Model System for Live Imaging of Neuronal Responses to Injury and Repair
Although it has been well established that induction of growth-associated protein-43 (GAP-43) during development coincides with axonal outgrowth and early synapse formation, the existence of neuronal plasticity and neurite outgrowth in the adult central nervous system after injuries is more controve...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2011-11-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2011.00013 |
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| _version_ | 1841564628853719040 |
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| author | Mathieu Gravel Yuan-Cheng Weng Jasna Kriz |
| author_facet | Mathieu Gravel Yuan-Cheng Weng Jasna Kriz |
| author_sort | Mathieu Gravel |
| collection | DOAJ |
| description | Although it has been well established that induction of growth-associated protein-43 (GAP-43) during development coincides with axonal outgrowth and early synapse formation, the existence of neuronal plasticity and neurite outgrowth in the adult central nervous system after injuries is more controversial. To visualize the processes of neuronal injury and repair in living animals, we generated reporter mice for bioluminescence and fluorescence imaging bearing the luc (luciferase) and gfp (green fluorescent protein) reporter genes under the control of the murine GAP-43 promoter. Reporter functionality was first observed during the development of transgenic embryos. Using in vivo bioluminescence and fluorescence imaging, we visualized induction of the GAP-43 signals from live embryos starting at E10.5, as well as neuronal responses to brain and peripheral nerve injuries (the signals peaked at 14 days postinjury). Moreover, three-dimensional analysis of the GAP-43 bioluminescent signal confirmed that it originated from brain structures affected by ischemic injury. The analysis of fluorescence signal at cellular level revealed colocalization between endogenous protein and the GAP-43-driven gfp transgene. Taken together, our results suggest that the GAP-43-luc/gfp reporter mouse represents a valid model system for real-time analysis of neurite outgrowth and the capacity of the adult nervous system to regenerate after injuries. |
| format | Article |
| id | doaj-art-149768b36b74417bad1477616ee6fb11 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2011-11-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-149768b36b74417bad1477616ee6fb112025-01-02T22:37:09ZengSAGE PublishingMolecular Imaging1536-01212011-11-011010.2310/7290.2011.0001310.2310_7290.2011.00013Model System for Live Imaging of Neuronal Responses to Injury and RepairMathieu GravelYuan-Cheng WengJasna KrizAlthough it has been well established that induction of growth-associated protein-43 (GAP-43) during development coincides with axonal outgrowth and early synapse formation, the existence of neuronal plasticity and neurite outgrowth in the adult central nervous system after injuries is more controversial. To visualize the processes of neuronal injury and repair in living animals, we generated reporter mice for bioluminescence and fluorescence imaging bearing the luc (luciferase) and gfp (green fluorescent protein) reporter genes under the control of the murine GAP-43 promoter. Reporter functionality was first observed during the development of transgenic embryos. Using in vivo bioluminescence and fluorescence imaging, we visualized induction of the GAP-43 signals from live embryos starting at E10.5, as well as neuronal responses to brain and peripheral nerve injuries (the signals peaked at 14 days postinjury). Moreover, three-dimensional analysis of the GAP-43 bioluminescent signal confirmed that it originated from brain structures affected by ischemic injury. The analysis of fluorescence signal at cellular level revealed colocalization between endogenous protein and the GAP-43-driven gfp transgene. Taken together, our results suggest that the GAP-43-luc/gfp reporter mouse represents a valid model system for real-time analysis of neurite outgrowth and the capacity of the adult nervous system to regenerate after injuries.https://doi.org/10.2310/7290.2011.00013 |
| spellingShingle | Mathieu Gravel Yuan-Cheng Weng Jasna Kriz Model System for Live Imaging of Neuronal Responses to Injury and Repair Molecular Imaging |
| title | Model System for Live Imaging of Neuronal Responses to Injury and Repair |
| title_full | Model System for Live Imaging of Neuronal Responses to Injury and Repair |
| title_fullStr | Model System for Live Imaging of Neuronal Responses to Injury and Repair |
| title_full_unstemmed | Model System for Live Imaging of Neuronal Responses to Injury and Repair |
| title_short | Model System for Live Imaging of Neuronal Responses to Injury and Repair |
| title_sort | model system for live imaging of neuronal responses to injury and repair |
| url | https://doi.org/10.2310/7290.2011.00013 |
| work_keys_str_mv | AT mathieugravel modelsystemforliveimagingofneuronalresponsestoinjuryandrepair AT yuanchengweng modelsystemforliveimagingofneuronalresponsestoinjuryandrepair AT jasnakriz modelsystemforliveimagingofneuronalresponsestoinjuryandrepair |