An Isoflavone Synthase Gene in <i>Arachis hypogea</i> Responds to <i>Phoma arachidicola</i> Infection Causing Web Blotch
Peanut web blotch is an important leaf disease caused by <i>Phoma arachidicola</i>, which seriously affects the quality and yield of peanuts. However, the molecular mechanisms of peanut resistance to peanut web blotch are not well understood. In this study, a transcriptome analysis of th...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-10-01
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| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/13/21/2948 |
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| Summary: | Peanut web blotch is an important leaf disease caused by <i>Phoma arachidicola</i>, which seriously affects the quality and yield of peanuts. However, the molecular mechanisms of peanut resistance to peanut web blotch are not well understood. In this study, a transcriptome analysis of the interaction between peanut (<i>Arachis hypogaea</i>) and <i>P. arachidicola</i> revealed that total 2989 (779 up- and 2210 down-regulated) genes were all differentially expressed in peanut leaves infected by <i>P. arachidicola</i> at 7, 14, 21 days post inoculation. The pathways that were strongly differentially expressed were the flavone or isoflavone biosynthesis pathways. In addition, two 2-hydroxy isoflavanone synthase genes, IFS1 and IFS2, were strongly induced by <i>P. arachidicola</i> infection. Overexpression of the two genes enhanced resistance to <i>Phytophthora parasitica</i> in <i>Nicotiana benthamiana</i>. Knockout of <i>AhIFS</i> genes in peanut reduced disease resistance to <i>P. arachidicola</i>. These findings demonstrated that <i>AhIFS</i> genes play key roles in peanut resistance to <i>P. arachidicola</i> infection. Promoter analysis of the two <i>AhIFS</i> genes showed several defense-related cis-elements distributed in the promoter region. This study improves our understanding of the molecular mechanisms behind resistance of peanut infection by <i>P. arachidicola</i>, and provides important information that could be used to undertake greater detailed characterization of web blotch resistance genes in peanut. |
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| ISSN: | 2223-7747 |