A Global Collaborative Comparison of SARS-CoV-2 Antigenicity Across 15 Laboratories
Setting up a global SARS-CoV-2 surveillance system requires an understanding of how virus isolation and propagation practices, use of animal or human sera, and different neutralisation assay platforms influence assessment of SARS-CoV-2 antigenicity. In this study, with the contribution of 15 indepen...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/16/12/1936 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846102325541732352 |
|---|---|
| author | Polina Brangel Sina Tureli Barbara Mühlemann Nicole Liechti Daniel Zysset Olivier Engler Isabel Hunger-Glaser Ioana Ghiga Giada Mattiuzzo Isabella Eckerle Meriem Bekliz Annika Rössler Melanie M. Schmitt Ludwig Knabl Janine Kimpel Luis Fernando Lopez Tort Mia Ferreira de Araujo Any Caroline Alves de Oliveira Braulia Costa Caetano Marilda Mendonça Siqueira Matthias Budt Jean-Marc Gensch Thorsten Wolff Tarteel Hassan Francis Amirtharaj Selvaraj Tandile Hermanus Prudence Kgagudi Carol Crowther Simone I. Richardson Jinal N. Bhiman Penny L. Moore Samuel M. S. Cheng John K. C. Li Leo L. M. Poon Malik Peiris Victor M. Corman Christian Drosten Lilin Lai Taweewun Hunsawong Kamonthip Rungrojcharoenkit Jindarat Lohachanakul Alex Sigal Khadija Khan Volker Thiel G. Tuba Barut Nadine Ebert Anna Z. Mykytyn Irene Owusu Donkor James Odame Aboagye Prince Adom Nartey Maria D. Van Kerkhove Jane Cunningham Bart L. Haagmans Mehul S. Suthar Derek Smith Lorenzo Subissi |
| author_facet | Polina Brangel Sina Tureli Barbara Mühlemann Nicole Liechti Daniel Zysset Olivier Engler Isabel Hunger-Glaser Ioana Ghiga Giada Mattiuzzo Isabella Eckerle Meriem Bekliz Annika Rössler Melanie M. Schmitt Ludwig Knabl Janine Kimpel Luis Fernando Lopez Tort Mia Ferreira de Araujo Any Caroline Alves de Oliveira Braulia Costa Caetano Marilda Mendonça Siqueira Matthias Budt Jean-Marc Gensch Thorsten Wolff Tarteel Hassan Francis Amirtharaj Selvaraj Tandile Hermanus Prudence Kgagudi Carol Crowther Simone I. Richardson Jinal N. Bhiman Penny L. Moore Samuel M. S. Cheng John K. C. Li Leo L. M. Poon Malik Peiris Victor M. Corman Christian Drosten Lilin Lai Taweewun Hunsawong Kamonthip Rungrojcharoenkit Jindarat Lohachanakul Alex Sigal Khadija Khan Volker Thiel G. Tuba Barut Nadine Ebert Anna Z. Mykytyn Irene Owusu Donkor James Odame Aboagye Prince Adom Nartey Maria D. Van Kerkhove Jane Cunningham Bart L. Haagmans Mehul S. Suthar Derek Smith Lorenzo Subissi |
| author_sort | Polina Brangel |
| collection | DOAJ |
| description | Setting up a global SARS-CoV-2 surveillance system requires an understanding of how virus isolation and propagation practices, use of animal or human sera, and different neutralisation assay platforms influence assessment of SARS-CoV-2 antigenicity. In this study, with the contribution of 15 independent laboratories across all WHO regions, we carried out a controlled analysis of neutralisation assay platforms using the first WHO International Standard for antibodies to SARS-CoV-2 variants of concern (source: NIBSC). Live virus isolates (source: WHO BioHub or individual labs) or spike plasmids (individual labs) for pseudovirus production were used to perform neutralisation assays using the same serum panels. When comparing fold drops, excellent data consistency was observed across the labs using common reagents, including between pseudovirus and live virus neutralisation assays (RMSD of data from mean fold drop was 0.59). Utilising a Bayesian model, geometric mean titres and assay titre magnitudes (offsets) can describe the data efficiently. Titre magnitudes were seen to vary largely even for labs within the same assay group. We have observed that overall, live Microneutralisation assays tend to have the lowest titres, whereas Pseudovirus Neutralisation have the highest (with a mean difference of 3.2 log2 units between the two). These findings are relevant for laboratory networks, such as the WHO Coronavirus Laboratory Network (CoViNet), that seek to support a global surveillance system for evolution and antigenic characterisation of variants to support monitoring of population immunity and vaccine composition policy. |
| format | Article |
| id | doaj-art-14699f78c404432c848db5d5389e47d0 |
| institution | Kabale University |
