Extraction of methamphetamine and pseudoephedrine by modified graphene oxide solid phase extraction method coupled to HPLC-UV in urine sample

Abstract Methamphetamine, pseudoephedrine, and related drugs are among the first drugs used for the stimulation of the central nervous system. In this study, two adsorbents based on graphene oxide (GO) were synthesized and used for the analysis of methamphetamine and pseudoephedrine. The prepared na...

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Main Authors: Nasim Nourani, Yousef Javadzadeh, Ali Shayanfar, Arezou Taghvimi, Ahad Bavili-Tabrizi, Siavoush Dastmalchi
Format: Article
Language:English
Published: BMC 2024-11-01
Series:BMC Chemistry
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Online Access:https://doi.org/10.1186/s13065-024-01331-y
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author Nasim Nourani
Yousef Javadzadeh
Ali Shayanfar
Arezou Taghvimi
Ahad Bavili-Tabrizi
Siavoush Dastmalchi
author_facet Nasim Nourani
Yousef Javadzadeh
Ali Shayanfar
Arezou Taghvimi
Ahad Bavili-Tabrizi
Siavoush Dastmalchi
author_sort Nasim Nourani
collection DOAJ
description Abstract Methamphetamine, pseudoephedrine, and related drugs are among the first drugs used for the stimulation of the central nervous system. In this study, two adsorbents based on graphene oxide (GO) were synthesized and used for the analysis of methamphetamine and pseudoephedrine. The prepared nano-adsorbents based on GO in this study were coated by polyaniline (PANI) and Fe3O4/C-nanodot/GO (magnetic adsorbent). The average size of nanoparticles (GO/PANI) was 18.43 nm. The specific surface area and pore size diameter of Fe3O4/C-nanodot/GO were 22.71 m2 g− 1 and 15.23 nm, respectively. Experimental variables affecting the extraction efficiency of the analytes such as pH of the sample solution, amount of adsorbent, extraction time, and type of eluents were investigated and optimized by response surface methodology. Under optimum conditions, GO/PANI and Fe3O4/C-nanodot/GO were considered appropriate solid phase extraction adsorbents for HPLC-based analyses of the studied drugs in human urine samples. However, GO/Fe3O4 nano adsorbent (Fe3O4/C-nanodot/GO) showed superior working condition than GO/PANI. The validated proposed analytical methods can be used for the quantitative determination of methamphetamine and pseudoephedrine in actual samples.
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spelling doaj-art-14cfa0430d9c4b26b39068271c13ac4c2024-11-10T12:05:07ZengBMCBMC Chemistry2661-801X2024-11-0118111310.1186/s13065-024-01331-yExtraction of methamphetamine and pseudoephedrine by modified graphene oxide solid phase extraction method coupled to HPLC-UV in urine sampleNasim Nourani0Yousef Javadzadeh1Ali Shayanfar2Arezou Taghvimi3Ahad Bavili-Tabrizi4Siavoush Dastmalchi5Student Research Committee, Tabriz University of Medical SciencesBiotechnology Research Center, Tabriz University of Medical SciencesPharmaceutical Analysis Research Center, Tabriz University of Medical SciencesBiotechnology Research Center, Tabriz University of Medical SciencesDepartment of Medicinal Chemistry, Faculty of Pharmacy, Tabriz University of Medical SciencesBiotechnology Research Center, Tabriz University of Medical SciencesAbstract Methamphetamine, pseudoephedrine, and related drugs are among the first drugs used for the stimulation of the central nervous system. In this study, two adsorbents based on graphene oxide (GO) were synthesized and used for the analysis of methamphetamine and pseudoephedrine. The prepared nano-adsorbents based on GO in this study were coated by polyaniline (PANI) and Fe3O4/C-nanodot/GO (magnetic adsorbent). The average size of nanoparticles (GO/PANI) was 18.43 nm. The specific surface area and pore size diameter of Fe3O4/C-nanodot/GO were 22.71 m2 g− 1 and 15.23 nm, respectively. Experimental variables affecting the extraction efficiency of the analytes such as pH of the sample solution, amount of adsorbent, extraction time, and type of eluents were investigated and optimized by response surface methodology. Under optimum conditions, GO/PANI and Fe3O4/C-nanodot/GO were considered appropriate solid phase extraction adsorbents for HPLC-based analyses of the studied drugs in human urine samples. However, GO/Fe3O4 nano adsorbent (Fe3O4/C-nanodot/GO) showed superior working condition than GO/PANI. The validated proposed analytical methods can be used for the quantitative determination of methamphetamine and pseudoephedrine in actual samples.https://doi.org/10.1186/s13065-024-01331-yMethamphetaminePseudoephedrineSolid phase extractionGraphene oxideUrine
spellingShingle Nasim Nourani
Yousef Javadzadeh
Ali Shayanfar
Arezou Taghvimi
Ahad Bavili-Tabrizi
Siavoush Dastmalchi
Extraction of methamphetamine and pseudoephedrine by modified graphene oxide solid phase extraction method coupled to HPLC-UV in urine sample
BMC Chemistry
Methamphetamine
Pseudoephedrine
Solid phase extraction
Graphene oxide
Urine
title Extraction of methamphetamine and pseudoephedrine by modified graphene oxide solid phase extraction method coupled to HPLC-UV in urine sample
title_full Extraction of methamphetamine and pseudoephedrine by modified graphene oxide solid phase extraction method coupled to HPLC-UV in urine sample
title_fullStr Extraction of methamphetamine and pseudoephedrine by modified graphene oxide solid phase extraction method coupled to HPLC-UV in urine sample
title_full_unstemmed Extraction of methamphetamine and pseudoephedrine by modified graphene oxide solid phase extraction method coupled to HPLC-UV in urine sample
title_short Extraction of methamphetamine and pseudoephedrine by modified graphene oxide solid phase extraction method coupled to HPLC-UV in urine sample
title_sort extraction of methamphetamine and pseudoephedrine by modified graphene oxide solid phase extraction method coupled to hplc uv in urine sample
topic Methamphetamine
Pseudoephedrine
Solid phase extraction
Graphene oxide
Urine
url https://doi.org/10.1186/s13065-024-01331-y
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