Profiling the gut structure and microbiota, and identifying two dominant bacteria belonging to the Weissella genus in mandarin fish (Siniperca chuatsi) fed an artificial diet
Mandarin fish (Siniperca chuatsi) fed with an artificial diet is progressively gaining popularity, which is important for reducing product prices and resource consumption. However, food is the decisive factor of intestinal microbes, and the profound effects of change in their feeding habit on intest...
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1486501/full |
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| author | Jiayu Wang Jiayu Wang Yaotong Hao Yaotong Hao Lihan Zhang Lihan Zhang Xiaowei Gao Xiaowei Gao Yihuan Xu Yihuan Xu Jiangjiang Wang Jiangjiang Wang Fazhan Hanafiah Waiho Khor Yanfeng Sun Yanfeng Sun Chengbin Wu Chengbin Wu |
| author_facet | Jiayu Wang Jiayu Wang Yaotong Hao Yaotong Hao Lihan Zhang Lihan Zhang Xiaowei Gao Xiaowei Gao Yihuan Xu Yihuan Xu Jiangjiang Wang Jiangjiang Wang Fazhan Hanafiah Waiho Khor Yanfeng Sun Yanfeng Sun Chengbin Wu Chengbin Wu |
| author_sort | Jiayu Wang |
| collection | DOAJ |
| description | Mandarin fish (Siniperca chuatsi) fed with an artificial diet is progressively gaining popularity, which is important for reducing product prices and resource consumption. However, food is the decisive factor of intestinal microbes, and the profound effects of change in their feeding habit on intestinal microbes of mandarin fish have not been revealed. In the present study, live bait fish and artificial diet were used to feed mandarin fish for 8 weeks to study the effect of different feeding habits on the histology, microbiota structure and dominant bacteria of gut in mandarin fish. The results indicated that feeding with the artificial diet significantly increased the intestinal villi height and muscular thickness in the hindgut of mandarin fish. In addition, the microbiota results showed that there were significant differences of beta diversity of gut microbiota in mandarin fish fed with different diets. At the genus level, feeding artificial diets significantly increased the abundance of Weissella in the mandarin fish gut. Furthermore, two Weissella bacteria were identified and characterized from the midgut contents of mandarin fish fed with artificial diet. Based on 16S rRNA gene sequencing, nine strains were assigned as Weissella confusa (RM125), and one as Weissella cibaria (SJ548). Biochemical analyses based on the VITEK2 method revealed a pattern of metabolic activities against W. confusa RM125 and W. cibaria SJ548, with 13 positive and 29 negative results, respectively. W. confusa RM125 and W. cibaria SJ548 exhibited sensitivities toward a variety of pathogens, including V. harveyi, S. aureus and V. parahaemolyticus, E. coli, A. hydrophila, S. enterica, V. anguillarum, and V. alginolyticus, indicating potential probiotics. Therefore, our results confirmed that the transformation of feeding habit altered the structure, microbial composition and dominant bacteria in gut of mandarin fish, and provided evidence that Weissella might play a crucial role in the digestion and absorption of artificial diet in mandarin fish. |
| format | Article |
| id | doaj-art-1235f47d50814b91a95c9b839e1d56b5 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-1235f47d50814b91a95c9b839e1d56b52024-11-29T16:44:40ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-11-011510.3389/fmicb.2024.14865011486501Profiling the gut structure and microbiota, and identifying two dominant bacteria belonging to the Weissella genus in mandarin fish (Siniperca chuatsi) fed an artificial dietJiayu Wang0Jiayu Wang1Yaotong Hao2Yaotong Hao3Lihan Zhang4Lihan Zhang5Xiaowei Gao6Xiaowei Gao7Yihuan Xu8Yihuan Xu9Jiangjiang Wang10Jiangjiang Wang11Fazhan Hanafiah12Waiho Khor13Yanfeng Sun14Yanfeng Sun15Chengbin Wu16Chengbin Wu17Ocean College, Hebei Agricultural University, Qinhuangdao, Hebei, ChinaHebei Key Laboratory of Nutritional Regulation and Disease Control for Aquaculture, Qinhuangdao, Hebei, ChinaOcean College, Hebei Agricultural University, Qinhuangdao, Hebei, ChinaHebei Key Laboratory of Nutritional Regulation and Disease Control for Aquaculture, Qinhuangdao, Hebei, ChinaOcean College, Hebei Agricultural University, Qinhuangdao, Hebei, ChinaHebei Key Laboratory of Nutritional Regulation and Disease Control for Aquaculture, Qinhuangdao, Hebei, ChinaOcean College, Hebei Agricultural University, Qinhuangdao, Hebei, ChinaHebei Key Laboratory of Nutritional Regulation and Disease Control for Aquaculture, Qinhuangdao, Hebei, ChinaOcean College, Hebei Agricultural University, Qinhuangdao, Hebei, ChinaHebei Key Laboratory of Nutritional Regulation and Disease Control for Aquaculture, Qinhuangdao, Hebei, ChinaOcean College, Hebei Agricultural University, Qinhuangdao, Hebei, ChinaHebei