Antimicrobial Profiling of <i>Piper betle</i> L. and <i>Piper nigrum</i> L. Against Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA): Integrative Analysis of Bioactive Compounds Based on FT-IR, GC-MS, and Molecular Docking Studies
This study explored the antimicrobial potential of <i>Piper betle</i> L. (PBL) and <i>Piper nigrum</i> L. (PNL) extracts against MRSA. Plant parts including stem, leaf, and fruit were extracted using aquadest, methanol, and hexane, resulting in 18 distinct extracts. FT-IR com...
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2024-11-01
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| author | Budiman Yasir Suwahyuni Mus Sitti Rahimah Rein Mostatian Tandiongan Kasandra Putri Klara Nurul Afrida Nur Rezky Khairun Nisaa Risna Risna Agum Wahyudha Jur Gemini Alam Abdul Rohman |
| author_facet | Budiman Yasir Suwahyuni Mus Sitti Rahimah Rein Mostatian Tandiongan Kasandra Putri Klara Nurul Afrida Nur Rezky Khairun Nisaa Risna Risna Agum Wahyudha Jur Gemini Alam Abdul Rohman |
| author_sort | Budiman Yasir |
| collection | DOAJ |
| description | This study explored the antimicrobial potential of <i>Piper betle</i> L. (PBL) and <i>Piper nigrum</i> L. (PNL) extracts against MRSA. Plant parts including stem, leaf, and fruit were extracted using aquadest, methanol, and hexane, resulting in 18 distinct extracts. FT-IR combined with cluster analysis (CA) categorized the extracts, and anti-MRSA activity was assessed through the paper disk diffusion method. The most potent extracts were further analyzed using GC-MS to identify bioactive compounds. Additionally, molecular docking studies were conducted for MRSA protein targets (4DKI, 6H5O, and 4CJN). The hexane extract of PNL and the aqueous extract of PBL fruit showed the strongest inhibitory effects. GC-MS identified piperine (14.22%) and diisooctyl phthalate (14.67%) as major compounds, with piperolein B, piperanine, β-caryophyllene oxide, and α-caryophylladienol as minor compounds in the hexane extract of PNL, while hydroxychavicol (81.89%) and chavibetol (12.01%) were predominant in the aquadest extract of PBL. Molecular docking revealed that piperolein B and piperine had strong binding affinities to MRSA proteins 4DKI, 6H5O, and 4CJN, comparable to ciprofloxacin. In conclusion, this study confirms the potential of PBL and PNL as sources of novel anti-MRSA agents, supporting further research to develop new therapies. |
| format | Article |
| id | doaj-art-16f43fecf94b4c6787247bc28bf83f86 |
| institution | Kabale University |
| issn | 2297-8739 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Separations |
| spelling | doaj-art-16f43fecf94b4c6787247bc28bf83f862024-11-26T18:21:59ZengMDPI AGSeparations2297-87392024-11-01111132210.3390/separations11110322Antimicrobial Profiling of <i>Piper betle</i> L. and <i>Piper nigrum</i> L. Against Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA): Integrative Analysis of Bioactive Compounds Based on FT-IR, GC-MS, and Molecular Docking StudiesBudiman Yasir0Suwahyuni Mus1Sitti Rahimah2Rein Mostatian Tandiongan3Kasandra Putri Klara4Nurul Afrida5Nur Rezky Khairun Nisaa6Risna Risna7Agum Wahyudha Jur8Gemini Alam9Abdul Rohman10Faculty of Health Sciences, Almarisah Madani University, Makassar 90245, IndonesiaFaculty of Health Sciences, Almarisah Madani University, Makassar 90245, IndonesiaFaculty of Health Sciences, Almarisah Madani University, Makassar 90245, IndonesiaFaculty of Health Sciences, Almarisah Madani University, Makassar 90245, IndonesiaFaculty of Health Sciences, Almarisah Madani University, Makassar 90245, IndonesiaFaculty of Health Sciences, Almarisah Madani University, Makassar 90245, IndonesiaFaculty of Pharmacy, Mulawarman University, Samarinda 75242, IndonesiaSekolah Tinggi Ilmu Kesehatan Jayapura, Papua 99352, IndonesiaFaculty of Pharmacy, Hasanuddin University, Makassar 90245, IndonesiaFaculty of Pharmacy, Hasanuddin University, Makassar 