| issn | 1999-4915 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-14699f78c404432c848db5d5389e47d02024-12-27T14:59:18ZengMDPI AGViruses1999-49152024-12-011612193610.3390/v16121936A Global Collaborative Comparison of SARS-CoV-2 Antigenicity Across 15 LaboratoriesPolina Brangel0Sina Tureli1Barbara Mühlemann2Nicole Liechti3Daniel Zysset4Olivier Engler5Isabel Hunger-Glaser6Ioana Ghiga7Giada Mattiuzzo8Isabella Eckerle9Meriem Bekliz10Annika Rössler11Melanie M. Schmitt12Ludwig Knabl13Janine Kimpel14Luis Fernando Lopez Tort15Mia Ferreira de Araujo16Any Caroline Alves de Oliveira17Braulia Costa Caetano18Marilda Mendonça Siqueira19Matthias Budt20Jean-Marc Gensch21Thorsten Wolff22Tarteel Hassan23Francis Amirtharaj Selvaraj24Tandile Hermanus25Prudence Kgagudi26Carol Crowther27Simone I. Richardson28Jinal N. Bhiman29Penny L. Moore30Samuel M. S. Cheng31John K. C. Li32Leo L. M. Poon33Malik Peiris34Victor M. Corman35Christian Drosten36Lilin Lai37Taweewun Hunsawong38Kamonthip Rungrojcharoenkit39Jindarat Lohachanakul40Alex Sigal41Khadija Khan42Volker Thiel43G. Tuba Barut44Nadine Ebert45Anna Z. Mykytyn46Irene Owusu Donkor47James Odame Aboagye48Prince Adom Nartey49Maria D. Van Kerkhove50Jane Cunningham51Bart L. Haagmans52Mehul S. Suthar53Derek Smith54Lorenzo Subissi55World Health Organization, 1202 Geneva, SwitzerlandCentre for Pathogen Evolution, University of Cambridge, Cambridge CB3 0FD, UKCharité–Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Institute of Virology, Charitéplatz 1, 10117 Berlin, GermanyWHO BioHub Facility, Spiez Laboratory, 3700 Spiez, SwitzerlandWHO BioHub Facility, Spiez Laboratory, 3700 Spiez, SwitzerlandWHO BioHub Facility, Spiez Laboratory, 3700 Spiez, SwitzerlandWHO BioHub Facility, Spiez Laboratory, 3700 Spiez, SwitzerlandWorld Health Organization, 1202 Geneva, SwitzerlandMedicines and Healthcare Products Regulatory Agency, London SW1W 9SZ, UKDepartment of Medicine, University of Geneva, 1205 Geneva, SwitzerlandDepartment of Medicine, University of Geneva, 1205 Geneva, SwitzerlandInstitute of Virology, Department of Hygiene, Microbiology and Virology, Medical University of Innsbruck, 6020 Innsbruck, AustriaInstitute of Virology, Department of Hygiene, Microbiology and Virology, Medical University of Innsbruck, 6020 Innsbruck, AustriaTyrolpath Obrist Brunhuber GmbH, 6511 Zams, AustriaInstitute of Virology, Department of Hygiene, Microbiology and Virology, Medical University of Innsbruck, 6020 Innsbruck, AustriaLaboratory of Respiratory, Exanthematous and Enteric Viruses and Viral Emergencies (LVRE), Oswaldo Cruz Institute (IOC-Fiocruz), Rio de Janeiro 21.040-900, BrazilLaboratory of Respiratory, Exanthematous and Enteric Viruses and Viral Emergencies (LVRE), Oswaldo Cruz Institute (IOC-Fiocruz), Rio de Janeiro 21.040-900, BrazilLaboratory of Respiratory, Exanthematous and Enteric Viruses and Viral Emergencies (LVRE), Oswaldo Cruz Institute (IOC-Fiocruz), Rio de Janeiro 21.040-900, BrazilLaboratory of Respiratory, Exanthematous and Enteric Viruses and Viral Emergencies (LVRE), Oswaldo Cruz Institute (IOC-Fiocruz), Rio de Janeiro 21.040-900, BrazilLaboratory of Respiratory, Exanthematous and Enteric Viruses and Viral Emergencies (LVRE), Oswaldo Cruz Institute (IOC-Fiocruz), Rio de Janeiro 21.040-900, BrazilUnit 17 “Influenza and Other Respiratory Viruses”, Robert Koch Institut, 13353 Berlin, GermanyUnit 17 “Influenza and Other Respiratory Viruses”, Robert Koch Institut, 13353 Berlin, GermanyUnit 17 “Influenza and Other Respiratory Viruses”, Robert Koch Institut, 13353 Berlin, GermanyReference Laboratory for Infectious Diseases, Purelab, Sheikh Khalifa Medical City, Abu Dhabi 51900, United Arab EmiratesReference Laboratory for Infectious Diseases, Purelab, Sheikh Khalifa Medical City, Abu Dhabi 51900, United Arab EmiratesSAMRC Antibody Immunity Research Unit, School of Pathology, University of the Witwatersrand, Johannesburg 2001, South AfricaSAMRC Antibody Immunity Research Unit, School of Pathology, University of the Witwatersrand, Johannesburg 2001, South AfricaSAMRC Antibody Immunity Research Unit, School of Pathology, University of the Witwatersrand, Johannesburg 2001, South AfricaSAMRC Antibody Immunity Research Unit, School of Pathology, University of the Witwatersrand, Johannesburg 2001, South AfricaSAMRC Antibody Immunity Research Unit, School of Pathology, University of the Witwatersrand, Johannesburg 2001, South AfricaSAMRC Antibody Immunity Research Unit, School of Pathology, University of the Witwatersrand, Johannesburg 2001, South AfricaSchool of Public Health, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, ChinaSchool of Public Health, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, ChinaSchool of Public Health, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, ChinaSchool of Public