Genome-Wide Identification and Expression Analysis of Amino Acid/Auxin Permease (AAAP) Genes in Grapes (<i>Vitis vinifera</i> L.) Under Abiotic Stress and During Development
Amino acids in wine grapes function as precursors for various secondary metabolites and play a vital role in plant growth, development, and stress resistance. The amino acid/auxin permease (<i>AAAP</i>) genes encode a large family of transporters; however, the identification and function...
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2025-01-01
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| author | Xufeng Guo Na He Biying Huang Chongyao Chen Yanxia Zhang Xiaoyu Yang Jie Li Zhigang Dong |
| author_facet | Xufeng Guo Na He Biying Huang Chongyao Chen Yanxia Zhang Xiaoyu Yang Jie Li Zhigang Dong |
| author_sort | Xufeng Guo |
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| description | Amino acids in wine grapes function as precursors for various secondary metabolites and play a vital role in plant growth, development, and stress resistance. The amino acid/auxin permease (<i>AAAP</i>) genes encode a large family of transporters; however, the identification and function of the <i>AAAP</i> gene family in grapes remain limited. Consequently, we conducted a comprehensive bioinformatics analysis of all <i>AAAP</i> genes in grapes, encompassing genome sequence analysis, conserved protein domain identification, chromosomal localization, phylogenetic relationship analysis, and gene expression profiling. This study identified 60 <i>VvAAAP</i> genes, distributed on 14 chromosomes and classified into eight subfamilies. Microarray and transcriptome data revealed that most <i>VvAAAP</i> genes decrease during development, but <i>VvAAAP7</i> and <i>VvAAAP33</i> gradually increase. <i>VvAAAP23</i> and <i>VvAAAP46</i> exhibited significantly higher expression levels, while <i>VvAAAP30</i> demonstrated lower expression when subjected to salt and drought stress. <i>VvAAAP</i> genes exhibited diverse expression patterns, suggesting that the <i>AAAP</i> gene family possesses both diversity and specific functions in grapes. Furthermore, the expression patterns of <i>VvAAAP</i> genes analyzed by RT-qPCR facilitate further investigation into the biological functions of individual genes in different tissues. These findings provide valuable insights into the continued analysis of the <i>AAAP</i> gene family’s functions in grapes. |
| format | Article |
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| institution | Kabale University |
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| publishDate | 2025-01-01 |
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| spelling | doaj-art-1643efd94d3f4704bda8ccb4da4bde302025-01-10T13:19:50ZengMDPI AGPlants2223-77472025-01-0114112810.3390/plants14010128Genome-Wide Identification and Expression Analysis of Amino Acid/Auxin Permease (AAAP) Genes in Grapes (<i>Vitis vinifera</i> L.) Under Abiotic Stress and During DevelopmentXufeng Guo0Na He1Biying Huang2Chongyao Chen3Yanxia Zhang4Xiaoyu Yang5Jie Li6Zhigang Dong7College of Horticulture, Pomology Institute, Shanxi Agricultural University, Jinzhong 030800, ChinaCollege of Horticulture, Pomology Institute, Shanxi Agricultural University, Jinzhong 030800, ChinaCollege of Horticulture, Pomology Institute, Shanxi Agricultural University, Jinzhong 030800, ChinaCollege of Horticulture, Pomology Institute, Shanxi Agricultural University, Jinzhong 030800, ChinaCollege of Horticulture, Pomology Institute, Shanxi Agricultural University, Jinzhong 030800, ChinaCollege of Horticulture, Pomology Institute, Shanxi Agricultural University, Jinzhong 030800, ChinaCollege of Horticulture, Pomology Institute, Shanxi Agricultural University, Jinzhong 030800, ChinaCollege of Horticulture, Pomology Institute, Shanxi Agricultural University, Jinzhong 030800, ChinaAmino acids in wine grapes function as precursors for various secondary metabolites and play a vital role in plant growth, development, and stress resistance. The amino acid/auxin permease (<i>AAAP</i>) genes encode a large family of transporters; however, the identification and function of the <i>AAAP</i> gene family in grapes remain limited. Consequently, we conducted a comprehensive bioinformatics analysis of all <i>AAAP</i> genes in grapes, encompassing genome sequence analysis, conserved protein domain identification, chromosomal localization, phylogenetic relationship analysis, and gene expression profiling. This study identified 60 <i>VvAAAP</i> genes, distributed on 14 chromosomes and classified into eight subfamilies. Microarray and transcriptome data revealed that most <i>VvAAAP</i> genes decrease during development, but <i>VvAAAP7</i> and <i>VvAAAP33</i> gradually increase. <i>VvAAAP23</i> and <i>VvAAAP46</i> exhibited significantly higher expression levels, while <i>VvAAAP30</i> demonstrated lower expression when subjected to salt and drought stress. <i>VvAAAP</i> genes exhibited diverse expression patterns, suggesting that the <i>AAAP</i> gene family possesses both diversity and specific functions in grapes. Furthermore, the expression patterns of <i>VvAAAP</i> genes analyzed by RT-qPCR facilitate further investigation into the biological functions of individual genes in different tissues. These findings provide valuable insights into the continued analysis of the <i>AAAP</i> gene family’s functions in grapes.https://www.mdpi.com/2223-7747/14/1/128<i>Vitis vinifera</i>. L.amino acid transportcollinearity analysisabiotic stressdevelopment |
| spellingShingle | Xufeng Guo Na He Biying Huang Chongyao Chen Yanxia Zhang Xiaoyu Yang Jie Li Zhigang Dong Genome-Wide Identification and Expression Analysis of Amino Acid/Auxin Permease (AAAP) Genes in Grapes (<i>Vitis vinifera</i> L.) Under Abiotic Stress and During Development Plants <i>Vitis vinifera</i>. L. amino acid transport collinearity analysis abiotic stress development |
| title | Genome-Wide Identification and Expression Analysis of Amino Acid/Auxin Permease (AAAP) Genes in Grapes (<i>Vitis vinifera</i> L.) Under Abiotic Stress and During Development |
| title_full | Genome-Wide Identification and Expression Analysis of Amino Acid/Auxin Permease (AAAP) Genes in Grapes (<i>Vitis vinifera</i> L.) Under Abiotic Stress and During Development |
| title_fullStr | Genome-Wide Identification and Expression Analysis of Amino Acid/Auxin Permease (AAAP) Genes in Grapes (<i>Vitis vinifera</i> L.) Under Abiotic Stress and During Development |
| title_full_unstemmed | Genome-Wide Identification and Expression Analysis of Amino Acid/Auxin Permease (AAAP) Genes in Grapes (<i>Vitis vinifera</i> L.) Under Abiotic Stress and During Development |
| title_short | Genome-Wide Identification and Expression Analysis of Amino Acid/Auxin Permease (AAAP) Genes in Grapes (<i>Vitis vinifera</i> L.) Under Abiotic Stress and During Development |
| title_sort | genome wide identification and expression analysis of amino acid auxin permease aaap genes in grapes i vitis vinifera i l under abiotic stress and during development |
| topic | <i>Vitis vinifera</i>. L. amino acid transport collinearity analysis abiotic stress development |
| url | https://www.mdpi.com/2223-7747/14/1/128 |
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