Microbial community analysis of mildewed cigar tobacco leaves from high-throughput sequencing data

Abstract Background Cigar tobacco leaves contain abundant bacteria and fungi that are vital for their quality. In this study, the microbial communities were analyzed in the artificial mildewed cigar tobacco leaves of different mildew stages (healthy control, early stage, middle stage and late stage)...

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Main Authors: Min Wei, Youzhi Shi, Xuyan Song, Lin Rong, Ziwei Li, Jing Li, Bo Wang, Bifeng Chen
Format: Article
Language:English
Published: BMC 2024-11-01
Series:Annals of Microbiology
Subjects:
Online Access:https://doi.org/10.1186/s13213-024-01783-6
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author Min Wei
Youzhi Shi
Xuyan Song
Lin Rong
Ziwei Li
Jing Li
Bo Wang
Bifeng Chen
author_facet Min Wei
Youzhi Shi
Xuyan Song
Lin Rong
Ziwei Li
Jing Li
Bo Wang
Bifeng Chen
author_sort Min Wei
collection DOAJ
description Abstract Background Cigar tobacco leaves contain abundant bacteria and fungi that are vital for their quality. In this study, the microbial communities were analyzed in the artificial mildewed cigar tobacco leaves of different mildew stages (healthy control, early stage, middle stage and late stage). Results For cigar wrapper tobacco leaves, there was an increased bacterial genera abundance of Terribacillus, Bacillus and Micrococcus, while there was an increased fungal genera abundance of Aspergillus, Penicillium and Mucor. For cigar filler tobacco leaves, there was an increased bacterial genera abundance of Staphylococcus, while there was an increased fungal genera abundance of Aspergillus and Trichomonascus. Microbial communities (bacterial and fungal) showed significantly different compositions in both cigar wrapper and filler tobacco leaves from different mildew stages. The top important microbial communities (bacterial and fungal) in cigar wrapper and filler tobacco were Sphingomonas, Aerococcus, Wallemia and Trichomonascus, respectively. Conclusion This study provided evidence for the great changes in microbial communities during the mildew process of cigar wrapper and filler tobacco. The effects of the dominant bacterial genera and fungal genera on tobacco mildew should be explored in depth, whose findings may be applied to develop strategies for controlling tobacco mildew.
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id doaj-art-18dc8f73e7124197b3d3b97f4a497ff2
institution Kabale University
issn 1869-2044
language English
publishDate 2024-11-01
publisher BMC
record_format Article
series Annals of Microbiology
spelling doaj-art-18dc8f73e7124197b3d3b97f4a497ff22024-11-24T12:03:58ZengBMCAnnals of Microbiology1869-20442024-11-0174111110.1186/s13213-024-01783-6Microbial community analysis of mildewed cigar tobacco leaves from high-throughput sequencing dataMin Wei0Youzhi Shi1Xuyan Song2Lin Rong3Ziwei Li4Jing Li5Bo Wang6Bifeng Chen7Technology Centre of Hubei China Tobacco Industry Co. LtdTechnology Centre of Hubei China Tobacco Industry Co. LtdTechnology Centre of Hubei China Tobacco Industry Co. LtdTechnology Centre of Hubei China Tobacco Industry Co. LtdTechnology Centre of Hubei China Tobacco Industry Co. LtdSchool of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of TechnologyTechnology Centre of Hubei China Tobacco Industry Co. LtdSchool of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of TechnologyAbstract Background Cigar tobacco leaves contain abundant bacteria and fungi that are vital for their quality. In this study, the microbial communities were analyzed in the artificial mildewed cigar tobacco leaves of different mildew stages (healthy control, early stage, middle stage and late stage). Results For cigar wrapper tobacco leaves, there was an increased bacterial genera abundance of Terribacillus, Bacillus and Micrococcus, while there was an increased fungal genera abundance of Aspergillus, Penicillium and Mucor. For cigar filler tobacco leaves, there was an increased bacterial genera abundance of Staphylococcus, while there was an increased fungal genera abundance of Aspergillus and Trichomonascus. Microbial communities (bacterial and fungal) showed significantly different compositions in both cigar wrapper and filler tobacco leaves from different mildew stages. The top important microbial communities (bacterial and fungal) in cigar wrapper and filler tobacco were Sphingomonas, Aerococcus, Wallemia and Trichomonascus, respectively. Conclusion This study provided evidence for the great changes in microbial communities during the mildew process of cigar wrapper and filler tobacco. The effects of the dominant bacterial genera and fungal genera on tobacco mildew should be explored in depth, whose findings may be applied to develop strategies for controlling tobacco mildew.https://doi.org/10.1186/s13213-024-01783-6Tobacco mildewCigar tobacco leavesBacterial communityFungal community
spellingShingle Min Wei
Youzhi Shi
Xuyan Song
Lin Rong
Ziwei Li
Jing Li
Bo Wang
Bifeng Chen
Microbial community analysis of mildewed cigar tobacco leaves from high-throughput sequencing data
Annals of Microbiology
Tobacco mildew
Cigar tobacco leaves
Bacterial community
Fungal community
title Microbial community analysis of mildewed cigar tobacco leaves from high-throughput sequencing data
title_full Microbial community analysis of mildewed cigar tobacco leaves from high-throughput sequencing data
title_fullStr Microbial community analysis of mildewed cigar tobacco leaves from high-throughput sequencing data
title_full_unstemmed Microbial community analysis of mildewed cigar tobacco leaves from high-throughput sequencing data
title_short Microbial community analysis of mildewed cigar tobacco leaves from high-throughput sequencing data
title_sort microbial community analysis of mildewed cigar tobacco leaves from high throughput sequencing data
topic Tobacco mildew
Cigar tobacco leaves
Bacterial community
Fungal community
url https://doi.org/10.1186/s13213-024-01783-6
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