生长激素促进体外培养的Bel-7402肝癌细胞增殖

【目的】 了解重组人生长激素(rhGH)对体外培养的Bel-7402肝癌细胞增殖的影响65377;【方法】 应用放射性配体法了解GHR在Bel-7402肝癌细胞株的表达情况;采用肿瘤细胞计数65380;噻唑蓝比色法和集落形成实验了解Bel-7402肝癌细胞株在不同浓度rhGH(065380;165380;1065380;10065380;1 00065380;10 000 ng/mL)作用下的药物敏感性,计算细胞生长率,集落形成率;并用3H-TdR渗入法了解肿瘤细胞DNA代谢情况65377;【结果】 放射配体法发现Bel-7402肝癌细胞表达GHR, rhGH对体外培养的7402肝癌细胞的生长...

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Bibliographic Details
Format: Article
Language:zho
Published: Editorial Office of Journal of Sun Yat-sen University 2008-01-01
Series:Zhongshan Daxue xuebao. Yixue kexue ban
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Online Access:http://xuebaoyx.sysu.edu.cn/zh/article/43580539/
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