Paradigm of engineering recalcitrant non-model microorganism with dominant metabolic pathway as a biorefinery chassis
Abstract The development and implementation of microbial chassis cells have profound impacts on circular economy. Non-model bacterium Zymomonas mobilis is an excellent chassis owing to its extraordinary industrial characteristics. Here, the genome-scale metabolic model iZM516 is improved and updated...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-54897-5 |
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| author | Xiongying Yan Weiwei Bao Yalun Wu Chenyue Zhang Zhitao Mao Qianqian Yuan Zhousheng Hu Penghui He Qiqun Peng Mimi Hu Binan Geng Hongwu Ma Shouwen Chen Qiang Fei Qiaoning He Shihui Yang |
| author_facet | Xiongying Yan Weiwei Bao Yalun Wu Chenyue Zhang Zhitao Mao Qianqian Yuan Zhousheng Hu Penghui He Qiqun Peng Mimi Hu Binan Geng Hongwu Ma Shouwen Chen Qiang Fei Qiaoning He Shihui Yang |
| author_sort | Xiongying Yan |
| collection | DOAJ |
| description | Abstract The development and implementation of microbial chassis cells have profound impacts on circular economy. Non-model bacterium Zymomonas mobilis is an excellent chassis owing to its extraordinary industrial characteristics. Here, the genome-scale metabolic model iZM516 is improved and updated by integrating enzyme constraints to simulate the dynamics of flux distribution and guide pathway design. We show that the innate dominant ethanol pathway of Z. mobilis restricts the titer and rate of these biochemicals. A dominant-metabolism compromised intermediate-chassis (DMCI) strategy is then developed through introducing low toxicity but cofactor imbalanced 2,3-butanediol pathway, and a recombinant D-lactate producer is constructed to produce more than 140.92 g/L and 104.6 g/L D-lactate (yield > 0.97 g/g) from glucose and corncob residue hydrolysate, respectively. Additionally, techno-economic analysis (TEA) and life cycle assessment (LCA) demonstrate the commercialization feasibility and greenhouse gas reduction capability of lignocellulosic D-lactate. This work thus establishes a paradigm for engineering recalcitrant microorganisms as biorefinery chassis. |
| format | Article |
| id | doaj-art-169ffd0e773c4e6b9297a1550bde0d65 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-169ffd0e773c4e6b9297a1550bde0d652024-12-01T12:35:07ZengNature PortfolioNature Communications2041-17232024-11-0115111510.1038/s41467-024-54897-5Paradigm of engineering recalcitrant non-model microorganism with dominant metabolic pathway as a biorefinery chassisXiongying Yan0Weiwei Bao1Yalun Wu2Chenyue Zhang3Zhitao Mao4Qianqian Yuan5Zhousheng Hu6Penghui He7Qiqun Peng8Mimi Hu9Binan Geng10Hongwu Ma11Shouwen Chen12Qiang Fei13Qiaoning He14Shihui Yang15State Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, and School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, and School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, and School of Life Sciences, Hubei UniversityXi’an Key Laboratory of C1 Compound Bioconversion Technology, School of Chemical Engineering and Technology, Xi’an Jiaotong UniversityBiodesign Center, Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesBiodesign Center, Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesState Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, and School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, and School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, and School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, and School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, and School of Life Sciences, Hubei UniversityBiodesign Center, Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesState Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, and School of Life Sciences, Hubei UniversityXi’an Key Laboratory of C1 Compound Bioconversion Technology, School of Chemical Engineering and Technology, Xi’an Jiaotong UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, and School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, and School of Life Sciences, Hubei UniversityAbstract The development and implementation of microbial chassis cells have profound impacts on circular economy. Non-model bacterium Zymomonas mobilis is an excellent chassis owing to its extraordinary industrial characteristics. Here, the genome-scale metabolic model iZM516 is improved and updated by integrating enzyme constraints to simulate the dynamics of flux distribution and guide pathway design. We show that the innate dominant ethanol pathway of Z. mobilis restricts the titer and rate of these biochemicals. A dominant-metabolism compromised intermediate-chassis (DMCI) strategy is then developed through introducing low toxicity but cofactor imbalanced 2,3-butanediol pathway, and a recombinant D-lactate producer is constructed to produce more than 140.92 g/L and 104.6 g/L D-lactate (yield > 0.97 g/g) from glucose and corncob residue hydrolysate, respectively. Additionally, techno-economic analysis (TEA) and life cycle assessment (LCA) demonstrate the commercialization feasibility and greenhouse gas reduction capability of lignocellulosic D-lactate. This work thus establishes a paradigm for engineering recalcitrant microorganisms as biorefinery chassis.https://doi.org/10.1038/s41467-024-54897-5 |
| spellingShingle | Xiongying Yan Weiwei Bao Yalun Wu Chenyue Zhang Zhitao Mao Qianqian Yuan Zhousheng Hu Penghui He Qiqun Peng Mimi Hu Binan Geng Hongwu Ma Shouwen Chen Qiang Fei Qiaoning He Shihui Yang Paradigm of engineering recalcitrant non-model microorganism with dominant metabolic pathway as a biorefinery chassis Nature Communications |
| title | Paradigm of engineering recalcitrant non-model microorganism with dominant metabolic pathway as a biorefinery chassis |
| title_full | Paradigm of engineering recalcitrant non-model microorganism with dominant metabolic pathway as a biorefinery chassis |
| title_fullStr | Paradigm of engineering recalcitrant non-model microorganism with dominant metabolic pathway as a biorefinery chassis |
| title_full_unstemmed | Paradigm of engineering recalcitrant non-model microorganism with dominant metabolic pathway as a biorefinery chassis |
| title_short | Paradigm of engineering recalcitrant non-model microorganism with dominant metabolic pathway as a biorefinery chassis |
| title_sort | paradigm of engineering recalcitrant non model microorganism with dominant metabolic pathway as a biorefinery chassis |
| url | https://doi.org/10.1038/s41467-024-54897-5 |
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