Distribution and Molecular Characterization of Antibiotic-Resistant Pseudomonas aeruginosa in Hospital Settings of Sulaymaniyah, Iraq
Pseudomonas aeruginosa is a significant pathogen in hospital settings, notorious for its role in hospital-acquired infections and its ability to develop resistance to multiple antibiotics. This study investigates the prevalence, distribution, and antibiotic resistance gene profiles of P. aeruginosa...
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| Format: | Article |
| Language: | English |
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Sciendo
2024-10-01
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| Series: | Polish Journal of Microbiology |
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| Online Access: | https://doi.org/10.33073/pjm-2024-037 |
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| author | Ali Seenaa Muhammed Soor Taib Ahmed Hama Ahmed Gashin Awat Mhdin Glena Aziz Othman Gulabakh Ali Faiq Sarkhel Mhamad |
| author_facet | Ali Seenaa Muhammed Soor Taib Ahmed Hama Ahmed Gashin Awat Mhdin Glena Aziz Othman Gulabakh Ali Faiq Sarkhel Mhamad |
| author_sort | Ali Seenaa Muhammed |
| collection | DOAJ |
| description | Pseudomonas aeruginosa is a significant pathogen in hospital settings, notorious for its role in hospital-acquired infections and its ability to develop resistance to multiple antibiotics. This study investigates the prevalence, distribution, and antibiotic resistance gene profiles of P. aeruginosa in seven hospitals in Sulaymaniyah City. A total of 300 samples were collected from various hospital surfaces including mops, sinks, medical equipment, beds, desks, and floors. Using bacteriological, biochemical, and molecular methods, 66 isolates were confirmed as Pseudomonas species, with 26 identified as P. aeruginosa. Antibiotic susceptibility testing revealed resistance rates of 23.3% to streptomycin, 13.6% to tobramycin, 22.7% to moxifloxacin, 21.2% to levofloxacin, and 22.7% to norfloxacin. Furthermore, the antibiotic resistance gene detection showed the presence of the blaCTX-M, blaSHV, qnrB, and blaACC-1 genes among the isolates. The study highlights a 22% contamination rate of hospital surfaces with Pseudomonas species, emphasizing the urgent need for enhanced infection control measures and targeted antimicrobial stewardship to manage and reduce the spread of multidrug-resistant P. aeruginosa. |
| format | Article |
| id | doaj-art-14cbe927ad8b4a32b43520d8d6559af8 |
| institution | Kabale University |
| issn | 2544-4646 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Polish Journal of Microbiology |
| spelling | doaj-art-14cbe927ad8b4a32b43520d8d6559af82024-12-16T08:59:10ZengSciendoPolish Journal of Microbiology2544-46462024-10-0173446747310.33073/pjm-2024-037Distribution and Molecular Characterization of Antibiotic-Resistant Pseudomonas aeruginosa in Hospital Settings of Sulaymaniyah, IraqAli Seenaa Muhammed0Soor Taib Ahmed Hama1Ahmed Gashin Awat2Mhdin Glena Aziz3Othman Gulabakh Ali4Faiq Sarkhel Mhamad5Medical Laboratory Department, Technical College of Health and Medical Technology, Sulaimani Polytechnic University, Sulaymaniyah, IraqMedical Laboratory Department, Technical College of Health and Medical Technology, Sulaimani Polytechnic University, Sulaymaniyah, IraqMedical Laboratory Department, Technical College of Health and Medical Technology, Sulaimani Polytechnic University, Sulaymaniyah, IraqMedical Laboratory Department, Technical College of Health and Medical Technology, Sulaimani Polytechnic University, Sulaymaniyah, IraqMedical Laboratory Department, Technical College of Health and Medical Technology, Sulaimani Polytechnic University, Sulaymaniyah, IraqMedical Laboratory Department, Technical College of Health and Medical Technology, Sulaimani Polytechnic University, Sulaymaniyah, IraqPseudomonas aeruginosa is a significant pathogen in hospital settings, notorious for its role in hospital-acquired infections and its ability to develop resistance to multiple antibiotics. This study investigates the prevalence, distribution, and antibiotic resistance gene profiles of P. aeruginosa in seven hospitals in Sulaymaniyah City. A total of 300 samples were collected from various hospital surfaces including mops, sinks, medical equipment, beds, desks, and floors. Using bacteriological, biochemical, and molecular methods, 66 isolates were confirmed as Pseudomonas species, with 26 identified as P. aeruginosa. Antibiotic susceptibility testing revealed resistance rates of 23.3% to streptomycin, 13.6% to tobramycin, 22.7% to moxifloxacin, 21.2% to levofloxacin, and 22.7% to norfloxacin. Furthermore, the antibiotic resistance gene detection showed the presence of the blaCTX-M, blaSHV, qnrB, and blaACC-1 genes among the isolates. The study highlights a 22% contamination rate of hospital surfaces with Pseudomonas species, emphasizing the urgent need for enhanced infection control measures and targeted antimicrobial stewardship to manage and reduce the spread of multidrug-resistant P. aeruginosa.https://doi.org/10.33073/pjm-2024-037pseudomonas aeruginosaantibiotic resistanceresistance geneshospital surfacesmolecular characterization |
| spellingShingle | Ali Seenaa Muhammed Soor Taib Ahmed Hama Ahmed Gashin Awat Mhdin Glena Aziz Othman Gulabakh Ali Faiq Sarkhel Mhamad Distribution and Molecular Characterization of Antibiotic-Resistant Pseudomonas aeruginosa in Hospital Settings of Sulaymaniyah, Iraq Polish Journal of Microbiology pseudomonas aeruginosa antibiotic resistance resistance genes hospital surfaces molecular characterization |
| title | Distribution and Molecular Characterization of Antibiotic-Resistant Pseudomonas aeruginosa in Hospital Settings of Sulaymaniyah, Iraq |
| title_full | Distribution and Molecular Characterization of Antibiotic-Resistant Pseudomonas aeruginosa in Hospital Settings of Sulaymaniyah, Iraq |
| title_fullStr | Distribution and Molecular Characterization of Antibiotic-Resistant Pseudomonas aeruginosa in Hospital Settings of Sulaymaniyah, Iraq |
| title_full_unstemmed | Distribution and Molecular Characterization of Antibiotic-Resistant Pseudomonas aeruginosa in Hospital Settings of Sulaymaniyah, Iraq |
| title_short | Distribution and Molecular Characterization of Antibiotic-Resistant Pseudomonas aeruginosa in Hospital Settings of Sulaymaniyah, Iraq |
| title_sort | distribution and molecular characterization of antibiotic resistant pseudomonas aeruginosa in hospital settings of sulaymaniyah iraq |
| topic | pseudomonas aeruginosa antibiotic resistance resistance genes hospital surfaces molecular characterization |
| url | https://doi.org/10.33073/pjm-2024-037 |
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