Analysis on Unveiling the Natural Dynamics of Parthenocarpy and Self-Compatibility in Apple Trees

Apple (<i>Malus domestica</i>) is self-incompatible and typically requires cross-pollination for seed and fruit development. Parthenocarpy (fruit development without fertilization) and self-compatibility (fruit set without external pollen) are highly desirable traits in apple breeding, a...

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Main Authors: Rongmei Wu, Xiaoying Chen, Bin Xia, Yujia Yang, Claire Molloy, Ruiling Wang, Hilary S. Ireland, Robert J. Schaffer, Satish Kumar, Jia-Long Yao
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Horticulturae
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Online Access:https://www.mdpi.com/2311-7524/10/12/1261
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author Rongmei Wu
Xiaoying Chen
Bin Xia
Yujia Yang
Claire Molloy
Ruiling Wang
Hilary S. Ireland
Robert J. Schaffer
Satish Kumar
Jia-Long Yao
author_facet Rongmei Wu
Xiaoying Chen
Bin Xia
Yujia Yang
Claire Molloy
Ruiling Wang
Hilary S. Ireland
Robert J. Schaffer
Satish Kumar
Jia-Long Yao
author_sort Rongmei Wu
collection DOAJ
description Apple (<i>Malus domestica</i>) is self-incompatible and typically requires cross-pollination for seed and fruit development. Parthenocarpy (fruit development without fertilization) and self-compatibility (fruit set without external pollen) are highly desirable traits in apple breeding, as they ensure consistent fruit production and quality without cross-pollination. However, apple parthenocarpic and self-compatible accessions have not been available for practical breeding. To identify these accessions, we analysed 436 accessions of <i>Malus domestica</i> and 84 accessions of wild <i>Malus</i> species by assessing fruit production. Flowers were bagged before opening to prevent cross-pollination. If fruit developed from the bagged flowers, it indicated the presence of self-compatibility or parthenocarpy, depending on whether the fruit contained seeds. We observed and scored a range of phenotypic expressions among accessions, from weak to strong in both parthenocarpy and potential self-compatibility. Strong parthenocarpy was observed in 5.95% of wild <i>Malus</i> species accessions and 3.44% of <i>M. domestica</i> accessions. Similarly, strong self-compatibility was exhibited in 5.95% of wild <i>Malus</i> species accessions and 2.75% of <i>M. domestica</i> accessions. Although bagged flowers showed lower fruit set rates than open-pollinated (OP) flowers, fruit size, weight, firmness, and soluble sugar and starch content showed no significant differences between fruits produced from bagged and OP flowers. Furthermore, a genome-wide association study (GWAS) was conducted with a high-throughput SNP array. This analysis identified several genes potentially associated with these traits. This research provides parthenocarpic and self-compatible apple accessions for breeding, which can generate novel cultivars that eliminate the need for cross-pollination or produce seedless fruit without pollination.
