Application of selection Index for enhancing resistance to Cryptocarya irritans and Vibrio alginolyticus in large yellow croaker
Despite the high cost of genotyping and the limited economic return per individual that restricts the use of genome selection technology in aquatic species breeding, integrating multiple traits in a selection index can reduce costs and present a promising direction for fish breeding. The aim of this...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1524914/full |
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| author | Jiaying Wang Jiaying Wang Ji Zhao Ji Zhao Bo Liu Hongshu Chi Pengxing Jiang Pengxing Jiang Junzu Xiao Junzu Xiao Zhou Jiang Zhou Jiang Lingwei Miao Lingwei Miao Qian He Qian He Junjia Zeng Junjia Zeng Qiaozhen Ke Qiaozhen Ke Tao Zhou Tao Zhou Peng Xu Peng Xu |
| author_facet | Jiaying Wang Jiaying Wang Ji Zhao Ji Zhao Bo Liu Hongshu Chi Pengxing Jiang Pengxing Jiang Junzu Xiao Junzu Xiao Zhou Jiang Zhou Jiang Lingwei Miao Lingwei Miao Qian He Qian He Junjia Zeng Junjia Zeng Qiaozhen Ke Qiaozhen Ke Tao Zhou Tao Zhou Peng Xu Peng Xu |
| author_sort | Jiaying Wang |
| collection | DOAJ |
| description | Despite the high cost of genotyping and the limited economic return per individual that restricts the use of genome selection technology in aquatic species breeding, integrating multiple traits in a selection index can reduce costs and present a promising direction for fish breeding. The aim of this study was to evaluate the feasibility and performance of breeding for dual resistance traits against C. irritans and V. alginolyticus in large yellow croaker, using a selection index approach. A candidate stock population from the C. irritans resistance strain (GS3) of the F2 generation (n = 560) was genotyped using a 55 K SNP array. A total of 50 broodstock with the highest genomic estimated breeding value (GEBV) ranking for the C. irritans resistance trait were selected to produce the F3 generation, while 50 broodstock with the highest selection index were selected to breed the double trait strain (GS9). In parallel, two populations without selective breeding population (C1and C2) were used as the control group to produce the offspring. The genetic improvement effect of all the breeding lines (GS3, GS9, C1, and C2) was assessed through artificial challenge experiments with C. irritans and V. alginolyticus, respectively. The GWAS result indicated that resistance to V. alginolyticus was a polygenic trait and the heritability of resistance to V. alginolyticus was estimated to be approximately 0.1. The survival rates of GS9 and GS3 were 43.74 and 86.81%, respectively, after 120 h of challenge with C. irritans infection, whereas those of C1 and C2 remained at 0%. Furthermore, the survival rates of GS9 and GS3 were 27.03 and 15.48%, respectively, after 48 h of V. alginolyticus infection, while the survival rates of C1 and C2 were 12.67 and 14.17%, respectively. These results indicate that genetic improvement of multiple traits in large yellow croaker using genomic selection technology is indeed feasible. Moreover, this study provides a valuable reference for advancing multi-trait breeding applications in aquatic species. |
| format | Article |
| id | doaj-art-18b94ec39f8541f19d905947f2eb3d58 |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-18b94ec39f8541f19d905947f2eb3d582025-01-17T06:50:56ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-01-011210.3389/fvets.2025.15249141524914Application of selection Index for enhancing resistance to Cryptocarya irritans and Vibrio alginolyticus in large yellow croakerJiaying Wang0Jiaying Wang1Ji Zhao2Ji Zhao3Bo Liu4Hongshu Chi5Pengxing Jiang6Pengxing Jiang7Junzu Xiao8Junzu Xiao9Zhou Jiang10Zhou Jiang11Lingwei Miao12Lingwei Miao13Qian He14Qian He15Junjia Zeng16Junjia Zeng17Qiaozhen Ke18Qiaozhen Ke19Tao Zhou20Tao Zhou21Peng Xu22Peng Xu23State Key Laboratory of Mariculture Breeding, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaFujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaState Key Laboratory of Mariculture Breeding, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaFujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaKey Laboratory of Cultivation and High-Value Utilization of Marine Organisms, Fisheries Research Institute of Fujian, Xiamen, ChinaBiotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, ChinaState Key Laboratory of Mariculture Breeding, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaFujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaState Key Laboratory of Mariculture Breeding, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaFujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaState Key Laboratory of Mariculture Breeding, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaFujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaState