A colorimetric detection of creatine kinase for monitoring of physiological conditions in sports injury rehabilitation based on metal nanoparticle structures
This study focused on detection of creatine kinase (CK) as a vital biomarker for diagnosing and monitoring various medical conditions, including myocardial infarction and muscle disorders. This study presents the development of a PAH-aptamer/AgNP-based colorimetric biosensor for the sensitive and sp...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-05-01
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| Series: | Alexandria Engineering Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110016825002753 |
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| author | Ruqiong Wei Shenyi Lu Tao Wang Zhanghui Lin Jiaying Hu Jinyuan Guo Xinze Jiang Raquel Alarcón Rodríguez Yunshan Zhang |
| author_facet | Ruqiong Wei Shenyi Lu Tao Wang Zhanghui Lin Jiaying Hu Jinyuan Guo Xinze Jiang Raquel Alarcón Rodríguez Yunshan Zhang |
| author_sort | Ruqiong Wei |
| collection | DOAJ |
| description | This study focused on detection of creatine kinase (CK) as a vital biomarker for diagnosing and monitoring various medical conditions, including myocardial infarction and muscle disorders. This study presents the development of a PAH-aptamer/AgNP-based colorimetric biosensor for the sensitive and specific detection of CK-MM. Silver nanoparticles (AgNPs) were synthesized via a bottom-up technique and functionalized with poly(allylamine hydrochloride) (PAH) and a thiolated CK-MM aptamer. The FTIR analysis indicated successful conjugation of PAH and aptamer to the AgNPs. The PAH-Aptamer/AgNP-based colorimetry assay showed a high sensitivity with a detection limit of 9 pg/mL and a wide linear detection range from 0.1 ng/mL to 104 ng/mL. Comparative studies with an ELISA kit demonstrated good agreement, with recovery rates ranging from 96.00 % to 99.10 % and relative standard deviations between 3.72 % and 4.78 %. The PAH-aptamer/AgNP-based biosensor offers a rapid, cost-effective, and practical alternative to traditional detection methods, making it a valuable tool for clinical diagnostics and monitoring physiological conditions in sports injury rehabilitation. This study underscores the potential of using PAH-aptamer/AgNPs in colorimetric sensing systems, providing a robust platform for early diagnosis and monitoring of CK-related conditions with high sensitivity, specificity, and practicality. |
| format | Article |
| id | doaj-art-16bacbb0902f4bc6961e52d69d0f14d5 |
| institution | Kabale University |
| issn | 1110-0168 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Alexandria Engineering Journal |
| spelling | doaj-art-16bacbb0902f4bc6961e52d69d0f14d52025-08-20T03:49:32ZengElsevierAlexandria Engineering Journal1110-01682025-05-0112132733610.1016/j.aej.2025.02.102A colorimetric detection of creatine kinase for monitoring of physiological conditions in sports injury rehabilitation based on metal nanoparticle structuresRuqiong Wei0Shenyi Lu1Tao Wang2Zhanghui Lin3Jiaying Hu4Jinyuan Guo5Xinze Jiang6Raquel Alarcón Rodríguez7Yunshan Zhang8Department of Rehabilitation Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, ChinaDepartment of Rehabilitation, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, China; Key Laboratory of Research and Development on Clinical Molecular Diagnosis for High-Incidence Diseases of Baise, Baise, ChinaDepartment of Bone and Joint Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, ChinaGuangxi Medical University, Nanning 530021, ChinaGuangxi Medical University, Nanning 530021, ChinaGuangxi Medical University, Nanning 530021, ChinaGuangxi Medical University, Nanning 530021, ChinaFaculty of Health Sciences, University of Almeria, Carretera Sacramento s/n, La Canada, Almería 04120, Spain; Corresponding authors.Department of Rehabilitation Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China; Corresponding authors.This study focused on detection of creatine kinase (CK) as a vital biomarker for diagnosing and monitoring various medical conditions, including myocardial infarction and muscle disorders. This study presents the development of a PAH-aptamer/AgNP-based colorimetric biosensor for the sensitive and specific detection of CK-MM. Silver nanoparticles (AgNPs) were synthesized via a bottom-up technique and functionalized with poly(allylamine hydrochloride) (PAH) and a thiolated CK-MM aptamer. The FTIR analysis indicated successful conjugation of PAH and aptamer to the AgNPs. The PAH-Aptamer/AgNP-based colorimetry assay showed a high sensitivity with a detection limit of 9 pg/mL and a wide linear detection range from 0.1 ng/mL to 104 ng/mL. Comparative studies with an ELISA kit demonstrated good agreement, with recovery rates ranging from 96.00 % to 99.10 % and relative standard deviations between 3.72 % and 4.78 %. The PAH-aptamer/AgNP-based biosensor offers a rapid, cost-effective, and practical alternative to traditional detection methods, making it a valuable tool for clinical diagnostics and monitoring physiological conditions in sports injury rehabilitation. This study underscores the potential of using PAH-aptamer/AgNPs in colorimetric sensing systems, providing a robust platform for early diagnosis and monitoring of CK-related conditions with high sensitivity, specificity, and practicality.http://www.sciencedirect.com/science/article/pii/S1110016825002753Creatine kinaseSilver nanoparticlesPoly(allylamine hydrochloride)Colorimetric biosensorClinical diagnostics |
| spellingShingle | Ruqiong Wei Shenyi Lu Tao Wang Zhanghui Lin Jiaying Hu Jinyuan Guo Xinze Jiang Raquel Alarcón Rodríguez Yunshan Zhang A colorimetric detection of creatine kinase for monitoring of physiological conditions in sports injury rehabilitation based on metal nanoparticle structures Alexandria Engineering Journal Creatine kinase Silver nanoparticles Poly(allylamine hydrochloride) Colorimetric biosensor Clinical diagnostics |
| title | A colorimetric detection of creatine kinase for monitoring of physiological conditions in sports injury rehabilitation based on metal nanoparticle structures |
| title_full | A colorimetric detection of creatine kinase for monitoring of physiological conditions in sports injury rehabilitation based on metal nanoparticle structures |
| title_fullStr | A colorimetric detection of creatine kinase for monitoring of physiological conditions in sports injury rehabilitation based on metal nanoparticle structures |
| title_full_unstemmed | A colorimetric detection of creatine kinase for monitoring of physiological conditions in sports injury rehabilitation based on metal nanoparticle structures |
| title_short | A colorimetric detection of creatine kinase for monitoring of physiological conditions in sports injury rehabilitation based on metal nanoparticle structures |
| title_sort | colorimetric detection of creatine kinase for monitoring of physiological conditions in sports injury rehabilitation based on metal nanoparticle structures |
| topic | Creatine kinase Silver nanoparticles Poly(allylamine hydrochloride) Colorimetric biosensor Clinical diagnostics |
| url | http://www.sciencedirect.com/science/article/pii/S1110016825002753 |
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