MicroRNA Regulation for Inflammasomes in High Glucose-Treated ARPE-19 Cells
Purpose. This study aimed to evaluate the expression of microRNAs (miRNAs) and inflammasomes in diabetes-induced retinal cells and to determine their role in the pathogenesis of diabetic retinopathy (DR). Methods. To establish diabetes-induced cell models, ARPE-19 cells were treated with high glucos...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2024/3654690 |
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| _version_ | 1841561389709131776 |
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| author | Ji Hong Kim Hyoseon Yu Ji Hye Kang Eun Hee Hong Min Ho Kang Mincheol Seong Heeyoon Cho Yong Un Shin |
| author_facet | Ji Hong Kim Hyoseon Yu Ji Hye Kang Eun Hee Hong Min Ho Kang Mincheol Seong Heeyoon Cho Yong Un Shin |
| author_sort | Ji Hong Kim |
| collection | DOAJ |
| description | Purpose. This study aimed to evaluate the expression of microRNAs (miRNAs) and inflammasomes in diabetes-induced retinal cells and to determine their role in the pathogenesis of diabetic retinopathy (DR). Methods. To establish diabetes-induced cell models, ARPE-19 cells were treated with high glucose. The expression levels of five miRNAs (miR-185, miR-17, miR-20a, miR-15a, and miR-15b) were measured in high glucose-treated ARPE-19 cells using real-time quantitative polymerase chain reaction. Western blotting was performed to measure inflammasome expression in cellular models. miR-17 was selected as the target miRNA, and inflammasome expression was measured following the transfection of an miR-17 mimic into high glucose-treated ARPE-19 cells. Results. In high glucose-treated ARPE-19 cells, miRNA expression was substantially downregulated, whereas that of inflammasome components was significantly increased. Following the transfection of the miR-17 mimic into high glucose-treated ARPE-19 cells, the levels of inflammasome components were significantly decreased. Conclusions. This study investigated the relationship between miRNAs and inflammasomes in diabetes-induced cells using high glucose-treated ARPE-19 cells. These findings suggested that miR-17 suppresses inflammasomes, thereby reducing the subsequent inflammatory response and indicating that miRNAs and inflammasomes could serve as new therapeutic targets for DR. |
| format | Article |
| id | doaj-art-1843d005e11946f28334b634318c34e3 |
| institution | Kabale University |
| issn | 2090-0058 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-1843d005e11946f28334b634318c34e32025-01-03T01:41:34ZengWileyJournal of Ophthalmology2090-00582024-01-01202410.1155/2024/3654690MicroRNA Regulation for Inflammasomes in High Glucose-Treated ARPE-19 CellsJi Hong Kim0Hyoseon Yu1Ji Hye Kang2Eun Hee Hong3Min Ho Kang4Mincheol Seong5Heeyoon Cho6Yong Un Shin7Department of OphthalmologyDepartment of OphthalmologyDepartment of OphthalmologyDepartment of OphthalmologyDepartment of OphthalmologyDepartment of OphthalmologyDepartment of OphthalmologyDepartment of OphthalmologyPurpose. This study aimed to evaluate the expression of microRNAs (miRNAs) and inflammasomes in diabetes-induced retinal cells and to determine their role in the pathogenesis of diabetic retinopathy (DR). Methods. To establish diabetes-induced cell models, ARPE-19 cells were treated with high glucose. The expression levels of five miRNAs (miR-185, miR-17, miR-20a, miR-15a, and miR-15b) were measured in high glucose-treated ARPE-19 cells using real-time quantitative polymerase chain reaction. Western blotting was performed to measure inflammasome expression in cellular models. miR-17 was selected as the target miRNA, and inflammasome expression was measured following the transfection of an miR-17 mimic into high glucose-treated ARPE-19 cells. Results. In high glucose-treated ARPE-19 cells, miRNA expression was substantially downregulated, whereas that of inflammasome components was significantly increased. Following the transfection of the miR-17 mimic into high glucose-treated ARPE-19 cells, the levels of inflammasome components were significantly decreased. Conclusions. This study investigated the relationship between miRNAs and inflammasomes in diabetes-induced cells using high glucose-treated ARPE-19 cells. These findings suggested that miR-17 suppresses inflammasomes, thereby reducing the subsequent inflammatory response and indicating that miRNAs and inflammasomes could serve as new therapeutic targets for DR.http://dx.doi.org/10.1155/2024/3654690 |
| spellingShingle | Ji Hong Kim Hyoseon Yu Ji Hye Kang Eun Hee Hong Min Ho Kang Mincheol Seong Heeyoon Cho Yong Un Shin MicroRNA Regulation for Inflammasomes in High Glucose-Treated ARPE-19 Cells Journal of Ophthalmology |
| title | MicroRNA Regulation for Inflammasomes in High Glucose-Treated ARPE-19 Cells |
| title_full | MicroRNA Regulation for Inflammasomes in High Glucose-Treated ARPE-19 Cells |
| title_fullStr | MicroRNA Regulation for Inflammasomes in High Glucose-Treated ARPE-19 Cells |
| title_full_unstemmed | MicroRNA Regulation for Inflammasomes in High Glucose-Treated ARPE-19 Cells |
| title_short | MicroRNA Regulation for Inflammasomes in High Glucose-Treated ARPE-19 Cells |
| title_sort | microrna regulation for inflammasomes in high glucose treated arpe 19 cells |
| url | http://dx.doi.org/10.1155/2024/3654690 |
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