJ, BrazilNúcleo Multidisciplinar de Pesquisa UFRJ—Xerém em Biologia, Campus UFRJ—Duque de Caxias Professor Geraldo Cidade, Universidade Federal do Rio de Janeiro, Rio de Janeiro 25240-005, RJ, BrazilDepartamento de Microbiologia Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, BrazilLaboratory of Molecular Biology and Physiology of Streptococci, Institute of Biology Roberto Alcantara Gomes, Rio de Janeiro State University—UERJ, Rio de Janeiro 20550-013, RJ, Brazil<b>Objectives:</b> This study investigated antibiotic resistance and presence of persister cells in <i>Streptococcus agalactiae</i> strains belonging to capsular types Ia/ST-103, III/ST-17, and V/ST-26 in biofilm-like environments. <b>Results: </b><i>S. agalactiae</i> strains were susceptible to penicillin, clindamycin, and erythromycin. Resistance genes were associated with <i>tet</i>M (80%), <i>tet</i>O (20%), <i>erm</i>B (80%), and <i>lin</i>B (40%). Persister cells were detected in bacterial strains exposed to high concentrations of penicillin, clindamycin, and erythromycin. <i>S. agalactiae</i> capsular type III/ST-17 exhibited the highest percentage of persister cells in response to penicillin and clindamycin, while type Ia/ST-103 presented the lowest percentages of persister cells for all antimicrobials tested. Additionally, persister cells were also detected at lower levels for erythromycin, regardless of capsular type or sequence type. Further, all <i>S. agalactiae</i> isolates presented efflux pump activity in ethidium bromide-refractory cell assays. LIVE/DEAD fluorescence microscopy confirmed the presence of >85% viable persister cells after antibiotic treatment. <b>Conclusions:</b> These findings suggest that persister cells play a key role in the persistence of <i>S. agalactiae</i> during antibiotic therapy, interfering with the treatment of invasive infections. Monitoring persister formation is crucial for developing strategies to combat recurrent infections caused by this pathogen.https://www.mdpi.com/2079-6382/13/11/1014<i>Streptococcus agalactiae</i>persisterantibiotic resistance |
| spellingShingle | Pamella Silva Lannes-Costa Isabelle Rodrigues Fernandes João Matheus Sobral Pena Brunno Renato Farias Verçoza Costa Marcel Menezes Lyra da Cunha Bernadete Teixeira Ferreira-Carvalho Prescilla Emy Nagao Antibiotic Resistance and Presence of Persister Cells in the Biofilm-like Environments in <i>Streptococcus agalactiae</i> Antibiotics <i>Streptococcus agalactiae</i> persister antibiotic resistance |
| title | Antibiotic Resistance and Presence of Persister Cells in the Biofilm-like Environments in <i>Streptococcus agalactiae</i> |
| title_full | Antibiotic Resistance and Presence of Persister Cells in the Biofilm-like Environments in <i>Streptococcus agalactiae</i> |
| title_fullStr | Antibiotic Resistance and Presence of Persister Cells in the Biofilm-like Environments in <i>Streptococcus agalactiae</i> |
| title_full_unstemmed | Antibiotic Resistance and Presence of Persister Cells in the Biofilm-like Environments in <i>Streptococcus agalactiae</i> |
| title_short | Antibiotic Resistance and Presence of Persister Cells in the Biofilm-like Environments in <i>Streptococcus agalactiae</i> |
| title_sort | antibiotic resistance and presence of persister cells in the biofilm like environments in i streptococcus agalactiae i |
| topic | <i>Streptococcus agalactiae</i> persister antibiotic resistance |
| url | https://www.mdpi.com/2079-6382/13/11/1014 |
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