DNA-based detection of Rat in the meatballs product using a real-time polymerase chain reaction method
The meat-based products are highly susceptible to counterfeiting, primarily due to high consumer demand of meat derivative products, such as meatballs. This demand creates opportunities for food fraud by specific industries, including adulterating meat with non-halal species, such as rats. This res...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universitas Ahmad Dahlan
2024-11-01
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| Series: | Pharmaciana |
| Online Access: | https://journal.uad.ac.id/index.php/PHARMACIANA/article/view/27850 |
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| author | Etin Diah Permanasari Hadi Sunaryo Adia Putra Wirman Nuriza Rahmadini Savira Yustinah Aggasy Nurul Azmah Nikmatullah |
| author_facet | Etin Diah Permanasari Hadi Sunaryo Adia Putra Wirman Nuriza Rahmadini Savira Yustinah Aggasy Nurul Azmah Nikmatullah |
| author_sort | Etin Diah Permanasari |
| collection | DOAJ |
| description |
The meat-based products are highly susceptible to counterfeiting, primarily due to high consumer demand of meat derivative products, such as meatballs. This demand creates opportunities for food fraud by specific industries, including adulterating meat with non-halal species, such as rats. This research aimed to detect rat meat contamination in meatball samples from the Indonesian local market using Real-time Polymerase Chain Reaction (RT-PCR). The RT-PCR amplification involved an initial denaturation step at 95°C for 3 minutes, followed by denaturation at 95°C for 15 seconds, and annealing/extension at 60°C for 1 minute. The rat-specific probe primer included in the kit produced an increasing curve in the External Positive Control (EPC) with a Ct value of 27.22, and no amplification occurred in the Negative Control (NTC). The analysis of 30 samples from meatball vendors yielded negative results, as there was no increase in the FAM (rat) curve, indicating that none of the meatballs were contaminated with rat DNA.
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| format | Article |
| id | doaj-art-16c79b5ba8a045aabf1e697510163196 |
| institution | Kabale University |
| issn | 2088-4559 2477-0256 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Universitas Ahmad Dahlan |
| record_format | Article |
| series | Pharmaciana |
| spelling | doaj-art-16c79b5ba8a045aabf1e6975101631962025-01-12T00:37:12ZengUniversitas Ahmad DahlanPharmaciana2088-45592477-02562024-11-0114310.12928/pharmaciana.v14i3.2785020123DNA-based detection of Rat in the meatballs product using a real-time polymerase chain reaction methodEtin Diah Permanasari0Hadi Sunaryo1Adia Putra Wirman2Nuriza Rahmadini3Savira Yustinah Aggasy4Nurul Azmah Nikmatullah5Universitas Muhammadiyah Prof. DR. HAMKAUniversitas Muhammadiyah Prof. DR. HAMKAUniversitas Muhammadiyah Prof. DR. HAMKAUniversitas Muhammadiyah Prof. DR. HAMKAUniversitas Muhammadiyah Prof. DR. HAMKAUniversitas Muhammadiyah Prof DR HAMKA The meat-based products are highly susceptible to counterfeiting, primarily due to high consumer demand of meat derivative products, such as meatballs. This demand creates opportunities for food fraud by specific industries, including adulterating meat with non-halal species, such as rats. This research aimed to detect rat meat contamination in meatball samples from the Indonesian local market using Real-time Polymerase Chain Reaction (RT-PCR). The RT-PCR amplification involved an initial denaturation step at 95°C for 3 minutes, followed by denaturation at 95°C for 15 seconds, and annealing/extension at 60°C for 1 minute. The rat-specific probe primer included in the kit produced an increasing curve in the External Positive Control (EPC) with a Ct value of 27.22, and no amplification occurred in the Negative Control (NTC). The analysis of 30 samples from meatball vendors yielded negative results, as there was no increase in the FAM (rat) curve, indicating that none of the meatballs were contaminated with rat DNA. https://journal.uad.ac.id/index.php/PHARMACIANA/article/view/27850 |
| spellingShingle | Etin Diah Permanasari Hadi Sunaryo Adia Putra Wirman Nuriza Rahmadini Savira Yustinah Aggasy Nurul Azmah Nikmatullah DNA-based detection of Rat in the meatballs product using a real-time polymerase chain reaction method Pharmaciana |
| title | DNA-based detection of Rat in the meatballs product using a real-time polymerase chain reaction method |
| title_full | DNA-based detection of Rat in the meatballs product using a real-time polymerase chain reaction method |
| title_fullStr | DNA-based detection of Rat in the meatballs product using a real-time polymerase chain reaction method |
| title_full_unstemmed | DNA-based detection of Rat in the meatballs product using a real-time polymerase chain reaction method |
| title_short | DNA-based detection of Rat in the meatballs product using a real-time polymerase chain reaction method |
| title_sort | dna based detection of rat in the meatballs product using a real time polymerase chain reaction method |
| url | https://journal.uad.ac.id/index.php/PHARMACIANA/article/view/27850 |
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