In-vitro effect of the methanolic extract of Morinda citrifolia against the life cycle of Dermatobia hominis
Background: bovine cutaneous dermatobiosis or furuncular myiasis caused by Dermatobia hominis is a parasitosis that mainly affects bovines in the tropics and represents a particular interest in public health as zoonosis. Its control is based on ivermectins, which have long withdrawal times, affectin...
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Universidad de Antioquia
2024-12-01
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| Series: | Vitae |
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| Online Access: | https://revistas.udea.edu.co/index.php/vitae/article/view/353998 |
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| author | Dumar Alexander Jaramillo Hernández Rafael Felipe Quevedo Carrillo Diego Arnaldo Cadena Franco Angélica Elizabeth Gonzalez Reina Lida Carolina Lesmes-Rodríguez Luz Natalia Pedraza-Castillo |
| author_facet | Dumar Alexander Jaramillo Hernández Rafael Felipe Quevedo Carrillo Diego Arnaldo Cadena Franco Angélica Elizabeth Gonzalez Reina Lida Carolina Lesmes-Rodríguez Luz Natalia Pedraza-Castillo |
| author_sort | Dumar Alexander Jaramillo Hernández |
| collection | DOAJ |
| description | Background: bovine cutaneous dermatobiosis or furuncular myiasis caused by Dermatobia hominis is a parasitosis that mainly affects bovines in the tropics and represents a particular interest in public health as zoonosis. Its control is based on ivermectins, which have long withdrawal times, affecting the productive dynamics within dairy cattle herds.
Objective: to assess the in-vitro effect of the methanolic extract of the M. citrifolia ripe fruit against the life cycle of D. hominis.
Methods: D. hominis larvae were taken directly from naturally parasitized bovine skins. These larvae were exposed by immersion to different concentrations of the methanolic extract of M. citrifolia (10, 50, 100, 200, 300, 400, 460 mg/mL) diluted in distilled water. Ivermectin 1% was used as a positive control, and distilled water as a negative control. Subsequently, the larvicidal activity was evaluated in the first 48 hours post-immersion (PI), the pupicidal activity within 10 to 23 days PI, and the inhibition of the imagos emergence as well as their anatomical alterations, were evaluated within 24 to 35 days PI; recreating the pupal development and their hatching in the soil under controlled laboratory conditions. CL50 and CL90 for the larvae phase were estimated through Probit regression analysis.
Results: M. citrofolia concentrations of 400 and 460 mg/mL had a significant (p<0.05) larvicidal effect of 40% (95% CI 34.7 - 43.9) and 60% (95% CI 56.8 - 67.3), respectively. The pupicidal effect on the surviving larvae was significant (p<0.05) at 300, 400, and 460 mg/mL: 40% (95% CI 37.9 - 42.3), 60% (95% CI 55.7 - 65.9) and 70% (CI 95% 67.1 – 76.7), respectively. The inhibition of the emergence of imagos was significant (p<0.05) 50% (95% CI 42.3 - 57.8) in all concentrations equal to or greater than 200 mg/mL. Finally, 20% (95% CI 12.6 - 29.3) of the emerging imagos at 460 mg/mL presented morphoananomic alterations (p<0.05). The LC50 and LC90 estimated (larval phase) were 22.36 mg/mL (95%CI 15.06-33.19) and 245.08 mg/mL (95%CI 165.10-363.82), respectively.
Conclusions: The methanolic extract of M. citrifolia was effective as larvicide, altering the pupation and the emergence of imagos of D. hominis. In addition, it modified the imagos morphoanatomy; interesting results to promote in-situ and other bioguided fractionation studies of this extract in different D. hominis stages; being M. citrifolia a plant species widely adapted to the conditions of the Meta department, Colombia.
