Identification and characterisation of colistin-resistant Acinetobacter colistiniresistens co-producing IMP-1 and OXA-58 carbapenemases
Background: Carbapenem-resistant Acinetobacter is of increasing global concern because infections are challenging to treat with standard antibiotics. Here, we identified a previously uncharacterised Acinetobacter sp. clinical isolate as Acinetobacter colistiniresistens co-producing IMP-1 and OXA-58....
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Elsevier
2024-12-01
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| Series: | New Microbes and New Infections |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2052297524002683 |
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| author | Satoshi Nishida Yasuo Ono |
| author_facet | Satoshi Nishida Yasuo Ono |
| author_sort | Satoshi Nishida |
| collection | DOAJ |
| description | Background: Carbapenem-resistant Acinetobacter is of increasing global concern because infections are challenging to treat with standard antibiotics. Here, we identified a previously uncharacterised Acinetobacter sp. clinical isolate as Acinetobacter colistiniresistens co-producing IMP-1 and OXA-58. We also examined expression of genes related to antibiotic susceptibility and drug resistance, including blaIMP. Methods: The isolate was deposited at the National Institute of Technology and Evaluation (NITE) as Acinetobacter sp. NBRC 110496. Susceptibility was defined according to the Clinical and Laboratory Standards Institute (CLSI) breakpoints. Genomic and clonal analyses were performed to identify species and resistance genes. Results: The isolate was resistant to β-lactams, including broad-spectrum cephalosporins and carbapenems, polymyxins, and trimethoprim/sulfamethoxazole. Genomic analysis identified the isolate as A. colistiniresistens harbouring blaIMP-1, blaOXA-58, blaOXA-670, aac(6′)-Ib, aac(6′)-Ij, ant(3”)-II, aph(3’)-VI, msrE, mphE, and sul1. Colistin resistance was associated with the eptA-like gene, which encodes a lipid A-modifying enzyme. SNP-based phylogenetic analysis revealed that the strain clustered with other strains isolated in Japan. The IMP-1/OXA-58-producing strain described in this study has a novel integron structure surrounding blaIMP-1, aacA and sul1. Conclusions: Colistin-resistant IMP-1/OXA-58-co-producing A. colistiniresistens was identified in a patient. This isolate could serve as a reservoir for carbapenemase-producing organisms. This study suggests that screening for colistin-resistant isolates is crucial to preserve colistin as a therapeutic agent for multidrug-resistant bacteria. Identification of this MDR isolate in Asia, and the danger of it spreading worldwide, should raise serious concerns. |
| format | Article |
| id | doaj-art-13f9d5167bd64cf5938a1cc0cda6fbb0 |
| institution | Kabale University |
| issn | 2052-2975 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | New Microbes and New Infections |
| spelling | doaj-art-13f9d5167bd64cf5938a1cc0cda6fbb02024-12-13T10:57:17ZengElsevierNew Microbes and New Infections2052-29752024-12-0162101484Identification and characterisation of colistin-resistant Acinetobacter colistiniresistens co-producing IMP-1 and OXA-58 carbapenemasesSatoshi Nishida0Yasuo Ono1Department of Microbiology and Immunology, Teikyo University School of Medicine, Kaga, Itabashi, Tokyo, Japan; Corresponding author. Department of Microbiology and Immunology, Teikyo University School of Medicine, Itabashi, Tokyo, 173-8605, Japan.Department of Microbiology and Immunology, Teikyo University School of Medicine, Kaga, Itabashi, Tokyo, Japan; Faculty of Health and Medical Science, Teikyo Heisei University, Higashi-Ikebukuro, Toshima, Tokyo, JapanBackground: Carbapenem-resistant Acinetobacter is of increasing global concern because infections are challenging to treat with standard antibiotics. Here, we identified a previously uncharacterised Acinetobacter sp. clinical isolate as Acinetobacter colistiniresistens co-producing IMP-1 and OXA-58. We also examined expression of genes related to antibiotic susceptibility and drug resistance, including blaIMP. Methods: The isolate was deposited at the National Institute of Technology and Evaluation (NITE) as Acinetobacter sp. NBRC 110496. Susceptibility was defined according to the Clinical and Laboratory Standards Institute (CLSI) breakpoints. Genomic and clonal analyses were performed to identify species and resistance genes. Results: The isolate was resistant to β-lactams, including broad-spectrum cephalosporins and carbapenems, polymyxins, and trimethoprim/sulfamethoxazole. Genomic analysis identified the isolate as A. colistiniresistens harbouring blaIMP-1, blaOXA-58, blaOXA-670, aac(6′)-Ib, aac(6′)-Ij, ant(3”)-II, aph(3’)-VI, msrE, mphE, and sul1. Colistin resistance was associated with the eptA-like gene, which encodes a lipid A-modifying enzyme. SNP-based phylogenetic analysis revealed that the strain clustered with other strains isolated in Japan. The IMP-1/OXA-58-producing strain described in this study has a novel integron structure surrounding blaIMP-1, aacA and sul1. Conclusions: Colistin-resistant IMP-1/OXA-58-co-producing A. colistiniresistens was identified in a patient. This isolate could serve as a reservoir for carbapenemase-producing organisms. This study suggests that screening for colistin-resistant isolates is crucial to preserve colistin as a therapeutic agent for multidrug-resistant bacteria. Identification of this MDR isolate in Asia, and the danger of it spreading worldwide, should raise serious concerns.http://www.sciencedirect.com/science/article/pii/S2052297524002683Acinetobacter colistiniresistensCarbapenemCarbapenemaseColistinIMP-1MBL |
| spellingShingle | Satoshi Nishida Yasuo Ono Identification and characterisation of colistin-resistant Acinetobacter colistiniresistens co-producing IMP-1 and OXA-58 carbapenemases New Microbes and New Infections Acinetobacter colistiniresistens Carbapenem Carbapenemase Colistin IMP-1 MBL |
| title | Identification and characterisation of colistin-resistant Acinetobacter colistiniresistens co-producing IMP-1 and OXA-58 carbapenemases |
| title_full | Identification and characterisation of colistin-resistant Acinetobacter colistiniresistens co-producing IMP-1 and OXA-58 carbapenemases |
| title_fullStr | Identification and characterisation of colistin-resistant Acinetobacter colistiniresistens co-producing IMP-1 and OXA-58 carbapenemases |
| title_full_unstemmed | Identification and characterisation of colistin-resistant Acinetobacter colistiniresistens co-producing IMP-1 and OXA-58 carbapenemases |
| title_short | Identification and characterisation of colistin-resistant Acinetobacter colistiniresistens co-producing IMP-1 and OXA-58 carbapenemases |
| title_sort | identification and characterisation of colistin resistant acinetobacter colistiniresistens co producing imp 1 and oxa 58 carbapenemases |
| topic | Acinetobacter colistiniresistens Carbapenem Carbapenemase Colistin IMP-1 MBL |
| url | http://www.sciencedirect.com/science/article/pii/S2052297524002683 |
| work_keys_str_mv | AT satoshinishida identificationandcharacterisationofcolistinresistantacinetobactercolistiniresistenscoproducingimp1andoxa58carbapenemases AT yasuoono identificationandcharacterisationofcolistinresistantacinetobactercolistiniresistenscoproducingimp1andoxa58carbapenemases |