Novel Drop‐Sampler for Simultaneous Collection of Stereo‐Video, Environmental DNA and Oceanographic Data
ABSTRACT There is an increasing interest in environmental DNA (eDNA) as a method to survey marine biota, enhancing traditional survey methods, and a need to ground truth eDNA‐based interpretations with visual surveys to understand biases in both the eDNA and visual datasets. We designed and tested a...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-12-01
|
| Series: | Ecology and Evolution |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ece3.70705 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846106613315796992 |
|---|---|
| author | Samuel Thompson Simon Jarman Kingsley Griffin Claude Spencer Gabrielle Cummins Julian Partridge Tim Langlois |
| author_facet | Samuel Thompson Simon Jarman Kingsley Griffin Claude Spencer Gabrielle Cummins Julian Partridge Tim Langlois |
| author_sort | Samuel Thompson |
| collection | DOAJ |
| description | ABSTRACT There is an increasing interest in environmental DNA (eDNA) as a method to survey marine biota, enhancing traditional survey methods, and a need to ground truth eDNA‐based interpretations with visual surveys to understand biases in both the eDNA and visual datasets. We designed and tested a rapidly deployable, robust method pairing water sampling for eDNA collection and stereo‐video imagery, comparing inferred fish assemblages with interspersed baited remote underwater video (stereo‐BRUV) samples. The system is capable of rapidly collecting simultaneous wide‐field stereo‐video imagery, oceanographic measurements and multiple water samples across a range of habitats and depths (up to 600 m). A platform demonstration was conducted in a no‐take National Park Zone of the Ningaloo Marine Park, Western Australia, with samples being collected whilst the system is resting on the seafloor. Combining simultaneous visual survey data with eDNA species estimates increased the total diversity of the fish assemblage by ca. 6.5% over eDNA estimates alone, whilst the analysis of the assemblage composition sampled by each method revealed significant differences. The platform demonstration highlights the biases of each sampling method and their complementarity to one another. We suggest that these biases will be better understood by advancements that allow eDNA metabarcoding to discriminate the abundance and life stage of marine biota. Furthermore, investigation of the relationship between eDNA metabarcoding data and concomitant imagery‐derived length, age and habitat data is needed. |
| format | Article |
| id | doaj-art-193d012f7f3145e19d450955b8582cf2 |
| institution | Kabale University |
| issn | 2045-7758 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj-art-193d012f7f3145e19d450955b8582cf22024-12-27T11:24:40ZengWileyEcology and Evolution2045-77582024-12-011412n/an/a10.1002/ece3.70705Novel Drop‐Sampler for Simultaneous Collection of Stereo‐Video, Environmental DNA and Oceanographic DataSamuel Thompson0Simon Jarman1Kingsley Griffin2Claude Spencer3Gabrielle Cummins4Julian Partridge5Tim Langlois6School of Biological Sciences The University of Western Australia Perth WA AustraliaSchool of Biological Sciences The University of Western Australia Perth WA AustraliaSchool of Biological Sciences The University of Western Australia Perth WA AustraliaSchool of Biological Sciences The University of Western Australia Perth WA AustraliaSchool of Biological Sciences The University of Western Australia Perth WA AustraliaSchool of Biological Sciences The University of Western Australia Perth WA AustraliaSchool of Biological Sciences The University of Western Australia Perth WA AustraliaABSTRACT There is an increasing interest in environmental DNA (eDNA) as a method to survey marine biota, enhancing traditional survey methods, and a need to ground truth eDNA‐based interpretations with visual surveys to understand biases in both the eDNA and visual datasets. We designed and tested a rapidly deployable, robust method pairing water sampling for eDNA collection and stereo‐video imagery, comparing inferred fish assemblages with interspersed baited remote underwater video (stereo‐BRUV) samples. The system is capable of rapidly collecting simultaneous wide‐field stereo‐video imagery, oceanographic measurements and multiple water samples across a range of habitats and depths (up to 600 m). A platform demonstration was conducted in a no‐take National Park Zone of the Ningaloo Marine Park, Western Australia, with samples being collected whilst the system is resting on the seafloor. Combining simultaneous visual survey data with eDNA species estimates increased the total diversity of the fish assemblage by ca. 6.5% over eDNA estimates alone, whilst the analysis of the assemblage composition sampled by each method revealed significant differences. The platform demonstration highlights the biases of each sampling method and their complementarity to one another. We suggest that these biases will be better understood by advancements that allow eDNA metabarcoding to discriminate the abundance and life stage of marine biota. Furthermore, investigation of the relationship between eDNA metabarcoding data and concomitant imagery‐derived length, age and habitat data is needed.https://doi.org/10.1002/ece3.70705biodiversityBRUVsdrop‐cameraeDNAfish ecologymetabarcoding |
| spellingShingle | Samuel Thompson Simon Jarman Kingsley Griffin Claude Spencer Gabrielle Cummins Julian Partridge Tim Langlois Novel Drop‐Sampler for Simultaneous Collection of Stereo‐Video, Environmental DNA and Oceanographic Data Ecology and Evolution biodiversity BRUVs drop‐camera eDNA fish ecology metabarcoding |
| title | Novel Drop‐Sampler for Simultaneous Collection of Stereo‐Video, Environmental DNA and Oceanographic Data |
| title_full | Novel Drop‐Sampler for Simultaneous Collection of Stereo‐Video, Environmental DNA and Oceanographic Data |
| title_fullStr | Novel Drop‐Sampler for Simultaneous Collection of Stereo‐Video, Environmental DNA and Oceanographic Data |
| title_full_unstemmed | Novel Drop‐Sampler for Simultaneous Collection of Stereo‐Video, Environmental DNA and Oceanographic Data |
| title_short | Novel Drop‐Sampler for Simultaneous Collection of Stereo‐Video, Environmental DNA and Oceanographic Data |
| title_sort | novel drop sampler for simultaneous collection of stereo video environmental dna and oceanographic data |
| topic | biodiversity BRUVs drop‐camera eDNA fish ecology metabarcoding |
| url | https://doi.org/10.1002/ece3.70705 |
| work_keys_str_mv | AT samuelthompson noveldropsamplerforsimultaneouscollectionofstereovideoenvironmentaldnaandoceanographicdata AT simonjarman noveldropsamplerforsimultaneouscollectionofstereovideoenvironmentaldnaandoceanographicdata AT kingsleygriffin noveldropsamplerforsimultaneouscollectionofstereovideoenvironmentaldnaandoceanographicdata AT claudespencer noveldropsamplerforsimultaneouscollectionofstereovideoenvironmentaldnaandoceanographicdata AT gabriellecummins noveldropsamplerforsimultaneouscollectionofstereovideoenvironmentaldnaandoceanographicdata AT julianpartridge noveldropsamplerforsimultaneouscollectionofstereovideoenvironmentaldnaandoceanographicdata AT timlanglois noveldropsamplerforsimultaneouscollectionofstereovideoenvironmentaldnaandoceanographicdata |