Genome-Wide Identification and Expression Analysis of Amino Acid/Auxin Permease (AAAP) Genes in Grapes (<i>Vitis vinifera</i> L.) Under Abiotic Stress and During Development
Amino acids in wine grapes function as precursors for various secondary metabolites and play a vital role in plant growth, development, and stress resistance. The amino acid/auxin permease (<i>AAAP</i>) genes encode a large family of transporters; however, the identification and function...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/14/1/128 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Amino acids in wine grapes function as precursors for various secondary metabolites and play a vital role in plant growth, development, and stress resistance. The amino acid/auxin permease (<i>AAAP</i>) genes encode a large family of transporters; however, the identification and function of the <i>AAAP</i> gene family in grapes remain limited. Consequently, we conducted a comprehensive bioinformatics analysis of all <i>AAAP</i> genes in grapes, encompassing genome sequence analysis, conserved protein domain identification, chromosomal localization, phylogenetic relationship analysis, and gene expression profiling. This study identified 60 <i>VvAAAP</i> genes, distributed on 14 chromosomes and classified into eight subfamilies. Microarray and transcriptome data revealed that most <i>VvAAAP</i> genes decrease during development, but <i>VvAAAP7</i> and <i>VvAAAP33</i> gradually increase. <i>VvAAAP23</i> and <i>VvAAAP46</i> exhibited significantly higher expression levels, while <i>VvAAAP30</i> demonstrated lower expression when subjected to salt and drought stress. <i>VvAAAP</i> genes exhibited diverse expression patterns, suggesting that the <i>AAAP</i> gene family possesses both diversity and specific functions in grapes. Furthermore, the expression patterns of <i>VvAAAP</i> genes analyzed by RT-qPCR facilitate further investigation into the biological functions of individual genes in different tissues. These findings provide valuable insights into the continued analysis of the <i>AAAP</i> gene family’s functions in grapes. |
|---|---|
| ISSN: | 2223-7747 |