Changes in CD4+CD25HIGH T cells and TGFb1 levels in different stages of adult-onset type 1 diabetes
Background: Previous studies suggested an important role of impairments in T cell subsets in different stages during type 1 diabetes (T1D) development, while data regarding CD25high T cells and transforming growth factor b1 (TGFb1), both T regulatory associated, remains controversial. We analyzed th...
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Society of Medical Biochemists of Serbia, Belgrade
2024-01-01
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| Series: | Journal of Medical Biochemistry |
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| author | Miličić Tanja Jotić Aleksandra Marković Ivanka Popadić Dušan Lalić Katarina Uskoković Veljko Lukić Ljiljana Maćešić Marija Stanarčić Jelena Stoiljković Milica Milovančević Mina Rafailović Đurđa Božović Aleksandra Radisavljević Nina Lalić Nebojša M. |
| author_facet | Miličić Tanja Jotić Aleksandra Marković Ivanka Popadić Dušan Lalić Katarina Uskoković Veljko Lukić Ljiljana Maćešić Marija Stanarčić Jelena Stoiljković Milica Milovančević Mina Rafailović Đurđa Božović Aleksandra Radisavljević Nina Lalić Nebojša M. |
| author_sort | Miličić Tanja |
| collection | DOAJ |
| description | Background: Previous studies suggested an important role of impairments in T cell subsets in different stages during type 1 diabetes (T1D) development, while data regarding CD25high T cells and transforming growth factor b1 (TGFb1), both T regulatory associated, remains controversial. We analyzed the level of (a) CD25high T cells (b) TGFb1 in 17 first-degree relatives of patients with T1D in stage 1 (FDRs1) (GADA+, IA-2+); 34 FDRs in stage 0 (FDRs0) (GADA, IA-2); 24 recent-onset T1D in insulin-requiring state (IRS); 10 patients in clinical remission (CR); 18 healthy, unrelated controls (CTR). Methods: T cell subsets were characterized by two-color immunofluorescence staining and flow cytometry; TGFb1 was determined by ELISA, GADA, and IA-2 by RIA. Results: The percentage of CD25high T cells in FDRs1 was lower than controls, FDRs0, IRS, and CR (p<0.001). Additionally, the cut-off value for CD25high = 1.19%, with a probability of 0.667, for having a higher risk for T1D. TGFb1 concentration in FDRs1, FDRs0, IRS, and CR, was lower than controls (p<0.001). IRS has a higher TGFb1 concentration than CR (p<0.001). Conclusions: Stage 1, a higher risk for T1D, is characterized by decreases in CD25high T cells and TGFb1, partially reflecting impaired T regulatory response, implying that changes of this T cells subset might be a risk marker for T1D. FDRs, irrespective of risk for T1D and T1D patients irrespective of state, had depletion of TGFb1, suggesting the association of TGFb1 could have potential with familiar risk and manifestation of T1D. Furthermore, the result suggested that the clinical course of overt T1D might be modulated on the TGFb1 level. |
| format | Article |
| id | doaj-art-1284c93e8b3242b2ae740ea4358f2920 |
| institution | Kabale University |
| issn | 1452-8258 1452-8266 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Society of Medical Biochemists of Serbia, Belgrade |
| record_format | Article |
| series | Journal of Medical Biochemistry |
| spelling | doaj-art-1284c93e8b3242b2ae740ea4358f29202024-12-02T12:53:03ZengSociety of Medical Biochemists of Serbia, BelgradeJournal of Medical Biochemistry1452-82581452-82662024-01-0143691592610.5937/jomb0-498681452-82582406915MChanges in CD4+CD25HIGH T cells and TGFb1 levels in different stages of adult-onset type 1 diabetesMiličić Tanja0https://orcid.org/0000-0001-7300-916XJotić Aleksandra1https://orcid.org/0000-0002-7997-9076Marković Ivanka2https://orcid.org/0000-0002-7961-3752Popadić Dušan3https://orcid.org/0000-0002-7502-1709Lalić Katarina4https://orcid.org/0000-0002-8070-1899Uskoković Veljko5https://orcid.org/0009-0004-9760-4472Lukić Ljiljana6https://orcid.org/0000-0002-3513-4434Maćešić Marija7https://orcid.org/0000-0001-9656-2523Stanarčić Jelena8https://orcid.org/0009-0007-2969-1852Stoiljković Milica9https://orcid.org/0009-0000-1322-0107Milovančević Mina10https://orcid.org/0000-0002-0537-8139Rafailović Đurđa11Božović Aleksandra12Radisavljević Nina13Lalić Nebojša M.14https://orcid.org/0000-0002-8082-6560University of Belgrade, Faculty of Medicine, University Clinical Centre of Serbia, Clinic for Endocrinology, Diabetes and Metabolic Diseases, Belgrade, SerbiaUniversity of Belgrade, Faculty of Medicine, University Clinical Centre of Serbia, Clinic for Endocrinology, Diabetes and Metabolic Diseases, Belgrade, SerbiaUniversity of Belgrade, Faculty of Medicine, Institute for Medical and Clinical Biochemistry, Belgrade, SerbiaUniversity of Belgrade, Faculty of Medicine, Institute for Microbiology and Immunology, Belgrade, SerbiaUniversity of Belgrade, Faculty of Medicine, University Clinical Centre of Serbia, Clinic for