Laparoscopic Salpingectomy Regulates The Expression of Endometrial Homeobox Genes (HOXA9, HOXA10, HOXA11, HOXD10) and HOX Transcript Antisense Intergenic RNA in Women with Communicating Hydrosalpinx: A Prospective Study
Background: This study aimed to determine the alteration of endometrial expression levels of HOXA9/HOXA10/HOXA11/HOXD10 genes and HOX transcript antisense intergenic RNA (HOTAIR) in mid-luteal phase endometriumin patients with hydrosalpinx before and after salpingectomy.Materials and Methods: In thi...
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Royan Institute (ACECR), Tehran
2024-10-01
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| author | Fatemeh Zafarani Malek Soleimani Mehranjani Firouzeh Ghaffari Maryam Shahhoseini Seyed Mohammadi Ali Shariatzadeh |
| author_facet | Fatemeh Zafarani Malek Soleimani Mehranjani Firouzeh Ghaffari Maryam Shahhoseini Seyed Mohammadi Ali Shariatzadeh |
| author_sort | Fatemeh Zafarani |
| collection | DOAJ |
| description | Background: This study aimed to determine the alteration of endometrial expression levels of HOXA9/HOXA10/HOXA11/HOXD10 genes and HOX transcript antisense intergenic RNA (HOTAIR) in mid-luteal phase endometriumin patients with hydrosalpinx before and after salpingectomy.Materials and Methods: In this prospective study, 14 infertile women with unilateral hydrosalpinx who werescheduled for laparoscopic salpingectomy were evaluated. The presence of hydrosalpinx was confirmed by hysterosalpingographyor transvaginal 2D-ultrasonography. All patients had normal hormonal profiles, body mass index,and regular menstrual cycles identified by mid-luteal serum progesterone. Fourteen healthy fertile age-matchedwomen with a successful pregnancy history were considered the control group. Mid-luteal-phase endometrial biopsieswere performed at the time of surgery and during the mid-luteal phase of the fourth treatment cycle byPipelle. After tissue collection, RNA extraction and cDNA synthesis were performed. Real-time polymerase chainreaction (PCR) technique was used for quantitative gene expression of HOXA9/HOXA10/ HOXA11/ HOXD10 andlnc HOTAIR.Results: The endometrial expression of HOXA9 (P<0.001), HOXA10 (P=0.001), HOXA11 (P=0.003), and HOXD10(P=0.004) were significantly lower in the patients with hydrosalpinx compared to the controls. After salpingectomy,we observed a significant increase in the endometrial HOXA9 (P=0.006) and HOXA10 (P=0.023) mRNA expressionlevels compared to before salpingectomy samples. Similarly, a significant upregulation in endometrial HOXA11 (P=0.013) and HOXA10 mRNA expression levels (P=0.012) were detected in postoperative samples compared to preoperativetissue. Moreover, the lnc HOTAIR was significantly higher in the endometrium-induced hydrosalpinx fluidthan in controls (P=0.020), which had a 2.89-fold decrease following salpingectomy (P=0.010).Conclusion: Elevated endometrial lncRNA HOTAIR may disrupt the expression of endometrial receptivity HOXgenes in women with hydrosalpinx. However, our results failed to show a significant inverse correlation between HOTAIRand HOX genes due to limited sample size. Further studies with larger sample sizes are required to investigateHOTAIR inverse co-expression with HOX genes in these subjects. |
| format | Article |
| id | doaj-art-182cae895ff34d88bb8817fdfb7fc676 |
| institution | Kabale University |
| issn | 2008-076X 2008-0778 |
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| publishDate | 2024-10-01 |
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| spelling | doaj-art-182cae895ff34d88bb8817fdfb7fc6762024-11-20T11:28:46ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782024-10-0118434435110.22074/ijfs.2024.2026081.1655715033Laparoscopic Salpingectomy Regulates The Expression of Endometrial Homeobox Genes (HOXA9, HOXA10, HOXA11, HOXD10) and HOX Transcript Antisense Intergenic RNA in Women with Communicating Hydrosalpinx: A Prospective StudyFatemeh Zafarani0Malek Soleimani Mehranjani1Firouzeh Ghaffari2Maryam Shahhoseini3Seyed Mohammadi Ali Shariatzadeh4Department of Biology, Faculty of Science, Arak University, Arak, IranDepartment of Biology, Faculty of Science, Arak University, Arak, IranDepartment of Reproductive Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranDepartment of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranDepartment of Biology, Faculty of Science, Arak University, Arak, IranBackground: This study aimed to determine the alteration of endometrial expression levels