90245, IndonesiaFaculty of Pharmacy, Gadjah Mada University, Yogyakarta 55281, IndonesiaThis study explored the antimicrobial potential of <i>Piper betle</i> L. (PBL) and <i>Piper nigrum</i> L. (PNL) extracts against MRSA. Plant parts including stem, leaf, and fruit were extracted using aquadest, methanol, and hexane, resulting in 18 distinct extracts. FT-IR combined with cluster analysis (CA) categorized the extracts, and anti-MRSA activity was assessed through the paper disk diffusion method. The most potent extracts were further analyzed using GC-MS to identify bioactive compounds. Additionally, molecular docking studies were conducted for MRSA protein targets (4DKI, 6H5O, and 4CJN). The hexane extract of PNL and the aqueous extract of PBL fruit showed the strongest inhibitory effects. GC-MS identified piperine (14.22%) and diisooctyl phthalate (14.67%) as major compounds, with piperolein B, piperanine, β-caryophyllene oxide, and α-caryophylladienol as minor compounds in the hexane extract of PNL, while hydroxychavicol (81.89%) and chavibetol (12.01%) were predominant in the aquadest extract of PBL. Molecular docking revealed that piperolein B and piperine had strong binding affinities to MRSA proteins 4DKI, 6H5O, and 4CJN, comparable to ciprofloxacin. In conclusion, this study confirms the potential of PBL and PNL as sources of novel anti-MRSA agents, supporting further research to develop new therapies.https://www.mdpi.com/2297-8739/11/11/322<i>Piper</i> extractscluster analysisMRSAFourier transform infraredgas chromatography-mass spectrometryin silico studies |
| spellingShingle | Budiman Yasir Suwahyuni Mus Sitti Rahimah Rein Mostatian Tandiongan Kasandra Putri Klara Nurul Afrida Nur Rezky Khairun Nisaa Risna Risna Agum Wahyudha Jur Gemini Alam Abdul Rohman Antimicrobial Profiling of <i>Piper betle</i> L. and <i>Piper nigrum</i> L. Against Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA): Integrative Analysis of Bioactive Compounds Based on FT-IR, GC-MS, and Molecular Docking Studies Separations <i>Piper</i> extracts cluster analysis MRSA Fourier transform infrared gas chromatography-mass spectrometry in silico studies |
| title | Antimicrobial Profiling of <i>Piper betle</i> L. and <i>Piper nigrum</i> L. Against Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA): Integrative Analysis of Bioactive Compounds Based on FT-IR, GC-MS, and Molecular Docking Studies |
| title_full | Antimicrobial Profiling of <i>Piper betle</i> L. and <i>Piper nigrum</i> L. Against Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA): Integrative Analysis of Bioactive Compounds Based on FT-IR, GC-MS, and Molecular Docking Studies |
| title_fullStr | Antimicrobial Profiling of <i>Piper betle</i> L. and <i>Piper nigrum</i> L. Against Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA): Integrative Analysis of Bioactive Compounds Based on FT-IR, GC-MS, and Molecular Docking Studies |
| title_full_unstemmed | Antimicrobial Profiling of <i>Piper betle</i> L. and <i>Piper nigrum</i> L. Against Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA): Integrative Analysis of Bioactive Compounds Based on FT-IR, GC-MS, and Molecular Docking Studies |
| title_short | Antimicrobial Profiling of <i>Piper betle</i> L. and <i>Piper nigrum</i> L. Against Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA): Integrative Analysis of Bioactive Compounds Based on FT-IR, GC-MS, and Molecular Docking Studies |
| title_sort | antimicrobial profiling of i piper betle i l and i piper nigrum i l against methicillin resistant i staphylococcus aureus i mrsa integrative analysis of bioactive compounds based on ft ir gc ms and molecular docking studies |
| topic | <i>Piper</i> extracts cluster analysis MRSA Fourier transform infrared gas chromatography-mass spectrometry in silico studies |
| url | https://www.mdpi.com/2297-8739/11/11/322 |
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