Clinical diagnostic performance of droplet digital PCR for pathogen detection in patients with Escherichia coli bloodstream infection: a prospective observational study

Clinical diagnostic performance of droplet digital PCR for pathogen detection in patients with Escherichia coli bloodstream infection: a prospective observational study

Abstract Background Droplet digital PCR (ddPCR) is a highly sensitive tool for detecting bacterial DNA in bacterial bloodstream infections (BSI). This study aimed to examine the sensitivity and specificity of ddPCR and the association between bacterial DNA load in whole blood and the time-to-positiv...

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Main Authors: Hiroki Kitagawa, Masato Kojima, Kayoko Tadera, Shuta Kogasaki, Keitaro Omori, Toshihito Nomura, Norifumi Shigemoto, Eiso Hiyama, Hiroki Ohge
Format: Article
Language:English
Published: BMC 2025-01-01
Series:BMC Infectious Diseases
Subjects:
Droplet digital PCR
Bloodstream infection
Blood culture
Sepsis
Time-to-positivity
Mortality
Online Access:https://doi.org/10.1186/s12879-024-10396-y
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