Efficacy of Air and Oxygen Micro-nano Bubble Waters Against Colletotrichum gloeosporioides and Impacts on Postharvest Quality of ‘Fan Retief’ Guava Fruit
This study focused on the application of micro-nano bubbles (MNBs) water generated using air or oxygen (O2), as an alternative to chlorine-based wash for fruits. For the in vitro and in vivo investigation, 106 spore or conidia/mL Colletotrichum gloeosporioides suspension was used, and treated with s...
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Elsevier
2025-01-01
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| Series: | Journal of Food Protection |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0362028X24002217 |
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| author | Harold K. Malahlela Zinash A. Belay Rebogile R. Mphahlele Oluwafemi James Caleb |
| author_facet | Harold K. Malahlela Zinash A. Belay Rebogile R. Mphahlele Oluwafemi James Caleb |
| author_sort | Harold K. Malahlela |
| collection | DOAJ |
| description | This study focused on the application of micro-nano bubbles (MNBs) water generated using air or oxygen (O2), as an alternative to chlorine-based wash for fruits. For the in vitro and in vivo investigation, 106 spore or conidia/mL Colletotrichum gloeosporioides suspension was used, and treated with solutions of air- or O2-MNB for 30- or 60-min, sodium hypochlorite (NaOCl), and untreated (as control). In the second experiment, freshly harvested guava fruits were washed with tap water (control), NaOCl (standard practice), air-, or O2-MNB (for 15- or 30-min). All samples were packaged, stored for 21 days at 13 °C, and monitored for changes in natural microbial population and quality attributes. Based on the confocal laser and transmission electron microscopy results, exposure of C. gloeosporioides to air-MNB for 60 min resulted in the lowest viable cell count (%) compared to control and other treatments (O2-MNB and NaOCl). Air- and O2-MNB treatments damaged cellular structures, disrupted cell membrane integrity, and deformed hyphal morphology. Washing ‘Fan Retief’ guava (Psidium guajava L.) in air- or O2-MNB (for 15 and/or 30 min), better-retained tissue strength, delayed changes in color, and total soluble solid (TSS) content. Notably, MNB treatments were as effective as NaOCl washing and significantly reduced microbial load on fruit surface by ≥2 Log (p < 0.05). Micro-nano bubble water treatment offers a new paradigm for decontamination and preservation of guava fruit quality. |
| format | Article |
| id | doaj-art-1356f66bc0b748688325391c014dfe70 |
| institution | Kabale University |
| issn | 0362-028X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Food Protection |
| spelling | doaj-art-1356f66bc0b748688325391c014dfe702025-01-09T06:12:40ZengElsevierJournal of Food Protection0362-028X2025-01-01881100437Efficacy of Air and Oxygen Micro-nano Bubble Waters Against Colletotrichum gloeosporioides and Impacts on Postharvest Quality of ‘Fan Retief’ Guava FruitHarold K. Malahlela0Zinash A. Belay1Rebogile R. Mphahlele2Oluwafemi James Caleb3Department of Food Science, Faculty of AgriSciences, Stellenbosch University, Matieland, South Africa; AgriFood BioSystems and Technovation Research Group, Africa Institute for Postharvest Technology, Faculty of AgriSciences, Stellenbosch University, Matieland, South AfricaAgri-Food Systems and Omics Laboratory, Post-Harvest and Agro-Processing Technologies (PHATs), Agricultural Research Council (ARC) Infruitec-Nietvoorbij, Stellenbosch, South AfricaDepartment of Land Reform and Rural Development, Private Bag X250, Pretoria, 0001, South AfricaDepartment of Food Science, Faculty of AgriSciences, Stellenbosch University, Matieland, South Africa; AgriFood BioSystems and Technovation Research Group, Africa Institute for Postharvest Technology, Faculty of AgriSciences, Stellenbosch University, Matieland, South Africa; Corresponding author at: AgriFood BioSystems and Technovation Research Group, Africa Institute for Postharvest Technology, Faculty of AgriSciences, Stellenbosch University, Matieland, South Africa.This study focused on the application of micro-nano bubbles (MNBs) water generated using air or oxygen (O2), as an alternative to chlorine-based wash for fruits. For the in vitro and in vivo investigation, 106 spore or conidia/mL Colletotrichum gloeosporioides suspension was used, and treated with solutions of air- or O2-MNB for 30- or 60-min, sodium hypochlorite (NaOCl), and untreated (as control). In the second experiment, freshly harvested guava fruits were washed with tap water (control), NaOCl (standard practice), air-, or O2-MNB (for 15- or 30-min). All samples were packaged, stored for 21 days at 13 °C, and monitored for changes in natural microbial population and quality attributes. Based on the confocal laser and transmission electron microscopy results, exposure of C. gloeosporioides to air-MNB for 60 min resulted in the lowest viable cell count (%) compared to control and other treatments (O2-MNB and NaOCl). Air- and O2-MNB treatments damaged cellular structures, disrupted cell membrane integrity, and deformed hyphal morphology. Washing ‘Fan Retief’ guava (Psidium guajava L.) in air- or O2-MNB (for 15 and/or 30 min), better-retained tissue strength, delayed changes in color, and total soluble solid (TSS) content. Notably, MNB treatments were as effective as NaOCl washing and significantly reduced microbial load on fruit surface by ≥2 Log (p < 0.05). Micro-nano bubble water treatment offers a new paradigm for decontamination and preservation of guava fruit quality.http://www.sciencedirect.com/science/article/pii/S0362028X24002217Activated water systemsConfocal laser scanning microscopyIntracellular structuresMicro-nano bubbleProduce qualityPsidium guajava L. |
| spellingShingle | Harold K. Malahlela Zinash A. Belay Rebogile R. Mphahlele Oluwafemi James Caleb Efficacy of Air and Oxygen Micro-nano Bubble Waters Against Colletotrichum gloeosporioides and Impacts on Postharvest Quality of ‘Fan Retief’ Guava Fruit Journal of Food Protection Activated water systems Confocal laser scanning microscopy Intracellular structures Micro-nano bubble Produce quality Psidium guajava L. |
| title | Efficacy of Air and Oxygen Micro-nano Bubble Waters Against Colletotrichum gloeosporioides and Impacts on Postharvest Quality of ‘Fan Retief’ Guava Fruit |
| title_full | Efficacy of Air and Oxygen Micro-nano Bubble Waters Against Colletotrichum gloeosporioides and Impacts on Postharvest Quality of ‘Fan Retief’ Guava Fruit |
| title_fullStr | Efficacy of Air and Oxygen Micro-nano Bubble Waters Against Colletotrichum gloeosporioides and Impacts on Postharvest Quality of ‘Fan Retief’ Guava Fruit |
| title_full_unstemmed | Efficacy of Air and Oxygen Micro-nano Bubble Waters Against Colletotrichum gloeosporioides and Impacts on Postharvest Quality of ‘Fan Retief’ Guava Fruit |
| title_short | Efficacy of Air and Oxygen Micro-nano Bubble Waters Against Colletotrichum gloeosporioides and Impacts on Postharvest Quality of ‘Fan Retief’ Guava Fruit |
| title_sort | efficacy of air and oxygen micro nano bubble waters against colletotrichum gloeosporioides and impacts on postharvest quality of fan retief guava fruit |
| topic | Activated water systems Confocal laser scanning microscopy Intracellular structures Micro-nano bubble Produce quality Psidium guajava L. |
| url | http://www.sciencedirect.com/science/article/pii/S0362028X24002217 |
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