Fish cells persistently infected with nervous necrosis virus produce a small-molecule substance for reducing cellular metabolism and suppressing viral multiplication
Abstract Nervous necrosis virus (NNV) of the genus Betanodavirus is one of the simplest RNA viruses pathogenic to a wide range of fish species. We established the SeGF, SeGE-22 and SeGB cell lines persistently infected with NNV (PI-SeGFNNV, PI-SeGE-22NNV and PI-SeGBNNV cells) by repeatedly subcultur...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-84751-z |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841559713841414144 |
|---|---|
| author | Han Sol Lee Toyohiko Nishizawa |
| author_facet | Han Sol Lee Toyohiko Nishizawa |
| author_sort | Han Sol Lee |
| collection | DOAJ |
| description | Abstract Nervous necrosis virus (NNV) of the genus Betanodavirus is one of the simplest RNA viruses pathogenic to a wide range of fish species. We established the SeGF, SeGE-22 and SeGB cell lines persistently infected with NNV (PI-SeGFNNV, PI-SeGE-22NNV and PI-SeGBNNV cells) by repeatedly subculturing the cells that survived NNV infection. The PI-SeGFNNV and PI-SeGE-22NNV cells continued to stably yield NNV in culture fluids at 106 to 107 median tissue culture infectious dose (TCID50)/ml even after 30–50 subcultures. The PI-SeGBNNV cells initially yielded NNV at 103.9 TCID50/ml but stopped yielding NNV after several passages. No significant morphological differences were observed between the naïve and PI-cells in either cell line. Antiviral activity suppressing the multiplication of NNV was detected in the culture fluids of all PI-cell lines. It significantly suppressed the growth (metabolism) of each cell line but did not directly influence NNV infectivity. However, this antiviral substance was not an interferon but a heat-stable (100 ºC for 3 min), small molecule with M r < 1000. When the PI-SeGBNNV cells stopped yielding NNV after subculturing several times, the production of the antiviral substance also ceased, indicating that the production of antiviral substance is initiated by NNV infection. |
| format | Article |
| id | doaj-art-15a2ed6ea9964eae9709f7454a227c14 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-15a2ed6ea9964eae9709f7454a227c142025-01-05T12:15:29ZengNature PortfolioScientific Reports2045-23222025-01-0115111110.1038/s41598-024-84751-zFish cells persistently infected with nervous necrosis virus produce a small-molecule substance for reducing cellular metabolism and suppressing viral multiplicationHan Sol Lee0Toyohiko Nishizawa1Department of Aqualife Medicine, Chonnam National UniversityDepartment of Aqualife Medicine, Chonnam National UniversityAbstract Nervous necrosis virus (NNV) of the genus Betanodavirus is one of the simplest RNA viruses pathogenic to a wide range of fish species. We established the SeGF, SeGE-22 and SeGB cell lines persistently infected with NNV (PI-SeGFNNV, PI-SeGE-22NNV and PI-SeGBNNV cells) by repeatedly subculturing the cells that survived NNV infection. The PI-SeGFNNV and PI-SeGE-22NNV cells continued to stably yield NNV in culture fluids at 106 to 107 median tissue culture infectious dose (TCID50)/ml even after 30–50 subcultures. The PI-SeGBNNV cells initially yielded NNV at 103.9 TCID50/ml but stopped yielding NNV after several passages. No significant morphological differences were observed between the naïve and PI-cells in either cell line. Antiviral activity suppressing the multiplication of NNV was detected in the culture fluids of all PI-cell lines. It significantly suppressed the growth (metabolism) of each cell line but did not directly influence NNV infectivity. However, this antiviral substance was not an interferon but a heat-stable (100 ºC for 3 min), small molecule with M r < 1000. When the PI-SeGBNNV cells stopped yielding NNV after subculturing several times, the production of the antiviral substance also ceased, indicating that the production of antiviral substance is initiated by NNV infection.https://doi.org/10.1038/s41598-024-84751-zNervous necrosis virus (NNV)Persistent infectionAntiviral substanceInterferonSevenband grouper cell linesFish disease |
| spellingShingle | Han Sol Lee Toyohiko Nishizawa Fish cells persistently infected with nervous necrosis virus produce a small-molecule substance for reducing cellular metabolism and suppressing viral multiplication Scientific Reports Nervous necrosis virus (NNV) Persistent infection Antiviral substance Interferon Sevenband grouper cell lines Fish disease |
| title | Fish cells persistently infected with nervous necrosis virus produce a small-molecule substance for reducing cellular metabolism and suppressing viral multiplication |
| title_full | Fish cells persistently infected with nervous necrosis virus produce a small-molecule substance for reducing cellular metabolism and suppressing viral multiplication |
| title_fullStr | Fish cells persistently infected with nervous necrosis virus produce a small-molecule substance for reducing cellular metabolism and suppressing viral multiplication |
| title_full_unstemmed | Fish cells persistently infected with nervous necrosis virus produce a small-molecule substance for reducing cellular metabolism and suppressing viral multiplication |
| title_short | Fish cells persistently infected with nervous necrosis virus produce a small-molecule substance for reducing cellular metabolism and suppressing viral multiplication |
| title_sort | fish cells persistently infected with nervous necrosis virus produce a small molecule substance for reducing cellular metabolism and suppressing viral multiplication |
| topic | Nervous necrosis virus (NNV) Persistent infection Antiviral substance Interferon Sevenband grouper cell lines Fish disease |
| url | https://doi.org/10.1038/s41598-024-84751-z |
| work_keys_str_mv | AT hansollee fishcellspersistentlyinfectedwithnervousnecrosisvirusproduceasmallmoleculesubstanceforreducingcellularmetabolismandsuppressingviralmultiplication AT toyohikonishizawa fishcellspersistentlyinfectedwithnervousnecrosisvirusproduceasmallmoleculesubstanceforreducingcellularmetabolismandsuppressingviralmultiplication |