Recombinant Analogue of Varicella Zoster Virus Glycoprotein E: Cloning, Expression and Studying of AntigEn Properties
We made recombinant antigen GE containing fragment of VZV glycoprotein E (Gly48 - Glu135) fused to E. coli beta-galactosidase and confirmed its antigen specificity by Western blotting and competitive-inhibition enzyme immunoassay (EIA) in comparison with commercial analogues and natural viral antige...
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| Format: | Article |
| Language: | Russian |
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Numikom LLC
2016-02-01
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| Series: | Эпидемиология и вакцинопрофилактика |
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| Online Access: | https://www.epidemvac.ru/jour/article/view/131 |
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| author | G. I. Alatortseva A. V. Sidorov L. N. Nesterenko L. N. Luhverchik I. I. Amiantova V. V. Docenko Phong Pham Huy A. V. Milovanova O. B. Vylivannaya A. C. Kazanova V. V. Lavrov V. V. Zverev |
| author_facet | G. I. Alatortseva A. V. Sidorov L. N. Nesterenko L. N. Luhverchik I. I. Amiantova V. V. Docenko Phong Pham Huy A. V. Milovanova O. B. Vylivannaya A. C. Kazanova V. V. Lavrov V. V. Zverev |
| author_sort | G. I. Alatortseva |
| collection | DOAJ |
| description | We made recombinant antigen GE containing fragment of VZV glycoprotein E (Gly48 - Glu135) fused to E. coli beta-galactosidase and confirmed its antigen specificity by Western blotting and competitive-inhibition enzyme immunoassay (EIA) in comparison with commercial analogues and natural viral antigens. We showed interaction of recombinant GE protein with IgG antibodies from rabbits immunized by vaccine viral strain. GE protein also specifically reacted in ELISA with 66% of sera from zoster patients and 35% of sera from control groups including sera containing antibodies to other herpes viruses, sera from healthy donors, and sera from patients with different forms of intestinal disorders. Consequently, we demonstrated possibility of application of our recombinant GE VZV as antigen for diagnostics and research use. |
| format | Article |
| id | doaj-art-14eaee10c1d94c2c8f7b48a3389b5041 |
| institution | Kabale University |
| issn | 2073-3046 2619-0494 |
| language | Russian |
| publishDate | 2016-02-01 |
| publisher | Numikom LLC |
| record_format | Article |
| series | Эпидемиология и вакцинопрофилактика |
| spelling | doaj-art-14eaee10c1d94c2c8f7b48a3389b50412025-08-20T03:57:18ZrusNumikom LLCЭпидемиология и вакцинопрофилактика2073-30462619-04942016-02-01151778510.31631/2073-3046-2016-15-1-77-85130Recombinant Analogue of Varicella Zoster Virus Glycoprotein E: Cloning, Expression and Studying of AntigEn PropertiesG. I. Alatortseva0A. V. Sidorov1L. N. Nesterenko2L. N. Luhverchik3I. I. Amiantova4V. V. Docenko5Phong Pham Huy6A. V. Milovanova7O. B. Vylivannaya8A. C. Kazanova9V. V. Lavrov10V. V. Zverev11Mechnikov Research Institute for Vaccines and SeraMechnikov Research Institute for Vaccines and SeraMechnikov Research Institute for Vaccines and SeraMechnikov Research Institute for Vaccines and SeraMechnikov Research Institute for Vaccines and SeraMechnikov Research Institute for Vaccines and SeraMechnikov Research Institute for Vaccines and SeraMechnikov Research Institute for Vaccines and SeraState BudgEtary Institution of Public Health«Diagnostic center of laboratory researches»Mechnikov Research Institute for Vaccines and SeraMechnikov Research Institute for Vaccines and Sera; State Educational Institution of Additional Professional Education «Medical Academy of Postgraduate Studies», Ministry of Healthcare of the Russian FederationMechnikov Research Institute for Vaccines and SeraWe made recombinant antigen GE containing fragment of VZV glycoprotein E (Gly48 - Glu135) fused to E. coli beta-galactosidase and confirmed its antigen specificity by Western blotting and competitive-inhibition enzyme immunoassay (EIA) in comparison with commercial analogues and natural viral antigens. We showed interaction of recombinant GE protein with IgG antibodies from rabbits immunized by vaccine viral strain. GE protein also specifically reacted in ELISA with 66% of sera from zoster patients and 35% of sera from control groups including sera containing antibodies to other herpes viruses, sera from healthy donors, and sera from patients with different forms of intestinal disorders. Consequently, we demonstrated possibility of application of our recombinant GE VZV as antigen for diagnostics and research use.https://www.epidemvac.ru/jour/article/view/131вирус varicella zosterorf68гликопротеин ерекомбинантный антигениммуноферментный анализvaricella zoster virus, vzvopen reading frame 68, orf 68glycoprotein grecombinant antigenenzyme immunoassay |
| spellingShingle | G. I. Alatortseva A. V. Sidorov L. N. Nesterenko L. N. Luhverchik I. I. Amiantova V. V. Docenko Phong Pham Huy A. V. Milovanova O. B. Vylivannaya A. C. Kazanova V. V. Lavrov V. V. Zverev Recombinant Analogue of Varicella Zoster Virus Glycoprotein E: Cloning, Expression and Studying of AntigEn Properties Эпидемиология и вакцинопрофилактика вирус varicella zoster orf68 гликопротеин е рекомбинантный антиген иммуноферментный анализ varicella zoster virus, vzv open reading frame 68, orf 68 glycoprotein g recombinant antigen enzyme immunoassay |
| title | Recombinant Analogue of Varicella Zoster Virus Glycoprotein E: Cloning, Expression and Studying of AntigEn Properties |
| title_full | Recombinant Analogue of Varicella Zoster Virus Glycoprotein E: Cloning, Expression and Studying of AntigEn Properties |
| title_fullStr | Recombinant Analogue of Varicella Zoster Virus Glycoprotein E: Cloning, Expression and Studying of AntigEn Properties |
| title_full_unstemmed | Recombinant Analogue of Varicella Zoster Virus Glycoprotein E: Cloning, Expression and Studying of AntigEn Properties |
| title_short | Recombinant Analogue of Varicella Zoster Virus Glycoprotein E: Cloning, Expression and Studying of AntigEn Properties |
| title_sort | recombinant analogue of varicella zoster virus glycoprotein e cloning expression and studying of antigen properties |
| topic | вирус varicella zoster orf68 гликопротеин е рекомбинантный антиген иммуноферментный анализ varicella zoster virus, vzv open reading frame 68, orf 68 glycoprotein g recombinant antigen enzyme immunoassay |
| url | https://www.epidemvac.ru/jour/article/view/131 |
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