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| format | Article |
| id | doaj-art-1950ac9c169943a48b4f4fd667c9b72f |
| institution | Kabale University |
| issn | 0121-4004 2145-2660 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Universidad de Antioquia |
| record_format | Article |
| series | Vitae |
| spelling | doaj-art-1950ac9c169943a48b4f4fd667c9b72f2025-01-17T03:51:25ZengUniversidad de AntioquiaVitae0121-40042145-26602024-12-0131310.17533/udea.vitae.v31n3a353998In-vitro effect of the methanolic extract of Morinda citrifolia against the life cycle of Dermatobia hominisDumar Alexander Jaramillo Hernández0Rafael Felipe Quevedo Carrillo1Diego Arnaldo Cadena Franco2Angélica Elizabeth Gonzalez Reina3Lida Carolina Lesmes-Rodríguez4Luz Natalia Pedraza-Castillo5Professor Universidad de los LlanosFacultad de Ciencias Humanas y de la Educación, Universidad de los LlanosFacultad de Ciencias Humanas y de la Educación, Universidad de los LlanosFacultad de Ciencias Agropecuarias y Recursos Naturales, Escuela de Ciencias Animales, Universidad de los LlanosFacultad de Ciencias Básicas e Ingeniería, Departamento de Biología & Química, Universidad de los LlanosFacultad de Ciencias Agropecuarias y Recursos Naturales, Escuela de Ciencias Animales, Universidad de los LlanosBackground: bovine cutaneous dermatobiosis or furuncular myiasis caused by Dermatobia hominis is a parasitosis that mainly affects bovines in the tropics and represents a particular interest in public health as zoonosis. Its control is based on ivermectins, which have long withdrawal times, affecting the productive dynamics within dairy cattle herds. Objective: to assess the in-vitro effect of the methanolic extract of the M. citrifolia ripe fruit against the life cycle of D. hominis. Methods: D. hominis larvae were taken directly from naturally parasitized bovine skins. These larvae were exposed by immersion to different concentrations of the methanolic extract of M. citrifolia (10, 50, 100, 200, 300, 400, 460 mg/mL) diluted in distilled water. Ivermectin 1% was used as a positive control, and distilled water as a negative control. Subsequently, the larvicidal activity was evaluated in the first 48 hours post-immersion (PI), the pupicidal activity within 10 to 23 days PI, and the inhibition of the imagos emergence as well as their anatomical alterations, were evaluated within 24 to 35 days PI; recreating the pupal development and their hatching in the soil under controlled laboratory conditions. CL50 and CL90 for the larvae phase were estimated through Probit regression analysis. Results: M. citrofolia concentrations of 400 and 460 mg/mL had a significant (p<0.05) larvicidal effect of 40% (95% CI 34.7 - 43.9) and 60% (95% CI 56.8 - 67.3), respectively. The pupicidal effect on the surviving larvae was significant (p<0.05) at 300, 400, and 460 mg/mL: 40% (95% CI 37.9 - 42.3), 60% (95% CI 55.7 - 65.9) and 70% (CI 95% 67.1 – 76.7), respectively. The inhibition of the emergence of imagos was significant (p<0.05) 50% (95% CI 42.3 - 57.8) in all concentrations equal to or greater than 200 mg/mL. Finally, 20% (95% CI 12.6 - 29.3) of the emerging imagos at 460 mg/mL presented morphoananomic alterations (p<0.05). The LC50 and LC90 estimated (larval phase) were 22.36 mg/mL (95%CI 15.06-33.19) and 245.08 mg/mL (95%CI 165.10-363.82), respectively. Conclusions: The methanolic extract of M. citrifolia was effective as larvicide, altering the pupation and the emergence of imagos of D. hominis. In addition, it modified the imagos morphoanatomy; interesting results to promote in-situ and other bioguided fractionation studies of this extract in different D. hominis stages; being M. citrifolia a plant species widely adapted to the conditions of the Meta department, Colombia. https://revistas.udea.edu.co/index.php/vitae/article/view/353998cattlemyiasisparasite controlphytotherapy |
| spellingShingle | Dumar Alexander Jaramillo Hernández Rafael Felipe Quevedo Carrillo Diego Arnaldo Cadena Franco Angélica Elizabeth Gonzalez Reina Lida Carolina Lesmes-Rodríguez Luz Natalia Pedraza-Castillo In-vitro effect of the methanolic extract of Morinda citrifolia against the life cycle of Dermatobia hominis Vitae cattle myiasis parasite control phytotherapy |
| title | In-vitro effect of the methanolic extract of Morinda citrifolia against the life cycle of Dermatobia hominis |
| title_full | In-vitro effect of the methanolic extract of Morinda citrifolia against the life cycle of Dermatobia hominis |
| title_fullStr | In-vitro effect of the methanolic extract of Morinda citrifolia against the life cycle of Dermatobia hominis |
| title_full_unstemmed | In-vitro effect of the methanolic extract of Morinda citrifolia against the life cycle of Dermatobia hominis |
| title_short | In-vitro effect of the methanolic extract of Morinda citrifolia against the life cycle of Dermatobia hominis |
| title_sort | in vitro effect of the methanolic extract of morinda citrifolia against the life cycle of dermatobia hominis |
| topic | cattle myiasis parasite control phytotherapy |
| url | https://revistas.udea.edu.co/index.php/vitae/article/view/353998 |
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