OZONE IMPROVES AUTOGENOUS GRAFT HEALING IN EXPERIMENTAL DIABETES MELLITUS: A MORPHOMETRIC AND IMMUNOHISTOCHEMICAL STUDY
Backround: Diabetes mellitus (DM) is a metabolic disorder which plays crucial role in the pathogenesis of periodontitis. Ozone have several actions such as antimicrobial ,immunostimulating, antihypoxic effects, and activates antioxidant enzymes and angiogenesisAim: The aim of this study is to invest...
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| Format: | Article |
| Language: | English |
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Selcuk University Press
2020-04-01
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| Series: | Selcuk Dental Journal |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/1029627 |
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| author | Aysan Lektemür Alpan Hülya Toker Hatice Özer |
| author_facet | Aysan Lektemür Alpan Hülya Toker Hatice Özer |
| author_sort | Aysan Lektemür Alpan |
| collection | DOAJ |
| description | Backround: Diabetes
mellitus (DM) is a metabolic disorder which plays crucial role in the pathogenesis of periodontitis. Ozone
have several actions such as
antimicrobial ,immunostimulating, antihypoxic effects, and activates
antioxidant enzymes and angiogenesisAim:
The aim of this study is to investigate the
effect of gaseous ozone on bone healing in diabetic rat calvarial defects
treated with autogenous bone graft, morphometrically and immunohistochemically.Material and Methods:
Diabetes was induced and critical size defects were created on rats. Study
groups: 1-Empty defect (Control, n=14) group, 2-Autograft (AG, n=14) group,
3-Empty defect+ozone therapy (Control+Ozone, n=14) group, 4-Autograft+ozone
application (AG+Ozone, n=14) group. Gaseous ozone was applied on the operation
day and the following 2 weeks daily (140ppm @
2L/d, 2.24 mg). Total bone area was measured. Osteocalcin and Bone morphogenic
protein-2 protein expressions were evaluated.Results: Control and Control+Ozone groups had no osteoclast and
residuel lacunae during the study . Osteoblasts in AG+Ozone group were higher
than AG group at 4th week (p>0.05). AG+Ozone group had more total bone area
than AG group at 4th week. AG+Ozone group revealed more BMP-2 immune positivity
compared to the other groups. Osteocalcin immune positivity in AG groups was
higher than those of the Control groups.Conclusion: Within the limitations of this study, gaseous ozone application
decreased osteoclast number and increased osteoblast number and bone
regeneration, especially, in early stages of bone regeneration in diabetic
rats.
KEYWORDS: autograft, bone regeneration, calvarial defect, experimental
diabetes mellitus, ozone |
| format | Article |
| id | doaj-art-168aa5a50f834ab5b70409731f81141f |
| institution | Kabale University |
| issn | 2148-7529 |
| language | English |
| publishDate | 2020-04-01 |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Selcuk Dental Journal |
| spelling | doaj-art-168aa5a50f834ab5b70409731f81141f2025-01-03T01:32:54ZengSelcuk University PressSelcuk Dental Journal2148-75292020-04-0171445310.15311/selcukdentj.419582154OZONE IMPROVES AUTOGENOUS GRAFT HEALING IN EXPERIMENTAL DIABETES MELLITUS: A MORPHOMETRIC AND IMMUNOHISTOCHEMICAL STUDYAysan Lektemür Alpan0Hülya Toker1Hatice Özer2Denizli Pamukkale UniversityCumhuriyet Üniversitesi | Cumhuriyet UniversityCumhuriyet Üniversitesi | Cumhuriyet UniversityBackround: Diabetes mellitus (DM) is a metabolic disorder which plays crucial role in the pathogenesis of periodontitis. Ozone have several actions such as antimicrobial ,immunostimulating, antihypoxic effects, and activates antioxidant enzymes and angiogenesisAim: The aim of this study is to investigate the effect of gaseous ozone on bone healing in diabetic rat calvarial defects treated with autogenous bone graft, morphometrically and immunohistochemically.Material and Methods: Diabetes was induced and critical size defects were created on rats. Study groups: 1-Empty defect (Control, n=14) group, 2-Autograft (AG, n=14) group, 3-Empty defect+ozone therapy (Control+Ozone, n=14) group, 4-Autograft+ozone application (AG+Ozone, n=14) group. Gaseous ozone was applied on the operation day and the following 2 weeks daily (140ppm @ 2L/d, 2.24 mg). Total bone area was measured. Osteocalcin and Bone morphogenic protein-2 protein expressions were evaluated.Results: Control and Control+Ozone groups had no osteoclast and residuel lacunae during the study . Osteoblasts in AG+Ozone group were higher than AG group at 4th week (p>0.05). AG+Ozone group had more total bone area than AG group at 4th week. AG+Ozone group revealed more BMP-2 immune positivity compared to the other groups. Osteocalcin immune positivity in AG groups was higher than those of the Control groups.Conclusion: Within the limitations of this study, gaseous ozone application decreased osteoclast number and increased osteoblast number and bone regeneration, especially, in early stages of bone regeneration in diabetic rats. KEYWORDS: autograft, bone regeneration, calvarial defect, experimental diabetes mellitus, ozonehttps://dergipark.org.tr/tr/download/article-file/1029627autograftbone regenerationcalvarial defectexperimental diabetes mellitus: autograftbone regenerationcalvarial defectexperimental diabetes mellitus |
| spellingShingle | Aysan Lektemür Alpan Hülya Toker Hatice Özer OZONE IMPROVES AUTOGENOUS GRAFT HEALING IN EXPERIMENTAL DIABETES MELLITUS: A MORPHOMETRIC AND IMMUNOHISTOCHEMICAL STUDY Selcuk Dental Journal autograft bone regeneration calvarial defect experimental diabetes mellitus : autograft bone regeneration calvarial defect experimental diabetes mellitus |
| title | OZONE IMPROVES AUTOGENOUS GRAFT HEALING IN EXPERIMENTAL DIABETES MELLITUS: A MORPHOMETRIC AND IMMUNOHISTOCHEMICAL STUDY |
| title_full | OZONE IMPROVES AUTOGENOUS GRAFT HEALING IN EXPERIMENTAL DIABETES MELLITUS: A MORPHOMETRIC AND IMMUNOHISTOCHEMICAL STUDY |
| title_fullStr | OZONE IMPROVES AUTOGENOUS GRAFT HEALING IN EXPERIMENTAL DIABETES MELLITUS: A MORPHOMETRIC AND IMMUNOHISTOCHEMICAL STUDY |
| title_full_unstemmed | OZONE IMPROVES AUTOGENOUS GRAFT HEALING IN EXPERIMENTAL DIABETES MELLITUS: A MORPHOMETRIC AND IMMUNOHISTOCHEMICAL STUDY |
| title_short | OZONE IMPROVES AUTOGENOUS GRAFT HEALING IN EXPERIMENTAL DIABETES MELLITUS: A MORPHOMETRIC AND IMMUNOHISTOCHEMICAL STUDY |
| title_sort | ozone improves autogenous graft healing in experimental diabetes mellitus a morphometric and immunohistochemical study |
| topic | autograft bone regeneration calvarial defect experimental diabetes mellitus : autograft bone regeneration calvarial defect experimental diabetes mellitus |
| url | https://dergipark.org.tr/tr/download/article-file/1029627 |
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