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spelling doaj-art-151f3560095a4c0a97b8701a46caf0b92024-12-27T14:29:03ZengMDPI AGHorticulturae2311-75242024-11-011012126110.3390/horticulturae10121261Analysis on Unveiling the Natural Dynamics of Parthenocarpy and Self-Compatibility in Apple TreesRongmei Wu0Xiaoying Chen1Bin Xia2Yujia Yang3Claire Molloy4Ruiling Wang5Hilary S. Ireland6Robert J. Schaffer7Satish Kumar8Jia-Long Yao9The New Zealand Institute for Plant and Food Research Limited, 120 Mt Albert Road, Sandringham, Auckland 1025, New ZealandThe New Zealand Institute for Plant and Food Research Limited, 30 Crosses Road, Havelock North 4130, New ZealandCollege of Landscape Architecture, Northeast Forestry University, Harbin 150040, ChinaCollege of Landscape Architecture, Northeast Forestry University, Harbin 150040, ChinaThe New Zealand Institute for Plant and Food Research Limited, 30 Crosses Road, Havelock North 4130, New ZealandThe New Zealand Institute for Plant and Food Research Limited, 120 Mt Albert Road, Sandringham, Auckland 1025, New ZealandThe New Zealand Institute for Plant and Food Research Limited, 120 Mt Albert Road, Sandringham, Auckland 1025, New ZealandThe New Zealand Institute for Plant and Food Research Limited, 55 Old Mill Road, RD3, Motueka 7198, New ZealandThe New Zealand Institute for Plant and Food Research Limited, 30 Crosses Road, Havelock North 4130, New ZealandThe New Zealand Institute for Plant and Food Research Limited, 120 Mt Albert Road, Sandringham, Auckland 1025, New ZealandApple (<i>Malus domestica</i>) is self-incompatible and typically requires cross-pollination for seed and fruit development. Parthenocarpy (fruit development without fertilization) and self-compatibility (fruit set without external pollen) are highly desirable traits in apple breeding, as they ensure consistent fruit production and quality without cross-pollination. However, apple parthenocarpic and self-compatible accessions have not been available for practical breeding. To identify these accessions, we analysed 436 accessions of <i>Malus domestica</i> and 84 accessions of wild <i>Malus</i> species by assessing fruit production. Flowers were bagged before opening to prevent cross-pollination. If fruit developed from the bagged flowers, it indicated the presence of self-compatibility or parthenocarpy, depending on whether the fruit contained seeds. We observed and scored a range of phenotypic expressions among accessions, from weak to strong in both parthenocarpy and potential self-compatibility. Strong parthenocarpy was observed in 5.95% of wild <i>Malus</i> species accessions and 3.44% of <i>M. domestica</i> accessions. Similarly, strong self-compatibility was exhibited in 5.95% of wild <i>Malus</i> species accessions and 2.75% of <i>M. domestica</i> accessions. Although bagged flowers showed lower fruit set rates than open-pollinated (OP) flowers, fruit size, weight, firmness, and soluble sugar and starch content showed no significant differences between fruits produced from bagged and OP flowers. Furthermore, a genome-wide association study (GWAS) was conducted with a high-throughput SNP array. This analysis identified several genes potentially associated with these traits. This research provides parthenocarpic and self-compatible apple accessions for breeding, which can generate novel cultivars that eliminate the need for cross-pollination or produce seedless fruit without pollination.https://www.mdpi.com/2311-7524/10/12/1261apple breedingbagging flowersfertilisationfruit setgenome-wide association study (GWAS)genetic resources
spellingShingle Rongmei Wu
Xiaoying Chen
Bin Xia
Yujia Yang
Claire Molloy
Ruiling Wang
Hilary S. Ireland
Robert J. Schaffer
Satish Kumar
Jia-Long Yao
Analysis on Unveiling the Natural Dynamics of Parthenocarpy and Self-Compatibility in Apple Trees
Horticulturae
apple breeding
bagging flowers
fertilisation
fruit set
genome-wide association study (GWAS)
genetic resources
title Analysis on Unveiling the Natural Dynamics of Parthenocarpy and Self-Compatibility in Apple Trees
title_full Analysis on Unveiling the Natural Dynamics of Parthenocarpy and Self-Compatibility in Apple Trees
title_fullStr Analysis on Unveiling the Natural Dynamics of Parthenocarpy and Self-Compatibility in Apple Trees
title_full_unstemmed Analysis on Unveiling the Natural Dynamics of Parthenocarpy and Self-Compatibility in Apple Trees
title_short Analysis on Unveiling the Natural Dynamics of Parthenocarpy and Self-Compatibility in Apple Trees
title_sort analysis on unveiling the natural dynamics of parthenocarpy and self compatibility in apple trees
topic apple breeding
bagging flowers
fertilisation
fruit set
genome-wide association study (GWAS)
genetic resources
url https://www.mdpi.com/2311-7524/10/12/1261
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