Characterizing Opine Physiological and Genetic Diversity in Moroccan strains of Allorhizobium vitis, the Causal Agent of Grapevine Crown Gall
This study investigates the physiological and genetic diversity of Allorhizobium vitis strains in Morocco, focusing on their ability to produce and utilize opines as sources of carbon, nitrogen, and energy. Using ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mas...
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| Format: | Article |
| Language: | English |
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National Institute of Agronomic Research "INRA" Morocco
2024-12-01
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| Series: | African and Mediterranean Agricultural Journal - Al Awamia |
| Online Access: | https://revues.imist.ma/index.php/Afrimed/article/view/48941 |
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| author | Khaoula Habbadi Hajar Hamzaoui Imane Hourmatallah Hiba Yahyaoui Faiçal Aoujil Majida Hafidi Aziz Aziz Salma El Iraqui El Houssaini |
| author_facet | Khaoula Habbadi Hajar Hamzaoui Imane Hourmatallah Hiba Yahyaoui Faiçal Aoujil Majida Hafidi Aziz Aziz Salma El Iraqui El Houssaini |
| author_sort | Khaoula Habbadi |
| collection | DOAJ |
| description | This study investigates the physiological and genetic diversity of Allorhizobium vitis strains in Morocco, focusing on their ability to produce and utilize opines as sources of carbon, nitrogen, and energy. Using ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF) and specific PCR assays, we identified and characterized opines in tumors induced in tomato plants by Moroccan strains of A. vitis. All strains showed the rare octopine/vitopine combination. Chromatographic analysis and molecular validation revealed metabolic and genetic uniformity in opine catabolism among the strains. These results suggest a homogeneity of octopine/vitopine tumour-inducing plasmids (pTi), which contribute to the uniformity of opine-related genes. This comprehensive approach provides insights into the molecular mechanisms of opine utilization and bacterial virulence, suggesting that targeting specific pathways or key virulence genes may be a strategy to control crown gall disease. |
| format | Article |
| id | doaj-art-16b0c30b9c4b47a2a68264d011b794f6 |
| institution | Kabale University |
| issn | 0572-2721 2658-9184 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | National Institute of Agronomic Research "INRA" Morocco |
| record_format | Article |
| series | African and Mediterranean Agricultural Journal - Al Awamia |
| spelling | doaj-art-16b0c30b9c4b47a2a68264d011b794f62025-01-07T14:58:12ZengNational Institute of Agronomic Research "INRA" MoroccoAfrican and Mediterranean Agricultural Journal - Al Awamia0572-27212658-91842024-12-0114516317310.34874/IMIST.PRSM/afrimed-i145.4894168706Characterizing Opine Physiological and Genetic Diversity in Moroccan strains of Allorhizobium vitis, the Causal Agent of Grapevine Crown GallKhaoula Habbadi0https://orcid.org/0000-0002-1890-4601Hajar Hamzaoui1Imane Hourmatallah2Hiba Yahyaoui3Faiçal Aoujil4Majida Hafidi5Aziz Aziz6Salma El Iraqui El Houssaini7INRA, Regional Center of Agricultural Research of MeknesSidi Mohamed Ben Abdellah University, Faculté of Sciences of Fes & INRA Regional Center of Agricultural Research of MeknsSidi Mohamed Ben Abdellah University, Faculté of Sciences of Fes & INRA Regional Center of Agricultural Research of MeknsMoulay Ismail University, Faculty of Sciences of Meknes & INRA Regional Center of Agricultural Research of MeknesMoulay Ismail University, Faculty of Sciences of Meknes & INRA Regional Center of Agricultural Research of MeknesMoulay Ismail University, Faculty of Sciences of MeknesReims Champagne-Ardenne UniversityINRA, Regional Center of Agricultural Research of MeknesThis study investigates the physiological and genetic diversity of Allorhizobium vitis strains in Morocco, focusing on their ability to produce and utilize opines as sources of carbon, nitrogen, and energy. Using ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF) and specific PCR assays, we identified and characterized opines in tumors induced in tomato plants by Moroccan strains of A. vitis. All strains showed the rare octopine/vitopine combination. Chromatographic analysis and molecular validation revealed metabolic and genetic uniformity in opine catabolism among the strains. These results suggest a homogeneity of octopine/vitopine tumour-inducing plasmids (pTi), which contribute to the uniformity of opine-related genes. This comprehensive approach provides insights into the molecular mechanisms of opine utilization and bacterial virulence, suggesting that targeting specific pathways or key virulence genes may be a strategy to control crown gall disease.https://revues.imist.ma/index.php/Afrimed/article/view/48941 |
| spellingShingle | Khaoula Habbadi Hajar Hamzaoui Imane Hourmatallah Hiba Yahyaoui Faiçal Aoujil Majida Hafidi Aziz Aziz Salma El Iraqui El Houssaini Characterizing Opine Physiological and Genetic Diversity in Moroccan strains of Allorhizobium vitis, the Causal Agent of Grapevine Crown Gall African and Mediterranean Agricultural Journal - Al Awamia |
| title | Characterizing Opine Physiological and Genetic Diversity in Moroccan strains of Allorhizobium vitis, the Causal Agent of Grapevine Crown Gall |
| title_full | Characterizing Opine Physiological and Genetic Diversity in Moroccan strains of Allorhizobium vitis, the Causal Agent of Grapevine Crown Gall |
| title_fullStr | Characterizing Opine Physiological and Genetic Diversity in Moroccan strains of Allorhizobium vitis, the Causal Agent of Grapevine Crown Gall |
| title_full_unstemmed | Characterizing Opine Physiological and Genetic Diversity in Moroccan strains of Allorhizobium vitis, the Causal Agent of Grapevine Crown Gall |
| title_short | Characterizing Opine Physiological and Genetic Diversity in Moroccan strains of Allorhizobium vitis, the Causal Agent of Grapevine Crown Gall |
| title_sort | characterizing opine physiological and genetic diversity in moroccan strains of allorhizobium vitis the causal agent of grapevine crown gall |
| url | https://revues.imist.ma/index.php/Afrimed/article/view/48941 |
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