of HOXA9/HOXA10/HOXA11/HOXD10 genes and HOX transcript antisense intergenic RNA (HOTAIR) in mid-luteal phase endometriumin patients with hydrosalpinx before and after salpingectomy.Materials and Methods: In this prospective study, 14 infertile women with unilateral hydrosalpinx who werescheduled for laparoscopic salpingectomy were evaluated. The presence of hydrosalpinx was confirmed by hysterosalpingographyor transvaginal 2D-ultrasonography. All patients had normal hormonal profiles, body mass index,and regular menstrual cycles identified by mid-luteal serum progesterone. Fourteen healthy fertile age-matchedwomen with a successful pregnancy history were considered the control group. Mid-luteal-phase endometrial biopsieswere performed at the time of surgery and during the mid-luteal phase of the fourth treatment cycle byPipelle. After tissue collection, RNA extraction and cDNA synthesis were performed. Real-time polymerase chainreaction (PCR) technique was used for quantitative gene expression of HOXA9/HOXA10/ HOXA11/ HOXD10 andlnc HOTAIR.Results: The endometrial expression of HOXA9 (P<0.001), HOXA10 (P=0.001), HOXA11 (P=0.003), and HOXD10(P=0.004) were significantly lower in the patients with hydrosalpinx compared to the controls. After salpingectomy,we observed a significant increase in the endometrial HOXA9 (P=0.006) and HOXA10 (P=0.023) mRNA expressionlevels compared to before salpingectomy samples. Similarly, a significant upregulation in endometrial HOXA11 (P=0.013) and HOXA10 mRNA expression levels (P=0.012) were detected in postoperative samples compared to preoperativetissue. Moreover, the lnc HOTAIR was significantly higher in the endometrium-induced hydrosalpinx fluidthan in controls (P=0.020), which had a 2.89-fold decrease following salpingectomy (P=0.010).Conclusion: Elevated endometrial lncRNA HOTAIR may disrupt the expression of endometrial receptivity HOXgenes in women with hydrosalpinx. However, our results failed to show a significant inverse correlation between HOTAIRand HOX genes due to limited sample size. Further studies with larger sample sizes are required to investigateHOTAIR inverse co-expression with HOX genes in these subjects.https://www.ijfs.ir/article_715033_201d9f0784d410499a4bfbf46b9d68a9.pdfendometrial receptivityhox antisense intergenic rna (hotair)hox geneshydrosalpinxsalpingectomy |
| spellingShingle | Fatemeh Zafarani Malek Soleimani Mehranjani Firouzeh Ghaffari Maryam Shahhoseini Seyed Mohammadi Ali Shariatzadeh Laparoscopic Salpingectomy Regulates The Expression of Endometrial Homeobox Genes (HOXA9, HOXA10, HOXA11, HOXD10) and HOX Transcript Antisense Intergenic RNA in Women with Communicating Hydrosalpinx: A Prospective Study International Journal of Fertility and Sterility endometrial receptivity hox antisense intergenic rna (hotair) hox genes hydrosalpinx salpingectomy |
| title | Laparoscopic Salpingectomy Regulates The Expression of Endometrial Homeobox Genes (HOXA9, HOXA10, HOXA11, HOXD10) and HOX Transcript Antisense Intergenic RNA in Women with Communicating Hydrosalpinx: A Prospective Study |
| title_full | Laparoscopic Salpingectomy Regulates The Expression of Endometrial Homeobox Genes (HOXA9, HOXA10, HOXA11, HOXD10) and HOX Transcript Antisense Intergenic RNA in Women with Communicating Hydrosalpinx: A Prospective Study |
| title_fullStr | Laparoscopic Salpingectomy Regulates The Expression of Endometrial Homeobox Genes (HOXA9, HOXA10, HOXA11, HOXD10) and HOX Transcript Antisense Intergenic RNA in Women with Communicating Hydrosalpinx: A Prospective Study |
| title_full_unstemmed | Laparoscopic Salpingectomy Regulates The Expression of Endometrial Homeobox Genes (HOXA9, HOXA10, HOXA11, HOXD10) and HOX Transcript Antisense Intergenic RNA in Women with Communicating Hydrosalpinx: A Prospective Study |
| title_short | Laparoscopic Salpingectomy Regulates The Expression of Endometrial Homeobox Genes (HOXA9, HOXA10, HOXA11, HOXD10) and HOX Transcript Antisense Intergenic RNA in Women with Communicating Hydrosalpinx: A Prospective Study |
| title_sort | laparoscopic salpingectomy regulates the expression of endometrial homeobox genes hoxa9 hoxa10 hoxa11 hoxd10 and hox transcript antisense intergenic rna in women with communicating hydrosalpinx a prospective study |
| topic | endometrial receptivity hox antisense intergenic rna (hotair) hox genes hydrosalpinx salpingectomy |
| url | https://www.ijfs.ir/article_715033_201d9f0784d410499a4bfbf46b9d68a9.pdf |
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