Key Laboratory of Nutritional Regulation and Disease Control for Aquaculture, Qinhuangdao, Hebei, ChinaHigher Institution Center of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, University Malaysia Terengganu, Kuala Terengganu, Terengganu, MalaysiaHigher Institution Center of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, University Malaysia Terengganu, Kuala Terengganu, Terengganu, MalaysiaOcean College, Hebei Agricultural University, Qinhuangdao, Hebei, ChinaHebei Key Laboratory of Nutritional Regulation and Disease Control for Aquaculture, Qinhuangdao, Hebei, ChinaOcean College, Hebei Agricultural University, Qinhuangdao, Hebei, ChinaHebei Key Laboratory of Nutritional Regulation and Disease Control for Aquaculture, Qinhuangdao, Hebei, ChinaMandarin fish (Siniperca chuatsi) fed with an artificial diet is progressively gaining popularity, which is important for reducing product prices and resource consumption. However, food is the decisive factor of intestinal microbes, and the profound effects of change in their feeding habit on intestinal microbes of mandarin fish have not been revealed. In the present study, live bait fish and artificial diet were used to feed mandarin fish for 8 weeks to study the effect of different feeding habits on the histology, microbiota structure and dominant bacteria of gut in mandarin fish. The results indicated that feeding with the artificial diet significantly increased the intestinal villi height and muscular thickness in the hindgut of mandarin fish. In addition, the microbiota results showed that there were significant differences of beta diversity of gut microbiota in mandarin fish fed with different diets. At the genus level, feeding artificial diets significantly increased the abundance of Weissella in the mandarin fish gut. Furthermore, two Weissella bacteria were identified and characterized from the midgut contents of mandarin fish fed with artificial diet. Based on 16S rRNA gene sequencing, nine strains were assigned as Weissella confusa (RM125), and one as Weissella cibaria (SJ548). Biochemical analyses based on the VITEK2 method revealed a pattern of metabolic activities against W. confusa RM125 and W. cibaria SJ548, with 13 positive and 29 negative results, respectively. W. confusa RM125 and W. cibaria SJ548 exhibited sensitivities toward a variety of pathogens, including V. harveyi, S. aureus and V. parahaemolyticus, E. coli, A. hydrophila, S. enterica, V. anguillarum, and V. alginolyticus, indicating potential probiotics. Therefore, our results confirmed that the transformation of feeding habit altered the structure, microbial composition and dominant bacteria in gut of mandarin fish, and provided evidence that Weissella might play a crucial role in the digestion and absorption of artificial diet in mandarin fish.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1486501/fullSiniperca chuatsiartificial dietgut histologygut microbiotaWeissella |
| spellingShingle | Jiayu Wang Jiayu Wang Yaotong Hao Yaotong Hao Lihan Zhang Lihan Zhang Xiaowei Gao Xiaowei Gao Yihuan Xu Yihuan Xu Jiangjiang Wang Jiangjiang Wang Fazhan Hanafiah Waiho Khor Yanfeng Sun Yanfeng Sun Chengbin Wu Chengbin Wu Profiling the gut structure and microbiota, and identifying two dominant bacteria belonging to the Weissella genus in mandarin fish (Siniperca chuatsi) fed an artificial diet Frontiers in Microbiology Siniperca chuatsi artificial diet gut histology gut microbiota Weissella |
| title | Profiling the gut structure and microbiota, and identifying two dominant bacteria belonging to the Weissella genus in mandarin fish (Siniperca chuatsi) fed an artificial diet |
| title_full | Profiling the gut structure and microbiota, and identifying two dominant bacteria belonging to the Weissella genus in mandarin fish (Siniperca chuatsi) fed an artificial diet |
| title_fullStr | Profiling the gut structure and microbiota, and identifying two dominant bacteria belonging to the Weissella genus in mandarin fish (Siniperca chuatsi) fed an artificial diet |
| title_full_unstemmed | Profiling the gut structure and microbiota, and identifying two dominant bacteria belonging to the Weissella genus in mandarin fish (Siniperca chuatsi) fed an artificial diet |
| title_short | Profiling the gut structure and microbiota, and identifying two dominant bacteria belonging to the Weissella genus in mandarin fish (Siniperca chuatsi) fed an artificial diet |
| title_sort | profiling the gut structure and microbiota and identifying two dominant bacteria belonging to the weissella genus in mandarin fish siniperca chuatsi fed an artificial diet |
| topic | Siniperca chuatsi artificial diet gut histology gut microbiota Weissella |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1486501/full |
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