Optimization of Intracellular Polysaccharide Extraction Process and Analysis of Antioxidant Activity in Co-cultures of Sanghuangporus vaninii and Pleurotus sapidus
In this study, an ultrasonic synergistic semi-bionic method was explored to extract intracellular polysaccharides from Sanghuangporus vaninii and Pleurotus sapidus liquid co-cultures. By conducting single-factor experiments, Plackett-Burman design, and response surface design, the impact of 12 facto...
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The editorial department of Science and Technology of Food Industry
2025-01-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024020046 |
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| author | Yuantian LU Di LIU |
| author_facet | Yuantian LU Di LIU |
| author_sort | Yuantian LU |
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| description | In this study, an ultrasonic synergistic semi-bionic method was explored to extract intracellular polysaccharides from Sanghuangporus vaninii and Pleurotus sapidus liquid co-cultures. By conducting single-factor experiments, Plackett-Burman design, and response surface design, the impact of 12 factors on co-culture intracellular polysaccharide (CC-IPS) extraction content, including extraction material-liquid ratios, extraction time, extraction temperature, and extraction solution pH were investigated. The antioxidant capacity of CC-IPS in vitro was evaluated by the DPPH and ABTS+ radical scavenging rates. Results showed that, the optimal extraction process parameters for the first, second, and third extractions, respectively, included material-to-liquid ratios of 1:17, 1:40, and 1:30 (g/mL), extraction times of 31, 30, and 30 min, extraction temperatures of 44, 40, and 50 ℃, and extraction solution pH levels of 3, 8, and 10. Under these optimal conditions, the CC-IPS extraction content was 86.4921±1.1924 mg/g, closed to the predicted value of 85.3981 mg/g. Additionally, the IC50 values of CC-IPS in the DPPH and ABTS+ free radical scavenging assays were determined to be 0.2425 and 0.1376 mg/mL, respectively. This study offers a theoretical foundation for the efficient utilization of intracellular polysaccharides in liquid fungal co-cultures. |
| format | Article |
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| institution | Kabale University |
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| language | zho |
| publishDate | 2025-01-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| spelling | doaj-art-17b71ff1af3a4dac9add6125ed16d2332025-01-10T06:49:30ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-01-0146220821710.13386/j.issn1002-0306.20240200462024020046-2Optimization of Intracellular Polysaccharide Extraction Process and Analysis of Antioxidant Activity in Co-cultures of Sanghuangporus vaninii and Pleurotus sapidusYuantian LU0Di LIU1Agricultural College, Yanbian University, Yanji 133002, ChinaAgricultural College, Yanbian University, Yanji 133002, ChinaIn this study, an ultrasonic synergistic semi-bionic method was explored to extract intracellular polysaccharides from Sanghuangporus vaninii and Pleurotus sapidus liquid co-cultures. By conducting single-factor experiments, Plackett-Burman design, and response surface design, the impact of 12 factors on co-culture intracellular polysaccharide (CC-IPS) extraction content, including extraction material-liquid ratios, extraction time, extraction temperature, and extraction solution pH were investigated. The antioxidant capacity of CC-IPS in vitro was evaluated by the DPPH and ABTS+ radical scavenging rates. Results showed that, the optimal extraction process parameters for the first, second, and third extractions, respectively, included material-to-liquid ratios of 1:17, 1:40, and 1:30 (g/mL), extraction times of 31, 30, and 30 min, extraction temperatures of 44, 40, and 50 ℃, and extraction solution pH levels of 3, 8, and 10. Under these optimal conditions, the CC-IPS extraction content was 86.4921±1.1924 mg/g, closed to the predicted value of 85.3981 mg/g. Additionally, the IC50 values of CC-IPS in the DPPH and ABTS+ free radical scavenging assays were determined to be 0.2425 and 0.1376 mg/mL, respectively. This study offers a theoretical foundation for the efficient utilization of intracellular polysaccharides in liquid fungal co-cultures.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024020046sanghuangporus vaniniipleurotus sapidussemi-bionic extractionco-culturepolysaccharideantioxidant activity |
| spellingShingle | Yuantian LU Di LIU Optimization of Intracellular Polysaccharide Extraction Process and Analysis of Antioxidant Activity in Co-cultures of Sanghuangporus vaninii and Pleurotus sapidus Shipin gongye ke-ji sanghuangporus vaninii pleurotus sapidus semi-bionic extraction co-culture polysaccharide antioxidant activity |
| title | Optimization of Intracellular Polysaccharide Extraction Process and Analysis of Antioxidant Activity in Co-cultures of Sanghuangporus vaninii and Pleurotus sapidus |
| title_full | Optimization of Intracellular Polysaccharide Extraction Process and Analysis of Antioxidant Activity in Co-cultures of Sanghuangporus vaninii and Pleurotus sapidus |
| title_fullStr | Optimization of Intracellular Polysaccharide Extraction Process and Analysis of Antioxidant Activity in Co-cultures of Sanghuangporus vaninii and Pleurotus sapidus |
| title_full_unstemmed | Optimization of Intracellular Polysaccharide Extraction Process and Analysis of Antioxidant Activity in Co-cultures of Sanghuangporus vaninii and Pleurotus sapidus |
| title_short | Optimization of Intracellular Polysaccharide Extraction Process and Analysis of Antioxidant Activity in Co-cultures of Sanghuangporus vaninii and Pleurotus sapidus |
| title_sort | optimization of intracellular polysaccharide extraction process and analysis of antioxidant activity in co cultures of sanghuangporus vaninii and pleurotus sapidus |
| topic | sanghuangporus vaninii pleurotus sapidus semi-bionic extraction co-culture polysaccharide antioxidant activity |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024020046 |
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