Health, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, ChinaCharité–Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Institute of Virology, Charitéplatz 1, 10117 Berlin, GermanyCharité–Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Institute of Virology, Charitéplatz 1, 10117 Berlin, GermanyDepartment of Pediatrics, Center for Childhood Infections and Vaccines, Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322, USAWalter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences, Bangkok 10500, ThailandWalter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences, Bangkok 10500, ThailandWalter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences, Bangkok 10500, ThailandThe Lautenberg Center for Immunology and Cancer Research, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 91904, IsraelAfrica Health Research Institute, Durban 4013, South AfricaMultidisciplinary Center for Infectious Diseases, University of Bern, 3012 Bern, SwitzerlandMultidisciplinary Center for Infectious Diseases, University of Bern, 3012 Bern, SwitzerlandMultidisciplinary Center for Infectious Diseases, University of Bern, 3012 Bern, SwitzerlandErasmus Medical Centre, 3015 Rotterdam, The NetherlandsMedical and Scientific Research Centre, University of Ghana Medical Centre, Accra P.O. Box LG 25, GhanaMedical and Scientific Research Centre, University of Ghana Medical Centre, Accra P.O. Box LG 25, GhanaNoguchi Memorial Institute for Medical Research, University of Ghana, Legon P.O. Box LG 581, GhanaWorld Health Organization, 1202 Geneva, SwitzerlandWorld Health Organization, 1202 Geneva, SwitzerlandErasmus Medical Centre, 3015 Rotterdam, The NetherlandsDepartment of Pediatrics, Center for Childhood Infections and Vaccines, Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322, USACentre for Pathogen Evolution, University of Cambridge, Cambridge CB3 0FD, UKWorld Health Organization, 1202 Geneva, SwitzerlandSetting up a global SARS-CoV-2 surveillance system requires an understanding of how virus isolation and propagation practices, use of animal or human sera, and different neutralisation assay platforms influence assessment of SARS-CoV-2 antigenicity. In this study, with the contribution of 15 independent laboratories across all WHO regions, we carried out a controlled analysis of neutralisation assay platforms using the first WHO International Standard for antibodies to SARS-CoV-2 variants of concern (source: NIBSC). Live virus isolates (source: WHO BioHub or individual labs) or spike plasmids (individual labs) for pseudovirus production were used to perform neutralisation assays using the same serum panels. When comparing fold drops, excellent data consistency was observed across the labs using common reagents, including between pseudovirus and live virus neutralisation assays (RMSD of data from mean fold drop was 0.59). Utilising a Bayesian model, geometric mean titres and assay titre magnitudes (offsets) can describe the data efficiently. Titre magnitudes were seen to vary largely even for labs within the same assay group. We have observed that overall, live Microneutralisation assays tend to have the lowest titres, whereas Pseudovirus Neutralisation have the highest (with a mean difference of 3.2 log2 units between the two). These findings are relevant for laboratory networks, such as the WHO Coronavirus Laboratory Network (CoViNet), that seek to support a global surveillance system for evolution and antigenic characterisation of variants to support monitoring of population immunity and vaccine composition policy.https://www.mdpi.com/1999-4915/16/12/1936neutralisationantigenicitySARS-CoV-2COVID-19Bayesian modelglobal surveillance |
| spellingShingle | Polina Brangel Sina Tureli Barbara Mühlemann Nicole Liechti Daniel Zysset Olivier Engler Isabel Hunger-Glaser Ioana Ghiga Giada Mattiuzzo Isabella Eckerle Meriem Bekliz Annika Rössler Melanie M. Schmitt Ludwig Knabl Janine Kimpel Luis Fernando Lopez Tort Mia Ferreira de Araujo Any Caroline Alves de Oliveira Braulia Costa Caetano Marilda Mendonça Siqueira Matthias Budt Jean-Marc Gensch Thorsten Wolff Tarteel Hassan Francis Amirtharaj Selvaraj Tandile Hermanus Prudence Kgagudi Carol Crowther Simone I. Richardson Jinal N. Bhiman Penny L. Moore Samuel M. S. Cheng John K. C. Li Leo L. M. Poon Malik Peiris Victor M. Corman Christian Drosten Lilin Lai Taweewun Hunsawong Kamonthip Rungrojcharoenkit Jindarat Lohachanakul Alex Sigal Khadija Khan Volker Thiel G. Tuba Barut Nadine Ebert Anna Z. Mykytyn Irene Owusu Donkor James Odame Aboagye Prince Adom Nartey Maria D. Van Kerkhove Jane Cunningham Bart L. Haagmans Mehul S. Suthar Derek Smith Lorenzo Subissi A Global Collaborative Comparison of SARS-CoV-2 Antigenicity Across 15 Laboratories Viruses neutralisation antigenicity SARS-CoV-2 COVID-19 Bayesian model global surveillance |
