Development of selective ssDNA micro-probe for PD1 detection as a novel strategy for cancer imaging
Abstract Programmed death receptor 1, PD1, modulates the function of immune cells by providing inhibitory signals and constitutes the marker of immune exhaustion. Monitoring the level of PD1 promises a useful diagnostic approach in autoimmune diseases and cancer. Here we describe the development of...
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Nature Portfolio
2024-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-74891-7 |
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| author | Stanisław Malicki Anna Czarna Edyta Żyła Barbara Pucelik Wojciech Gałan Barbara Chruścicka Marta Kamińska Alicja Sochaj-Gregorczyk Katarzyna Magiera-Mularz Jun Wang Marek Winiarski Małgorzata Benedyk-Machaczka Joanna Kozieł Grzegorz Dubin Piotr Mydel |
| author_facet | Stanisław Malicki Anna Czarna Edyta Żyła Barbara Pucelik Wojciech Gałan Barbara Chruścicka Marta Kamińska Alicja Sochaj-Gregorczyk Katarzyna Magiera-Mularz Jun Wang Marek Winiarski Małgorzata Benedyk-Machaczka Joanna Kozieł Grzegorz Dubin Piotr Mydel |
| author_sort | Stanisław Malicki |
| collection | DOAJ |
| description | Abstract Programmed death receptor 1, PD1, modulates the function of immune cells by providing inhibitory signals and constitutes the marker of immune exhaustion. Monitoring the level of PD1 promises a useful diagnostic approach in autoimmune diseases and cancer. Here we describe the development of an ssDNA aptamer-based molecular probe capable of specific recognition of human PD1 receptor. The aptamer was selected using SELEX, its sequence was further optimized, and the affinity and specificity were determined in biochemical assays. The aptamer was converted into a fluorescent probe and its potential in molecular imaging was demonstrated in a culture of human cells overexpressing PD1 and murine pancreatic organoids / immune cells mixed co-culture model. We conclude that the provided aptamers are suitable probes for imaging of PD1 expressing immune cells even in complex cellular models and may find future utility as diagnostic tools. |
| format | Article |
| id | doaj-art-143eec6f6a53493c9969dd0d95345919 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-143eec6f6a53493c9969dd0d953459192024-11-24T12:24:11ZengNature PortfolioScientific Reports2045-23222024-11-0114111310.1038/s41598-024-74891-7Development of selective ssDNA micro-probe for PD1 detection as a novel strategy for cancer imagingStanisław Malicki0Anna Czarna1Edyta Żyła2Barbara Pucelik3Wojciech Gałan4Barbara Chruścicka5Marta Kamińska6Alicja Sochaj-Gregorczyk7Katarzyna Magiera-Mularz8Jun Wang9Marek Winiarski10Małgorzata Benedyk-Machaczka11Joanna Kozieł12Grzegorz Dubin13Piotr Mydel14Laboratory of Proteolysis and Post-translational Modification of Proteins, Malopolska Centre of Biotechnology, Jagiellonian UniversityProtein Crystallography Research, Group Malopolska Centre of Biotechnology, Jagiellonian UniversityProtein Crystallography Research, Group Malopolska Centre of Biotechnology, Jagiellonian UniversityProtein Crystallography Research, Group Malopolska Centre of Biotechnology, Jagiellonian UniversityDepartment of Computational Biophysics and Bioinformatics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian UniversityLaboratory of Proteolysis and Post-translational Modification of Proteins, Malopolska Centre of Biotechnology, Jagiellonian UniversityBroegelmann Research Laboratory, University of BergenLaboratory of Proteolysis and Post-translational Modification of Proteins, Malopolska Centre of Biotechnology, Jagiellonian UniversityFaculty of Chemistry, Jagiellonian UniversitySchool of Biomedical Sciences and Engineering, Guangzhou International Campus, South China University of Technology2nd Department of General Surgery, Faculty of Medicine, Jagiellonian University Medical CollegeDepartment of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian UniversityDepartment of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian UniversityProtein Crystallography Research, Group Malopolska Centre of Biotechnology, Jagiellonian UniversityDepartment of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian UniversityAbstract Programmed death receptor 1, PD1, modulates the function of immune cells by providing inhibitory signals and constitutes the marker of immune exhaustion. Monitoring the level of PD1 promises a useful diagnostic approach in autoimmune diseases and cancer. Here we describe the development of an ssDNA aptamer-based molecular probe capable of specific recognition of human PD1 receptor. The aptamer was selected using SELEX, its sequence was further optimized, and the affinity and specificity were determined in biochemical assays. The aptamer was converted into a fluorescent probe and its potential in molecular imaging was demonstrated in a culture of human cells overexpressing PD1 and murine pancreatic organoids / immune cells mixed co-culture model. We conclude that the provided aptamers are suitable probes for imaging of PD1 expressing immune cells even in complex cellular models and may find future utility as diagnostic tools.https://doi.org/10.1038/s41598-024-74891-7 |
| spellingShingle | Stanisław Malicki Anna Czarna Edyta Żyła Barbara Pucelik Wojciech Gałan Barbara Chruścicka Marta Kamińska Alicja Sochaj-Gregorczyk Katarzyna Magiera-Mularz Jun Wang Marek Winiarski Małgorzata Benedyk-Machaczka Joanna Kozieł Grzegorz Dubin Piotr Mydel Development of selective ssDNA micro-probe for PD1 detection as a novel strategy for cancer imaging Scientific Reports |
| title | Development of selective ssDNA micro-probe for PD1 detection as a novel strategy for cancer imaging |
| title_full | Development of selective ssDNA micro-probe for PD1 detection as a novel strategy for cancer imaging |
| title_fullStr | Development of selective ssDNA micro-probe for PD1 detection as a novel strategy for cancer imaging |
| title_full_unstemmed | Development of selective ssDNA micro-probe for PD1 detection as a novel strategy for cancer imaging |
| title_short | Development of selective ssDNA micro-probe for PD1 detection as a novel strategy for cancer imaging |
| title_sort | development of selective ssdna micro probe for pd1 detection as a novel strategy for cancer imaging |
| url | https://doi.org/10.1038/s41598-024-74891-7 |
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