A new and promising C-phycocyanin-producing cyanobacterial strain, Cyanobium sp. MMK01: practical strategy towards developing a methodology to achieve C-phycocyanin with ultra-high purity
Selecting a suitable cyanobacterial strain and developing easy-to-afford purification processes are two crucial aspects impacting the optimal production yield and appropriate purity of C-phycocyanin (C-PC). Cyanobium sp. MMK01, a highly efficient C-PC-producing bacterium, was identified among four c...
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1394617/full |
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| author | Melika Shafiei Maral Shafiei Naeema Mohseni Sani Wangbiao Guo Shuaiqi Guo Hojatollah Vali Kambiz Akbari Noghabi |
| author_facet | Melika Shafiei Maral Shafiei Naeema Mohseni Sani Wangbiao Guo Shuaiqi Guo Hojatollah Vali Kambiz Akbari Noghabi |
| author_sort | Melika Shafiei |
| collection | DOAJ |
| description | Selecting a suitable cyanobacterial strain and developing easy-to-afford purification processes are two crucial aspects impacting the optimal production yield and appropriate purity of C-phycocyanin (C-PC). Cyanobium sp. MMK01, a highly efficient C-PC-producing bacterium, was identified among four cyanobacterial isolates using morphological characteristics and 16S rRNA gene sequencing. The purification process of C-PC began with ammonium sulfate precipitation, leading to a purity index (PI) of 4.04. Subsequent purification through ion exchange chromatography ultimately resulted in an ultra-highly purified form of C-PC with a significant PI of 5.82. SDS-PAGE analysis of purified C-PC showed the presence of two distinct bands, α (13 kDa) and β (15 kDa). Significantly effective at scavenging free radicals, C-PC also inhibits the viability of human lung cancer cells (Calu-6). Antibacterial, anti-inflammatory, antioxidant, and cancer-preventive compounds were detected in the MMK01 cells’ methanolic extract following GC–MS analysis. The promising results indicate that Cyanobium sp. MMK01 has a great deal of potential for producing C-PC that is on par with strains found in the market, and the tried-and-true two-step purification process proved to work well to achieve an ultra-highly purified form of C-PC. |
| format | Article |
| id | doaj-art-132e42fc74bf4c79a77f60da3b1b4c99 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-132e42fc74bf4c79a77f60da3b1b4c992024-11-21T06:23:47ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-11-011510.3389/fmicb.2024.13946171394617A new and promising C-phycocyanin-producing cyanobacterial strain, Cyanobium sp. MMK01: practical strategy towards developing a methodology to achieve C-phycocyanin with ultra-high purityMelika Shafiei0Maral Shafiei1Naeema Mohseni Sani2Wangbiao Guo3Shuaiqi Guo4Hojatollah Vali5Kambiz Akbari Noghabi6Department of Energy and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, IranDepartment of Energy and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, IranDepartment of Energy and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, IranSchool of Medicine, Yale University, West Haven, CT, United StatesDepartment of Anatomy & Cell Biology, McGill University, Montreal, QC, CanadaDepartment of Anatomy & Cell Biology, McGill University, Montreal, QC, CanadaDepartment of Energy and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, IranSelecting a suitable cyanobacterial strain and developing easy-to-afford purification processes are two crucial aspects impacting the optimal production yield and appropriate purity of C-phycocyanin (C-PC). Cyanobium sp. MMK01, a highly efficient C-PC-producing bacterium, was identified among four cyanobacterial isolates using morphological characteristics and 16S rRNA gene sequencing. The purification process of C-PC began with ammonium sulfate precipitation, leading to a purity index (PI) of 4.04. Subsequent purification through ion exchange chromatography ultimately resulted in an ultra-highly purified form of C-PC with a significant PI of 5.82. SDS-PAGE analysis of purified C-PC showed the presence of two distinct bands, α (13 kDa) and β (15 kDa). Significantly effective at scavenging free radicals, C-PC also inhibits the viability of human lung cancer cells (Calu-6). Antibacterial, anti-inflammatory, antioxidant, and cancer-preventive compounds were detected in the MMK01 cells’ methanolic extract following GC–MS analysis. The promising results indicate that Cyanobium sp. MMK01 has a great deal of potential for producing C-PC that is on par with strains found in the market, and the tried-and-true two-step purification process proved to work well to achieve an ultra-highly purified form of C-PC.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1394617/fullCyanobium sp. MMK01C-phycocyaninion-exchange chromatographyhuman lung cancer cells (Calu-6)MTT assay |
| spellingShingle | Melika Shafiei Maral Shafiei Naeema Mohseni Sani Wangbiao Guo Shuaiqi Guo Hojatollah Vali Kambiz Akbari Noghabi A new and promising C-phycocyanin-producing cyanobacterial strain, Cyanobium sp. MMK01: practical strategy towards developing a methodology to achieve C-phycocyanin with ultra-high purity Frontiers in Microbiology Cyanobium sp. MMK01 C-phycocyanin ion-exchange chromatography human lung cancer cells (Calu-6) MTT assay |
| title | A new and promising C-phycocyanin-producing cyanobacterial strain, Cyanobium sp. MMK01: practical strategy towards developing a methodology to achieve C-phycocyanin with ultra-high purity |
| title_full | A new and promising C-phycocyanin-producing cyanobacterial strain, Cyanobium sp. MMK01: practical strategy towards developing a methodology to achieve C-phycocyanin with ultra-high purity |
| title_fullStr | A new and promising C-phycocyanin-producing cyanobacterial strain, Cyanobium sp. MMK01: practical strategy towards developing a methodology to achieve C-phycocyanin with ultra-high purity |
| title_full_unstemmed | A new and promising C-phycocyanin-producing cyanobacterial strain, Cyanobium sp. MMK01: practical strategy towards developing a methodology to achieve C-phycocyanin with ultra-high purity |
| title_short | A new and promising C-phycocyanin-producing cyanobacterial strain, Cyanobium sp. MMK01: practical strategy towards developing a methodology to achieve C-phycocyanin with ultra-high purity |
| title_sort | new and promising c phycocyanin producing cyanobacterial strain cyanobium sp mmk01 practical strategy towards developing a methodology to achieve c phycocyanin with ultra high purity |
| topic | Cyanobium sp. MMK01 C-phycocyanin ion-exchange chromatography human lung cancer cells (Calu-6) MTT assay |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1394617/full |
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