| title | A Global Collaborative Comparison of SARS-CoV-2 Antigenicity Across 15 Laboratories |
| title_full | A Global Collaborative Comparison of SARS-CoV-2 Antigenicity Across 15 Laboratories |
| title_fullStr | A Global Collaborative Comparison of SARS-CoV-2 Antigenicity Across 15 Laboratories |
| title_full_unstemmed | A Global Collaborative Comparison of SARS-CoV-2 Antigenicity Across 15 Laboratories |
| title_short | A Global Collaborative Comparison of SARS-CoV-2 Antigenicity Across 15 Laboratories |
| title_sort | global collaborative comparison of sars cov 2 antigenicity across 15 laboratories |
| topic | neutralisation antigenicity SARS-CoV-2 COVID-19 Bayesian model global surveillance |
| url | https://www.mdpi.com/1999-4915/16/12/1936 |
| work_keys_str_mv | AT polinabrangel aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT sinatureli aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT barbaramuhlemann aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT nicoleliechti aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT danielzysset aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT olivierengler aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT isabelhungerglaser aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT ioanaghiga aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT giadamattiuzzo aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT isabellaeckerle aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT meriembekliz aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT annikarossler aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT melaniemschmitt aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT ludwigknabl aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT janinekimpel aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT luisfernandolopeztort aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT miaferreiradearaujo aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT anycarolinealvesdeoliveira aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT brauliacostacaetano aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT marildamendoncasiqueira aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT matthiasbudt aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT jeanmarcgensch aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT thorstenwolff aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT tarteelhassan aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT francisamirtharajselvaraj aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT tandilehermanus aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT prudencekgagudi aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT carolcrowther aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT simoneirichardson aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT jinalnbhiman aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT pennylmoore aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT samuelmscheng aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT johnkcli aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT leolmpoon aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT malikpeiris aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT victormcorman aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT christiandrosten aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT lilinlai aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT taweewunhunsawong aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT kamonthiprungrojcharoenkit aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT jindaratlohachanakul aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT alexsigal aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT khadijakhan aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT volkerthiel aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT gtubabarut aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT nadineebert aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT annazmykytyn aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT ireneowusudonkor aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT jamesodameaboagye aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT princeadomnartey aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT mariadvankerkhove aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT janecunningham aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT bartlhaagmans aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT mehulssuthar aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT dereksmith aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT lorenzosubissi aglobalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT polinabrangel globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT sinatureli globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT barbaramuhlemann globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT nicoleliechti globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT danielzysset globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT olivierengler globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT isabelhungerglaser globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT ioanaghiga globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT giadamattiuzzo globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT isabellaeckerle globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT meriembekliz globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT annikarossler globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT melaniemschmitt globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT ludwigknabl globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT janinekimpel globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT luisfernandolopeztort globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT miaferreiradearaujo globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT anycarolinealvesdeoliveira globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT brauliacostacaetano globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT marildamendoncasiqueira globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT matthiasbudt globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT jeanmarcgensch globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT thorstenwolff globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT tarteelhassan globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT francisamirtharajselvaraj globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT tandilehermanus globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT prudencekgagudi globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT carolcrowther globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT simoneirichardson globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT jinalnbhiman globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT pennylmoore globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT samuelmscheng globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT johnkcli globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT leolmpoon globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT malikpeiris globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT victormcorman globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT christiandrosten globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT lilinlai globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT taweewunhunsawong globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT kamonthiprungrojcharoenkit globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT jindaratlohachanakul globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT alexsigal globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT khadijakhan globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT volkerthiel globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT gtubabarut globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT nadineebert globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT annazmykytyn globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT ireneowusudonkor globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT jamesodameaboagye globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT princeadomnartey globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT mariadvankerkhove globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT janecunningham globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT bartlhaagmans globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT mehulssuthar globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT dereksmith globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories AT lorenzosubissi globalcollaborativecomparisonofsarscov2antigenicityacross15laboratories |