Pt@ZnCo<sub>2</sub>O<sub>4</sub> Microspheres as Peroxidase Mimics: Enhanced Catalytic Activity and Application for L-Cysteine Detection
Compared to natural enzymes, the development of efficient artificial simulated enzymes, such as those based on bimetallic materials with high catalytic activity and good stability, is an important way until now. Herein, we employed ZnCo<sub>2</sub>O<sub>4</sub> microspheres a...
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2025-01-01
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| author | Shuqi Liao Weisen Deng Feng Yang Jutao Zhou Ling Wu Donghong Yu Zhong Cao |
| author_facet | Shuqi Liao Weisen Deng Feng Yang Jutao Zhou Ling Wu Donghong Yu Zhong Cao |
| author_sort | Shuqi Liao |
| collection | DOAJ |
| description | Compared to natural enzymes, the development of efficient artificial simulated enzymes, such as those based on bimetallic materials with high catalytic activity and good stability, is an important way until now. Herein, we employed ZnCo<sub>2</sub>O<sub>4</sub> microspheres as carriers to synthesize Pt-doped composites with different amounts using a one-pot method. The morphology and structure of the synthesized materials were characterized using XRD, SEM, BET, FT-IR, XPS, and Zeta potential techniques. It was found that Pt<sup>0</sup> adhered well to the surface of ZnCo<sub>2</sub>O<sub>4</sub> microspheres, with a 12.5% Pt doped ratio exhibiting abundant oxygen vacancies, excellent substrate affinity, and high peroxidase-like activity. Using fluorescent probes and electrochemical methods, the peroxidase-like catalytic mechanism has been explored that Pt@ZnCo<sub>2</sub>O<sub>4</sub> microspheres can accelerate the electron transfer between H<sub>2</sub>O<sub>2</sub> and 3,3′,5,5′-tetramethylbenzidine (TMB). Based on the optimal loading ratio of 12.5% of Pt@ZnCo<sub>2</sub>O<sub>4</sub>, a colorimetric sensor for visual detection of L-cysteine (L-Cys) was constructed, exhibiting a wide linear range of 0.1~50 µM and a low detection limit of 0.0163 µM. The sensor possesses good selectivity, reusability, and usage stability, which can be well applied to the determination of L-Cys in health product capsules with recovery rates of 96.9%~103.7% and RSD of 1.07%~6.50%. This work broadens the application prospects of spinel materials such as ZnCo<sub>2</sub>O<sub>4</sub> in the field of biological analysis and also provides inspiration for the development of new artificial simulated enzymes. |
| format | Article |
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| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj-art-1728241ce1ee4b60a53e848dcf5e1ff72025-01-10T13:19:09ZengMDPI AGMolecules1420-30492025-01-0130118710.3390/molecules30010187Pt@ZnCo<sub>2</sub>O<sub>4</sub> Microspheres as Peroxidase Mimics: Enhanced Catalytic Activity and Application for L-Cysteine DetectionShuqi Liao0Weisen Deng1Feng Yang2Jutao Zhou3Ling Wu4Donghong Yu5Zhong Cao6Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation & Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha 410114, ChinaHunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation & Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha 410114, ChinaHunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation & Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha 410114, ChinaHunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation & Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha 410114, ChinaHunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation & Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha 410114, ChinaDepartment of Chemistry and Bioscience, Aalborg University, DK-9220 Aalborg, DenmarkHunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation & Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha 410114, ChinaCompared to natural enzymes, the development of efficient artificial simulated enzymes, such as those based on bimetallic materials with high catalytic activity and good stability, is an important way until now. Herein, we employed ZnCo<sub>2</sub>O<sub>4</sub> microspheres as carriers to synthesize Pt-doped composites with different amounts using a one-pot method. The morphology and structure of the synthesized materials were characterized using XRD, SEM, BET, FT-IR, XPS, and Zeta potential techniques. It was found that Pt<sup>0</sup> adhered well to the surface of ZnCo<sub>2</sub>O<sub>4</sub> microspheres, with a 12.5% Pt doped ratio exhibiting abundant oxygen vacancies, excellent substrate affinity, and high peroxidase-like activity. Using fluorescent probes and electrochemical methods, the peroxidase-like catalytic mechanism has been explored that Pt@ZnCo<sub>2</sub>O<sub>4</sub> microspheres can accelerate the electron transfer between H<sub>2</sub>O<sub>2</sub> and 3,3′,5,5′-tetramethylbenzidine (TMB). Based on the optimal loading ratio of 12.5% of Pt@ZnCo<sub>2</sub>O<sub>4</sub>, a colorimetric sensor for visual detection of L-cysteine (L-Cys) was constructed, exhibiting a wide linear range of 0.1~50 µM and a low detection limit of 0.0163 µM. The sensor possesses good selectivity, reusability, and usage stability, which can be well applied to the determination of L-Cys in health product capsules with recovery rates of 96.9%~103.7% and RSD of 1.07%~6.50%. This work broadens the application prospects of spinel materials such as ZnCo<sub>2</sub>O<sub>4</sub> in the field of biological analysis and also provides inspiration for the development of new artificial simulated enzymes.https://www.mdpi.com/1420-3049/30/1/187artificial mimetic enzymePt@ZnCo<sub>2</sub>O<sub>4</sub> microspheresperoxidase-like enzymesL-cysteinecolorimetric detection |
| spellingShingle | Shuqi Liao Weisen Deng Feng Yang Jutao Zhou Ling Wu Donghong Yu Zhong Cao Pt@ZnCo<sub>2</sub>O<sub>4</sub> Microspheres as Peroxidase Mimics: Enhanced Catalytic Activity and Application for L-Cysteine Detection Molecules artificial mimetic enzyme Pt@ZnCo<sub>2</sub>O<sub>4</sub> microspheres peroxidase-like enzymes L-cysteine colorimetric detection |
| title | Pt@ZnCo<sub>2</sub>O<sub>4</sub> Microspheres as Peroxidase Mimics: Enhanced Catalytic Activity and Application for L-Cysteine Detection |
| title_full | Pt@ZnCo<sub>2</sub>O<sub>4</sub> Microspheres as Peroxidase Mimics: Enhanced Catalytic Activity and Application for L-Cysteine Detection |
| title_fullStr | Pt@ZnCo<sub>2</sub>O<sub>4</sub> Microspheres as Peroxidase Mimics: Enhanced Catalytic Activity and Application for L-Cysteine Detection |
| title_full_unstemmed | Pt@ZnCo<sub>2</sub>O<sub>4</sub> Microspheres as Peroxidase Mimics: Enhanced Catalytic Activity and Application for L-Cysteine Detection |
| title_short | Pt@ZnCo<sub>2</sub>O<sub>4</sub> Microspheres as Peroxidase Mimics: Enhanced Catalytic Activity and Application for L-Cysteine Detection |
| title_sort | pt znco sub 2 sub o sub 4 sub microspheres as peroxidase mimics enhanced catalytic activity and application for l cysteine detection |
| topic | artificial mimetic enzyme Pt@ZnCo<sub>2</sub>O<sub>4</sub> microspheres peroxidase-like enzymes L-cysteine colorimetric detection |
| url | https://www.mdpi.com/1420-3049/30/1/187 |
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