Preparation of Monodisperse Enrofloxacin Molecularly Imprinted Polymer Microspheres and Their Recognition Characteristics
This study presents a new strategy for the detection of enrofloxacin (ENR) in food samples by the use of monodisperse ENR molecularly imprinted polymers (MIPs). Using enrofloxacin as template molecule, methacrylic acid as functional monomer, and ethylene diglycidyl ether as cross-linker, surface mol...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2019/5970754 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849308817988255744 |
|---|---|
| author | Xiaoxiao Wang Yanqiang Zhou Yuling Niu Shanwen Zhao Bolin Gong |
| author_facet | Xiaoxiao Wang Yanqiang Zhou Yuling Niu Shanwen Zhao Bolin Gong |
| author_sort | Xiaoxiao Wang |
| collection | DOAJ |
| description | This study presents a new strategy for the detection of enrofloxacin (ENR) in food samples by the use of monodisperse ENR molecularly imprinted polymers (MIPs). Using enrofloxacin as template molecule, methacrylic acid as functional monomer, and ethylene diglycidyl ether as cross-linker, surface molecularly imprinted polymers (MIPs) were prepared on the surface of polymeric glycidyl methacrylate-ethylene glycol dimethacrylate (PGMA-EDMA) microspheres. The surface morphology and imprinting behavior of PGMA-EDMA@MIPs were investigated and optimized. Synthesized PGMA-EDMA@MIPs showed good physical and chemical stability and specific recognition toward fluoroquinolones. The introduction of PGMA-EDMA microspheres greatly increased the adsorption area of PGMA-EDMA@MIPs and increased the adsorption capacity of target molecules. The core shell structure increased the adsorption rate, and adsorption equilibrium was achieved within 6 min, much higher than that of MIPs synthesized by traditional methods. Enrofloxacin in milk samples was detected by molecular imprinting solid phase extraction (MISPE) combined with high performance liquid chromatography (HPLC). Implementing this method resulted in a recovery rate of 94.6~109.6% with a relative standard deviation (RSD) of less than 3.2%. The limit of detection (LOD) of this method was identified as three times the signal-to-noise ratio (10 μg/L). In summary, this work proposed a sensitive, rapid, and convenient method for the determination of trace ENR in food samples. |
| format | Article |
| id | doaj-art-17f6f837bd7b408eab3d3f29d2e4c861 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-17f6f837bd7b408eab3d3f29d2e4c8612025-08-20T03:54:21ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792019-01-01201910.1155/2019/59707545970754Preparation of Monodisperse Enrofloxacin Molecularly Imprinted Polymer Microspheres and Their Recognition CharacteristicsXiaoxiao Wang0Yanqiang Zhou1Yuling Niu2Shanwen Zhao3Bolin Gong4College of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, ChinaCollege of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, ChinaNingxia Entry-Exit Inspection and Quarantine Bureau Comprehensive Technology Center, Yinchuan, Ningxia, ChinaCollege of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, ChinaCollege of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, ChinaThis study presents a new strategy for the detection of enrofloxacin (ENR) in food samples by the use of monodisperse ENR molecularly imprinted polymers (MIPs). Using enrofloxacin as template molecule, methacrylic acid as functional monomer, and ethylene diglycidyl ether as cross-linker, surface molecularly imprinted polymers (MIPs) were prepared on the surface of polymeric glycidyl methacrylate-ethylene glycol dimethacrylate (PGMA-EDMA) microspheres. The surface morphology and imprinting behavior of PGMA-EDMA@MIPs were investigated and optimized. Synthesized PGMA-EDMA@MIPs showed good physical and chemical stability and specific recognition toward fluoroquinolones. The introduction of PGMA-EDMA microspheres greatly increased the adsorption area of PGMA-EDMA@MIPs and increased the adsorption capacity of target molecules. The core shell structure increased the adsorption rate, and adsorption equilibrium was achieved within 6 min, much higher than that of MIPs synthesized by traditional methods. Enrofloxacin in milk samples was detected by molecular imprinting solid phase extraction (MISPE) combined with high performance liquid chromatography (HPLC). Implementing this method resulted in a recovery rate of 94.6~109.6% with a relative standard deviation (RSD) of less than 3.2%. The limit of detection (LOD) of this method was identified as three times the signal-to-noise ratio (10 μg/L). In summary, this work proposed a sensitive, rapid, and convenient method for the determination of trace ENR in food samples.http://dx.doi.org/10.1155/2019/5970754 |
| spellingShingle | Xiaoxiao Wang Yanqiang Zhou Yuling Niu Shanwen Zhao Bolin Gong Preparation of Monodisperse Enrofloxacin Molecularly Imprinted Polymer Microspheres and Their Recognition Characteristics International Journal of Analytical Chemistry |
| title | Preparation of Monodisperse Enrofloxacin Molecularly Imprinted Polymer Microspheres and Their Recognition Characteristics |
| title_full | Preparation of Monodisperse Enrofloxacin Molecularly Imprinted Polymer Microspheres and Their Recognition Characteristics |
| title_fullStr | Preparation of Monodisperse Enrofloxacin Molecularly Imprinted Polymer Microspheres and Their Recognition Characteristics |
| title_full_unstemmed | Preparation of Monodisperse Enrofloxacin Molecularly Imprinted Polymer Microspheres and Their Recognition Characteristics |
| title_short | Preparation of Monodisperse Enrofloxacin Molecularly Imprinted Polymer Microspheres and Their Recognition Characteristics |
| title_sort | preparation of monodisperse enrofloxacin molecularly imprinted polymer microspheres and their recognition characteristics |
| url | http://dx.doi.org/10.1155/2019/5970754 |
| work_keys_str_mv | AT xiaoxiaowang preparationofmonodisperseenrofloxacinmolecularlyimprintedpolymermicrospheresandtheirrecognitioncharacteristics AT yanqiangzhou preparationofmonodisperseenrofloxacinmolecularlyimprintedpolymermicrospheresandtheirrecognitioncharacteristics AT yulingniu preparationofmonodisperseenrofloxacinmolecularlyimprintedpolymermicrospheresandtheirrecognitioncharacteristics AT shanwenzhao preparationofmonodisperseenrofloxacinmolecularlyimprintedpolymermicrospheresandtheirrecognitioncharacteristics AT bolingong preparationofmonodisperseenrofloxacinmolecularlyimprintedpolymermicrospheresandtheirrecognitioncharacteristics |