Key Laboratory of Mariculture Breeding, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaFujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaState Key Laboratory of Mariculture Breeding, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaFujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaState Key Laboratory of Mariculture Breeding, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaFujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaState Key Laboratory of Mariculture Breeding, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaFujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaState Key Laboratory of Mariculture Breeding, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaFujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaState Key Laboratory of Mariculture Breeding, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaFujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaDespite the high cost of genotyping and the limited economic return per individual that restricts the use of genome selection technology in aquatic species breeding, integrating multiple traits in a selection index can reduce costs and present a promising direction for fish breeding. The aim of this study was to evaluate the feasibility and performance of breeding for dual resistance traits against C. irritans and V. alginolyticus in large yellow croaker, using a selection index approach. A candidate stock population from the C. irritans resistance strain (GS3) of the F2 generation (n = 560) was genotyped using a 55 K SNP array. A total of 50 broodstock with the highest genomic estimated breeding value (GEBV) ranking for the C. irritans resistance trait were selected to produce the F3 generation, while 50 broodstock with the highest selection index were selected to breed the double trait strain (GS9). In parallel, two populations without selective breeding population (C1and C2) were used as the control group to produce the offspring. The genetic improvement effect of all the breeding lines (GS3, GS9, C1, and C2) was assessed through artificial challenge experiments with C. irritans and V. alginolyticus, respectively. The GWAS result indicated that resistance to V. alginolyticus was a polygenic trait and the heritability of resistance to V. alginolyticus was estimated to be approximately 0.1. The survival rates of GS9 and GS3 were 43.74 and 86.81%, respectively, after 120 h of challenge with C. irritans infection, whereas those of C1 and C2 remained at 0%. Furthermore, the survival rates of GS9 and GS3 were 27.03 and 15.48%, respectively, after 48 h of V. alginolyticus infection, while the survival rates of C1 and C2 were 12.67 and 14.17%, respectively. These results indicate that genetic improvement of multiple traits in large yellow croaker using genomic selection technology is indeed feasible. Moreover, this study provides a valuable reference for advancing multi-trait breeding applications in aquatic species.https://www.frontiersin.org/articles/10.3389/fvets.2025.1524914/fullselection indexdisease resistance traitgenomic selectionlarge yellow croakeraquaculture |
| spellingShingle | Jiaying Wang Jiaying Wang Ji Zhao Ji Zhao Bo Liu Hongshu Chi Pengxing Jiang Pengxing Jiang Junzu Xiao Junzu Xiao Zhou Jiang Zhou Jiang Lingwei Miao Lingwei Miao Qian He Qian He Junjia Zeng Junjia Zeng Qiaozhen Ke Qiaozhen Ke Tao Zhou Tao Zhou Peng Xu Peng Xu Application of selection Index for enhancing resistance to Cryptocarya irritans and Vibrio alginolyticus in large yellow croaker Frontiers in Veterinary Science selection index disease resistance trait genomic selection large yellow croaker aquaculture |
| title | Application of selection Index for enhancing resistance to Cryptocarya irritans and Vibrio alginolyticus in large yellow croaker |
| title_full | Application of selection Index for enhancing resistance to Cryptocarya irritans and Vibrio alginolyticus in large yellow croaker |
| title_fullStr | Application of selection Index for enhancing resistance to Cryptocarya irritans and Vibrio alginolyticus in large yellow croaker |
| title_full_unstemmed | Application of selection Index for enhancing resistance to Cryptocarya irritans and Vibrio alginolyticus in large yellow croaker |
| title_short | Application of selection Index for enhancing resistance to Cryptocarya irritans and Vibrio alginolyticus in large yellow croaker |
| title_sort | application of selection index for enhancing resistance to cryptocarya irritans and vibrio alginolyticus in large yellow croaker |
| topic | selection index disease resistance trait genomic selection large yellow croaker aquaculture |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1524914/full |
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