Exploring the anticancer and antioxidant potential of gold nanoparticles synthesized from Pterocarpus marsupium bark extract against oral squamous cell carcinoma
Oral squamous cell carcinoma (OSCC) is a disease of significant concern with higher mortality rates. Conventional treatment approaches have several drawbacks, leading to the opening of new research avenues in the field of nanoparticle-based cancer therapeutics. The study aimed at the synthesis of go...
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Taylor & Francis Group
2024-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2024.2416951 |
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| author | Smrutipragnya Samal Rajesh Kumar Meher Pratyush Kumar Das Santosh Kumar Swain Debasmita Dubey Mohd Shahnawaz Khan Bigyan Ranjan Jali |
| author_facet | Smrutipragnya Samal Rajesh Kumar Meher Pratyush Kumar Das Santosh Kumar Swain Debasmita Dubey Mohd Shahnawaz Khan Bigyan Ranjan Jali |
| author_sort | Smrutipragnya Samal |
| collection | DOAJ |
| description | Oral squamous cell carcinoma (OSCC) is a disease of significant concern with higher mortality rates. Conventional treatment approaches have several drawbacks, leading to the opening of new research avenues in the field of nanoparticle-based cancer therapeutics. The study aimed at the synthesis of gold nanoparticles (Pm-AuNPs) from the aqueous bark extract of Pterocarpus marsupium, followed by its characterization and in vitro anticancer evaluation against OSCC. The synthesized Pm-AuNPs were characterized using UV–visible spectroscopy, particle size analyser, zeta potential, FTIR and SEM techniques. The anticancer potential of the Pm-AuNPs was evaluated against OSCC cell lines (SCC29b, SSC154 and OECM-1) through in vitro assays. The IC50 value was found to be 25 ± 1.2, 45 ± 1.5 and 75 ± 2.1 µg/mL for the three OSCC cell lines, elucidating Pm-AuNPs cytotoxic effects and mechanism of action. Intracellular ROS and SOX detection, mitochondrial transmembrane potential analysis and apoptosis detection were used to confirm the activity of Pm-AuNPs against OSCC. Acute toxicity studies on Wistar rats confirmed the non-toxic nature of the Pm-AuNPs at a higher dose concentration up to 2000 mg/kg body weight. The findings underscore Pm-AuNPs as promising candidates for future anticancer therapeutics, providing insights into their mechanism of action and therapeutic efficacy against OSCC. |
| format | Article |
| id | doaj-art-157ea493fdec478982dfc95eeaa6100d |
| institution | Kabale University |
| issn | 2169-1401 2169-141X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj-art-157ea493fdec478982dfc95eeaa6100d2024-12-06T10:48:00ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2024-12-0152151252810.1080/21691401.2024.2416951Exploring the anticancer and antioxidant potential of gold nanoparticles synthesized from Pterocarpus marsupium bark extract against oral squamous cell carcinomaSmrutipragnya Samal0Rajesh Kumar Meher1Pratyush Kumar Das2Santosh Kumar Swain3Debasmita Dubey4Mohd Shahnawaz Khan5Bigyan Ranjan Jali6Department of Otorhinolaryngology and Head and Neck Surgery, IMS and SUM Hospital, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar, IndiaDepartment of Biotechnology and Bioinformatics, Sambalpur University, Burla, IndiaDepartment of Phytopharmaceuticals, School of Agricultural and Bio-Engineering (SoABE), Centurion University of Technology and Management, Paralakhemundi, IndiaDepartment of Otorhinolaryngology and Head and Neck Surgery, All India Institute of Medical Sciences, Bhubaneswar, IndiaMedical Research Laboratory, IMS and SUM Hospital, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar, IndiaDepartment of Biochemistry, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Chemistry, Veer Surendra Sai University of Technology, Burla, IndiaOral squamous cell carcinoma (OSCC) is a disease of significant concern with higher mortality rates. Conventional treatment approaches have several drawbacks, leading to the opening of new research avenues in the field of nanoparticle-based cancer therapeutics. The study aimed at the synthesis of gold nanoparticles (Pm-AuNPs) from the aqueous bark extract of Pterocarpus marsupium, followed by its characterization and in vitro anticancer evaluation against OSCC. The synthesized Pm-AuNPs were characterized using UV–visible spectroscopy, particle size analyser, zeta potential, FTIR and SEM techniques. The anticancer potential of the Pm-AuNPs was evaluated against OSCC cell lines (SCC29b, SSC154 and OECM-1) through in vitro assays. The IC50 value was found to be 25 ± 1.2, 45 ± 1.5 and 75 ± 2.1 µg/mL for the three OSCC cell lines, elucidating Pm-AuNPs cytotoxic effects and mechanism of action. Intracellular ROS and SOX detection, mitochondrial transmembrane potential analysis and apoptosis detection were used to confirm the activity of Pm-AuNPs against OSCC. Acute toxicity studies on Wistar rats confirmed the non-toxic nature of the Pm-AuNPs at a higher dose concentration up to 2000 mg/kg body weight. The findings underscore Pm-AuNPs as promising candidates for future anticancer therapeutics, providing insights into their mechanism of action and therapeutic efficacy against OSCC.https://www.tandfonline.com/doi/10.1080/21691401.2024.2416951Anticancer agentantioxidant activitygold nanoparticlesgreen synthesismitochondria transmembrane potentialPterocarpus marsupium |
| spellingShingle | Smrutipragnya Samal Rajesh Kumar Meher Pratyush Kumar Das Santosh Kumar Swain Debasmita Dubey Mohd Shahnawaz Khan Bigyan Ranjan Jali Exploring the anticancer and antioxidant potential of gold nanoparticles synthesized from Pterocarpus marsupium bark extract against oral squamous cell carcinoma Artificial Cells, Nanomedicine, and Biotechnology Anticancer agent antioxidant activity gold nanoparticles green synthesis mitochondria transmembrane potential Pterocarpus marsupium |
| title | Exploring the anticancer and antioxidant potential of gold nanoparticles synthesized from Pterocarpus marsupium bark extract against oral squamous cell carcinoma |
| title_full | Exploring the anticancer and antioxidant potential of gold nanoparticles synthesized from Pterocarpus marsupium bark extract against oral squamous cell carcinoma |
| title_fullStr | Exploring the anticancer and antioxidant potential of gold nanoparticles synthesized from Pterocarpus marsupium bark extract against oral squamous cell carcinoma |
| title_full_unstemmed | Exploring the anticancer and antioxidant potential of gold nanoparticles synthesized from Pterocarpus marsupium bark extract against oral squamous cell carcinoma |
| title_short | Exploring the anticancer and antioxidant potential of gold nanoparticles synthesized from Pterocarpus marsupium bark extract against oral squamous cell carcinoma |
| title_sort | exploring the anticancer and antioxidant potential of gold nanoparticles synthesized from pterocarpus marsupium bark extract against oral squamous cell carcinoma |
| topic | Anticancer agent antioxidant activity gold nanoparticles green synthesis mitochondria transmembrane potential Pterocarpus marsupium |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2024.2416951 |
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