Chromosome-scale genome assembly and gene annotation of the Alligator Gar (Atractosteus spatula)

Abstract Given the aggressive nature and robust survival capabilities of the alligator gar (Atractosteus spatula), if it was to exist in a new environment as an invasive species, it could cause significant disruption to the invaded ecosystem. Building on the continuity and completeness of the existi...

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Main Authors: Qing Wang, Qianqian Yu, Xiangqian Dong, Hengjin Chen, Xue Tian, Peng Qi, Haitao Wu, Yuxiang Yuan
Format: Article
Language:English
Published: Nature Portfolio 2024-12-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-024-04256-2
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author Qing Wang
Qianqian Yu
Xiangqian Dong
Hengjin Chen
Xue Tian
Peng Qi
Haitao Wu
Yuxiang Yuan
author_facet Qing Wang
Qianqian Yu
Xiangqian Dong
Hengjin Chen
Xue Tian
Peng Qi
Haitao Wu
Yuxiang Yuan
author_sort Qing Wang
collection DOAJ
description Abstract Given the aggressive nature and robust survival capabilities of the alligator gar (Atractosteus spatula), if it was to exist in a new environment as an invasive species, it could cause significant disruption to the invaded ecosystem. Building on the continuity and completeness of the existing draft genome were not optimal, this study has updated a high-quality genome of the alligator gar at the chromosome level, which was assembled using Oxford Nanopore Technology and chromatin interaction mapping (Hi-C) sequencing techniques. In summary, the alligator gar genome in this study was 1.05 Gb in size with a contig N50 of 15.7 Mb and scaffold N50 of 56.8 Mb. We captured 98.26% of assembled bases in 28 pseudochromosomes. The completeness of the final chromosome-level genome reached 96.7%. Meanwhile, a total of 19,103 protein-coding genes were predicted, of which 99.83% could be predicted with functions. Taken together, the present high-quality alligator gar chromosome-level genome provides a valuable resource for exploring the underlying genomic basis to comprehend the functional genomics, chromosome evolution, and population management of this species.
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institution Kabale University
issn 2052-4463
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spelling doaj-art-14649a106aad46bd85fd4a313e8838f92024-12-22T12:14:05ZengNature PortfolioScientific Data2052-44632024-12-011111810.1038/s41597-024-04256-2Chromosome-scale genome assembly and gene annotation of the Alligator Gar (Atractosteus spatula)Qing Wang0Qianqian Yu1Xiangqian Dong2Hengjin Chen3Xue Tian4Peng Qi5Haitao Wu6Yuxiang Yuan7Key Laboratory of Wetland Ecology and Environment & Heilongjiang Xingkai Lake Wetland Ecosystem National Observation and Research Station, Northeast Institute of Geography and Agroecology, Chinese Academy of SciencesKey Laboratory of Wetland Ecology and Environment & Heilongjiang Xingkai Lake Wetland Ecosystem National Observation and Research Station, Northeast Institute of Geography and Agroecology, Chinese Academy of SciencesKey Laboratory of Wetland Ecology and Environment & Heilongjiang Xingkai Lake Wetland Ecosystem National Observation and Research Station, Northeast Institute of Geography and Agroecology, Chinese Academy of SciencesKey Laboratory of Wetland Ecology and Environment & Heilongjiang Xingkai Lake Wetland Ecosystem National Observation and Research Station, Northeast Institute of Geography and Agroecology, Chinese Academy of SciencesKey Laboratory of Wetland Ecology and Environment & Heilongjiang Xingkai Lake Wetland Ecosystem National Observation and Research Station, Northeast Institute of Geography and Agroecology, Chinese Academy of SciencesKey Laboratory of Wetland Ecology and Environment & Heilongjiang Xingkai Lake Wetland Ecosystem National Observation and Research Station, Northeast Institute of Geography and Agroecology, Chinese Academy of SciencesKey Laboratory of Wetland Ecology and Environment & Heilongjiang Xingkai Lake Wetland Ecosystem National Observation and Research Station, Northeast Institute of Geography and Agroecology, Chinese Academy of SciencesKey Laboratory of Wetland Ecology and Environment & Heilongjiang Xingkai Lake Wetland Ecosystem National Observation and Research Station, Northeast Institute of Geography and Agroecology, Chinese Academy of SciencesAbstract Given the aggressive nature and robust survival capabilities of the alligator gar (Atractosteus spatula), if it was to exist in a new environment as an invasive species, it could cause significant disruption to the invaded ecosystem. Building on the continuity and completeness of the existing draft genome were not optimal, this study has updated a high-quality genome of the alligator gar at the chromosome level, which was assembled using Oxford Nanopore Technology and chromatin interaction mapping (Hi-C) sequencing techniques. In summary, the alligator gar genome in this study was 1.05 Gb in size with a contig N50 of 15.7 Mb and scaffold N50 of 56.8 Mb. We captured 98.26% of assembled bases in 28 pseudochromosomes. The completeness of the final chromosome-level genome reached 96.7%. Meanwhile, a total of 19,103 protein-coding genes were predicted, of which 99.83% could be predicted with functions. Taken together, the present high-quality alligator gar chromosome-level genome provides a valuable resource for exploring the underlying genomic basis to comprehend the functional genomics, chromosome evolution, and population management of this species.https://doi.org/10.1038/s41597-024-04256-2
spellingShingle Qing Wang
Qianqian Yu
Xiangqian Dong
Hengjin Chen
Xue Tian
Peng Qi
Haitao Wu
Yuxiang Yuan
Chromosome-scale genome assembly and gene annotation of the Alligator Gar (Atractosteus spatula)
Scientific Data
title Chromosome-scale genome assembly and gene annotation of the Alligator Gar (Atractosteus spatula)
title_full Chromosome-scale genome assembly and gene annotation of the Alligator Gar (Atractosteus spatula)
title_fullStr Chromosome-scale genome assembly and gene annotation of the Alligator Gar (Atractosteus spatula)
title_full_unstemmed Chromosome-scale genome assembly and gene annotation of the Alligator Gar (Atractosteus spatula)
title_short Chromosome-scale genome assembly and gene annotation of the Alligator Gar (Atractosteus spatula)
title_sort chromosome scale genome assembly and gene annotation of the alligator gar atractosteus spatula
url https://doi.org/10.1038/s41597-024-04256-2
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