Protocol for differentiating hematopoietic progenitor cells from human pluripotent stem cells in chemically defined monolayer culture
Summary: Human pluripotent stem cells (hPSCs) provide a powerful platform for generating hematopoietic progenitor cells (HPCs) and investigating hematopoietic development. Here, we present a protocol for maintaining hPSCs and inducing their differentiation into HPCs through the endothelial-to-hemato...
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| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672400710X |
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| author | Shaokang Mo Kengyuan Qu Jun Shen Kuangyu Yen |
| author_facet | Shaokang Mo Kengyuan Qu Jun Shen Kuangyu Yen |
| author_sort | Shaokang Mo |
| collection | DOAJ |
| description | Summary: Human pluripotent stem cells (hPSCs) provide a powerful platform for generating hematopoietic progenitor cells (HPCs) and investigating hematopoietic development. Here, we present a protocol for maintaining hPSCs and inducing their differentiation into HPCs through the endothelial-to-hematopoietic transition (EHT) on vitronectin-coated plates. We outline steps for evaluating the efficiency of HPC generation and assessing their potential to differentiate into various hematopoietic lineages. This protocol serves as a framework for exploring human hematopoiesis and generating various functional blood cells.For complete details on the use and execution of this protocol, please refer to Shen et al.1 and Qu et al.2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-15db1a494dac46d4bc3dd0941ede4f2a |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-15db1a494dac46d4bc3dd0941ede4f2a2025-01-13T04:19:11ZengElsevierSTAR Protocols2666-16672025-03-0161103545Protocol for differentiating hematopoietic progenitor cells from human pluripotent stem cells in chemically defined monolayer cultureShaokang Mo0Kengyuan Qu1Jun Shen2Kuangyu Yen3State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, ChinaState Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, ChinaState Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China; Corresponding authorState Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China; Corresponding authorSummary: Human pluripotent stem cells (hPSCs) provide a powerful platform for generating hematopoietic progenitor cells (HPCs) and investigating hematopoietic development. Here, we present a protocol for maintaining hPSCs and inducing their differentiation into HPCs through the endothelial-to-hematopoietic transition (EHT) on vitronectin-coated plates. We outline steps for evaluating the efficiency of HPC generation and assessing their potential to differentiate into various hematopoietic lineages. This protocol serves as a framework for exploring human hematopoiesis and generating various functional blood cells.For complete details on the use and execution of this protocol, please refer to Shen et al.1 and Qu et al.2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S266616672400710Xcell biologycell culturedevelopmental biologystem cellscell differentiation |
| spellingShingle | Shaokang Mo Kengyuan Qu Jun Shen Kuangyu Yen Protocol for differentiating hematopoietic progenitor cells from human pluripotent stem cells in chemically defined monolayer culture STAR Protocols cell biology cell culture developmental biology stem cells cell differentiation |
| title | Protocol for differentiating hematopoietic progenitor cells from human pluripotent stem cells in chemically defined monolayer culture |
| title_full | Protocol for differentiating hematopoietic progenitor cells from human pluripotent stem cells in chemically defined monolayer culture |
| title_fullStr | Protocol for differentiating hematopoietic progenitor cells from human pluripotent stem cells in chemically defined monolayer culture |
| title_full_unstemmed | Protocol for differentiating hematopoietic progenitor cells from human pluripotent stem cells in chemically defined monolayer culture |
| title_short | Protocol for differentiating hematopoietic progenitor cells from human pluripotent stem cells in chemically defined monolayer culture |
| title_sort | protocol for differentiating hematopoietic progenitor cells from human pluripotent stem cells in chemically defined monolayer culture |
| topic | cell biology cell culture developmental biology stem cells cell differentiation |
| url | http://www.sciencedirect.com/science/article/pii/S266616672400710X |
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