Endocrinology, Diabetes and Metabolic Diseases, Belgrade, SerbiaUniversity of Belgrade, Faculty of Organizational Sciences, Department for Operations Research and Statistics, Belgrade, SerbiaUniversity of Belgrade, Faculty of Medicine, University Clinical Centre of Serbia, Clinic for Endocrinology, Diabetes and Metabolic Diseases, Belgrade, SerbiaUniversity of Belgrade, Faculty of Medicine, University Clinical Centre of Serbia, Clinic for Endocrinology, Diabetes and Metabolic Diseases, Belgrade, SerbiaUniversity of Belgrade, Faculty of Medicine, University Clinical Centre of Serbia, Clinic for Endocrinology, Diabetes and Metabolic Diseases, Belgrade, SerbiaUniversity of Belgrade, Faculty of Medicine, University Clinical Centre of Serbia, Clinic for Endocrinology, Diabetes and Metabolic Diseases, Belgrade, SerbiaUniversity of Belgrade, Faculty of Medicine, University Clinical Centre of Serbia, Clinic for Endocrinology, Diabetes and Metabolic Diseases, Belgrade, SerbiaUniversity of Belgrade, Faculty of Medicine, University Clinical Centre of Serbia, Clinic for Endocrinology, Diabetes and Metabolic Diseases, Belgrade, SerbiaUniversity of Belgrade, Faculty of Medicine, University Clinical Centre of Serbia, Clinic for Endocrinology, Diabetes and Metabolic Diseases, Belgrade, SerbiaUniversity of Belgrade, Faculty of Medicine, University Clinical Centre of Serbia, Clinic for Endocrinology, Diabetes and Metabolic Diseases, Belgrade, SerbiaUniversity of Belgrade, Faculty of Medicine, University Clinical Centre of Serbia, Clinic for Endocrinology, Diabetes and Metabolic Diseases, Belgrade, SerbiaBackground: Previous studies suggested an important role of impairments in T cell subsets in different stages during type 1 diabetes (T1D) development, while data regarding CD25high T cells and transforming growth factor b1 (TGFb1), both T regulatory associated, remains controversial. We analyzed the level of (a) CD25high T cells (b) TGFb1 in 17 first-degree relatives of patients with T1D in stage 1 (FDRs1) (GADA+, IA-2+); 34 FDRs in stage 0 (FDRs0) (GADA, IA-2); 24 recent-onset T1D in insulin-requiring state (IRS); 10 patients in clinical remission (CR); 18 healthy, unrelated controls (CTR). Methods: T cell subsets were characterized by two-color immunofluorescence staining and flow cytometry; TGFb1 was determined by ELISA, GADA, and IA-2 by RIA. Results: The percentage of CD25high T cells in FDRs1 was lower than controls, FDRs0, IRS, and CR (p<0.001). Additionally, the cut-off value for CD25high = 1.19%, with a probability of 0.667, for having a higher risk for T1D. TGFb1 concentration in FDRs1, FDRs0, IRS, and CR, was lower than controls (p<0.001). IRS has a higher TGFb1 concentration than CR (p<0.001). Conclusions: Stage 1, a higher risk for T1D, is characterized by decreases in CD25high T cells and TGFb1, partially reflecting impaired T regulatory response, implying that changes of this T cells subset might be a risk marker for T1D. FDRs, irrespective of risk for T1D and T1D patients irrespective of state, had depletion of TGFb1, suggesting the association of TGFb1 could have potential with familiar risk and manifestation of T1D. Furthermore, the result suggested that the clinical course of overt T1D might be modulated on the TGFb1 level.https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2024/1452-82582406915M.pdfcd4+cd25high t cellsfirst-degree relatives of patients with type 1 diabetestgfbtype 1 diabetes |
| spellingShingle | Miličić Tanja Jotić Aleksandra Marković Ivanka Popadić Dušan Lalić Katarina Uskoković Veljko Lukić Ljiljana Maćešić Marija Stanarčić Jelena Stoiljković Milica Milovančević Mina Rafailović Đurđa Božović Aleksandra Radisavljević Nina Lalić Nebojša M. Changes in CD4+CD25HIGH T cells and TGFb1 levels in different stages of adult-onset type 1 diabetes Journal of Medical Biochemistry cd4+cd25high t cells first-degree relatives of patients with type 1 diabetes tgfb type 1 diabetes |
| title | Changes in CD4+CD25HIGH T cells and TGFb1 levels in different stages of adult-onset type 1 diabetes |
| title_full | Changes in CD4+CD25HIGH T cells and TGFb1 levels in different stages of adult-onset type 1 diabetes |
| title_fullStr | Changes in CD4+CD25HIGH T cells and TGFb1 levels in different stages of adult-onset type 1 diabetes |
| title_full_unstemmed | Changes in CD4+CD25HIGH T cells and TGFb1 levels in different stages of adult-onset type 1 diabetes |
| title_short | Changes in CD4+CD25HIGH T cells and TGFb1 levels in different stages of adult-onset type 1 diabetes |
| title_sort | changes in cd4 cd25high t cells and tgfb1 levels in different stages of adult onset type 1 diabetes |
| topic | cd4+cd25high t cells first-degree relatives of patients with type 1 diabetes tgfb type 1 diabetes |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2024/1452-82582406915M.pdf |
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