Impact of altitudinal variation on secondary metabolites, antioxidant, anti-inflammatory and anticancer potential of Adiantum venustum D. Don
Phytoconstituents are being emphasized as chemotherapeutic agents, as well as probable anticancer agents. The purpose of the present research was to assess the antioxidant, anti-inflammatory, and anticancer properties of crude ethanolic extracts and bioactive fractions of Adiantum venustum D. Don co...
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Elsevier
2024-12-01
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| Series: | Journal of Agriculture and Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266615432400334X |
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| author | Neetika Kimta Sunil Puri Amita Kumari Byung Hyune Choi Eugenie Nepovimova Suliman Alomar Kamil Kuca |
| author_facet | Neetika Kimta Sunil Puri Amita Kumari Byung Hyune Choi Eugenie Nepovimova Suliman Alomar Kamil Kuca |
| author_sort | Neetika Kimta |
| collection | DOAJ |
| description | Phytoconstituents are being emphasized as chemotherapeutic agents, as well as probable anticancer agents. The purpose of the present research was to assess the antioxidant, anti-inflammatory, and anticancer properties of crude ethanolic extracts and bioactive fractions of Adiantum venustum D. Don collected from three sites in Himachal Pradesh, India. The bioactive phytoconstituents were examined qualitatively and quantitatively in crude ethanolic extracts and fractions of all sites, and biological activities were analyzed. The potential presence of terpenoids, saponins, tannins, phenols, and flavonoids was examined, and it came to light that BF-II [n-butanol fraction of Site-II (2000 m altitude)] had a significantly higher phytochemical concentration. Additionally, the crude ethanolic extracts and fractions were examined for antioxidant and anti-inflammatory properties, and BF-II was found to have potential antioxidant and anti-inflammatory activity, as verified by the IC50 values (lowest half inhibitory concentration) in the DPPH (57.86 ± 0.72 μg/mL), reducing power assay (45.20 ± 0.72 μg/mL), FRAP (73.12 ± 0.23 μM Fe equivalent), BSA (56.76 ± 0.25 μg/mL), and EAA (EAA59.84 ± 0.13 μg/mL). Following that, the anticancer potential was investigated further using the MTT assay on the HeLa cell line, MTT assay revealed that BF-II possesses significantly higher anticancer potential. Furthermore, the dominant crude ethanolic extract and fraction were further investigated using gas chromatography-mass spectroscopy, and high-performance thin layer chromatography techniques from the selected plant. Overall, the study suggests that the n-butanol fraction of A. venustum possesses significantly higher therapeutic value but is still not explored to the extent of its potential. |
| format | Article |
| id | doaj-art-147998af6fa34bf1b2178fc0bf5d981c |
| institution | Kabale University |
| issn | 2666-1543 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Agriculture and Food Research |
| spelling | doaj-art-147998af6fa34bf1b2178fc0bf5d981c2024-12-16T05:37:15ZengElsevierJournal of Agriculture and Food Research2666-15432024-12-0118101297Impact of altitudinal variation on secondary metabolites, antioxidant, anti-inflammatory and anticancer potential of Adiantum venustum D. DonNeetika Kimta0Sunil Puri1Amita Kumari2Byung Hyune Choi3Eugenie Nepovimova4Suliman Alomar5Kamil Kuca6School of Biological and Environmental Sciences, Shoolini University of Biotechnology and Management Sciences, Solan, 173229, IndiaSchool of Biological and Environmental Sciences, Shoolini University of Biotechnology and Management Sciences, Solan, 173229, India; Corresponding author.Patanjali Herbal Research Department, Patanjali Research Institute, Haridwar, Uttarakhand, 249405, IndiaDepartment of Biomedical Sciences, Inha University College of Medicine, 100 Inha-ro, Incheon, 22212, South KoreaDepartment of Chemistry, Faculty of Science, University of Hradec Kralove, 50005, HradecKralove, Czech RepublicZoology Department, College of Science, King Saud University, Saudi ArabiaDepartment of Chemistry, Faculty of Science, University of Hradec Kralove, 50005, HradecKralove, Czech Republic; Biomedical Research Center, University Hospital Hradec Kralove, 50005, Hradec Kralove, Czech Republic; Corresponding author. Department of Chemistry, Faculty of Science, University of Hradec Kralove, 50005, Hradec Kralove, Czech Republic.Phytoconstituents are being emphasized as chemotherapeutic agents, as well as probable anticancer agents. The purpose of the present research was to assess the antioxidant, anti-inflammatory, and anticancer properties of crude ethanolic extracts and bioactive fractions of Adiantum venustum D. Don collected from three sites in Himachal Pradesh, India. The bioactive phytoconstituents were examined qualitatively and quantitatively in crude ethanolic extracts and fractions of all sites, and biological activities were analyzed. The potential presence of terpenoids, saponins, tannins, phenols, and flavonoids was examined, and it came to light that BF-II [n-butanol fraction of Site-II (2000 m altitude)] had a significantly higher phytochemical concentration. Additionally, the crude ethanolic extracts and fractions were examined for antioxidant and anti-inflammatory properties, and BF-II was found to have potential antioxidant and anti-inflammatory activity, as verified by the IC50 values (lowest half inhibitory concentration) in the DPPH (57.86 ± 0.72 μg/mL), reducing power assay (45.20 ± 0.72 μg/mL), FRAP (73.12 ± 0.23 μM Fe equivalent), BSA (56.76 ± 0.25 μg/mL), and EAA (EAA59.84 ± 0.13 μg/mL). Following that, the anticancer potential was investigated further using the MTT assay on the HeLa cell line, MTT assay revealed that BF-II possesses significantly higher anticancer potential. Furthermore, the dominant crude ethanolic extract and fraction were further investigated using gas chromatography-mass spectroscopy, and high-performance thin layer chromatography techniques from the selected plant. Overall, the study suggests that the n-butanol fraction of A. venustum possesses significantly higher therapeutic value but is still not explored to the extent of its potential.http://www.sciencedirect.com/science/article/pii/S266615432400334XBioactive phytoconstituentsAntioxidantsAnti-inflammatory activityAnticancer potentialHeLa cells |
| spellingShingle | Neetika Kimta Sunil Puri Amita Kumari Byung Hyune Choi Eugenie Nepovimova Suliman Alomar Kamil Kuca Impact of altitudinal variation on secondary metabolites, antioxidant, anti-inflammatory and anticancer potential of Adiantum venustum D. Don Journal of Agriculture and Food Research Bioactive phytoconstituents Antioxidants Anti-inflammatory activity Anticancer potential HeLa cells |
| title | Impact of altitudinal variation on secondary metabolites, antioxidant, anti-inflammatory and anticancer potential of Adiantum venustum D. Don |
| title_full | Impact of altitudinal variation on secondary metabolites, antioxidant, anti-inflammatory and anticancer potential of Adiantum venustum D. Don |
| title_fullStr | Impact of altitudinal variation on secondary metabolites, antioxidant, anti-inflammatory and anticancer potential of Adiantum venustum D. Don |
| title_full_unstemmed | Impact of altitudinal variation on secondary metabolites, antioxidant, anti-inflammatory and anticancer potential of Adiantum venustum D. Don |
| title_short | Impact of altitudinal variation on secondary metabolites, antioxidant, anti-inflammatory and anticancer potential of Adiantum venustum D. Don |
| title_sort | impact of altitudinal variation on secondary metabolites antioxidant anti inflammatory and anticancer potential of adiantum venustum d don |
| topic | Bioactive phytoconstituents Antioxidants Anti-inflammatory activity Anticancer potential HeLa cells |
| url | http://www.sciencedirect.com/science/article/pii/S266615432400334X |
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