Impact of electronic cigarette vaping on the cardiovascular function in young and old rats

Abstract Background While the acute exposure to electronic cigarette (E-cig) vapor has been associated with an increase in blood pressure, the chronic effect of E-cig vapor on blood pressure compared to standard cigarette smoke has not been extensively studied. We determined the effect of E-cig expo...

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Main Authors: Wangde Dai, Jianru Shi, Juan Carreno, Michael T. Kleinman, David A. Herman, Rebecca J. Arechavala, Samantha Renusch, Irene Hasen, Amanda Ting, Robert A. Kloner
Format: Article
Language:English
Published: Nature Portfolio 2024-12-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-024-81398-8
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author Wangde Dai
Jianru Shi
Juan Carreno
Michael T. Kleinman
David A. Herman
Rebecca J. Arechavala
Samantha Renusch
Irene Hasen
Amanda Ting
Robert A. Kloner
author_facet Wangde Dai
Jianru Shi
Juan Carreno
Michael T. Kleinman
David A. Herman
Rebecca J. Arechavala
Samantha Renusch
Irene Hasen
Amanda Ting
Robert A. Kloner
author_sort Wangde Dai
collection DOAJ
description Abstract Background While the acute exposure to electronic cigarette (E-cig) vapor has been associated with an increase in blood pressure, the chronic effect of E-cig vapor on blood pressure compared to standard cigarette smoke has not been extensively studied. We determined the effect of E-cig exposure on blood pressure and other measures of cardiac function in both young and old rats. Methods Young Sprague Dawley rats (6 weeks old, both sexes) were randomly exposed to air (n = 34), E-cig with nicotine (E-cig Nic+; n = 30), E-cig without nicotine (E-cig Nic-; n = 28) or standard cigarette smoke (n = 27). Old Fischer 344 rats (25 months old, both sexes) were randomized into 2 groups: (1) 26 rats in the purified air (negative control) group and (2) 17 rats in the electronic cigarette vapor plus nicotine group (E-cig Nic+). After 12 weeks of exposure, hemodynamics were determined by Millar catheter, echocardiography, and thermodilution catheter, a few days after their last exposure. Results In young rats, cigarette smoke was associated with higher systolic, diastolic and mean blood pressures and peak LV systolic pressure, compared to air or E-cig Nic + or E-cig Nic- groups. Neither fractional shortening nor cardiac output differed among the groups. Absolute value for dp/dt min, a measure of diastolic LV function, was lowest in the E-cig Nic- group. Tau, a measure of LV relaxation was worse in this group as well. In old rats, E-cig vaping did not change heart rate, blood pressure, and cardiac function. However, E-cig Nic + exposure was associated with a greater heart weight/BW and LV weight/BW compared to air exposure in old rats. Conclusions Chronic exposure to E-cig vaping did not cause an increase in blood pressure or heart rate, nor did it change cardiac function compared to air in young rats after 12 weeks of exposure, while standard cigarette smoking was associated with an increase in blood pressure. E-cig vaping was associated with a greater heart weight/BW and LV weight/BW compared to air exposure in old rats, suggested that older animals might be more vulnerable to E-cig stimulus than younger ones.
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spelling doaj-art-11cac6ecdc5a407ebee48fea91e102512024-12-08T12:29:43ZengNature PortfolioScientific Reports2045-23222024-12-0114111010.1038/s41598-024-81398-8Impact of electronic cigarette vaping on the cardiovascular function in young and old ratsWangde Dai0Jianru Shi1Juan Carreno2Michael T. Kleinman3David A. Herman4Rebecca J. Arechavala5Samantha Renusch6Irene Hasen7Amanda Ting8Robert A. Kloner9HMRI Cardiovascular Research Institute, Huntington Medical Research InstitutesHMRI Cardiovascular Research Institute, Huntington Medical Research InstitutesHMRI Cardiovascular Research Institute, Huntington Medical Research InstitutesDepartment of Environmental and Occupational Health, College of Health Sciences, University of CaliforniaDepartment of Environmental and Occupational Health, College of Health Sciences, University of CaliforniaDepartment of Environmental and Occupational Health, College of Health Sciences, University of CaliforniaDepartment of Environmental and Occupational Health, College of Health Sciences, University of CaliforniaDepartment of Environmental and Occupational Health, College of Health Sciences, University of CaliforniaDepartment of Environmental and Occupational Health, College of Health Sciences, University of CaliforniaHMRI Cardiovascular Research Institute, Huntington Medical Research InstitutesAbstract Background While the acute exposure to electronic cigarette (E-cig) vapor has been associated with an increase in blood pressure, the chronic effect of E-cig vapor on blood pressure compared to standard cigarette smoke has not been extensively studied. We determined the effect of E-cig exposure on blood pressure and other measures of cardiac function in both young and old rats. Methods Young Sprague Dawley rats (6 weeks old, both sexes) were randomly exposed to air (n = 34), E-cig with nicotine (E-cig Nic+; n = 30), E-cig without nicotine (E-cig Nic-; n = 28) or standard cigarette smoke (n = 27). Old Fischer 344 rats (25 months old, both sexes) were randomized into 2 groups: (1) 26 rats in the purified air (negative control) group and (2) 17 rats in the electronic cigarette vapor plus nicotine group (E-cig Nic+). After 12 weeks of exposure, hemodynamics were determined by Millar catheter, echocardiography, and thermodilution catheter, a few days after their last exposure. Results In young rats, cigarette smoke was associated with higher systolic, diastolic and mean blood pressures and peak LV systolic pressure, compared to air or E-cig Nic + or E-cig Nic- groups. Neither fractional shortening nor cardiac output differed among the groups. Absolute value for dp/dt min, a measure of diastolic LV function, was lowest in the E-cig Nic- group. Tau, a measure of LV relaxation was worse in this group as well. In old rats, E-cig vaping did not change heart rate, blood pressure, and cardiac function. However, E-cig Nic + exposure was associated with a greater heart weight/BW and LV weight/BW compared to air exposure in old rats. Conclusions Chronic exposure to E-cig vaping did not cause an increase in blood pressure or heart rate, nor did it change cardiac function compared to air in young rats after 12 weeks of exposure, while standard cigarette smoking was associated with an increase in blood pressure. E-cig vaping was associated with a greater heart weight/BW and LV weight/BW compared to air exposure in old rats, suggested that older animals might be more vulnerable to E-cig stimulus than younger ones.https://doi.org/10.1038/s41598-024-81398-8Electronic cigarettesCardiovascular function
spellingShingle Wangde Dai
Jianru Shi
Juan Carreno
Michael T. Kleinman
David A. Herman
Rebecca J. Arechavala
Samantha Renusch
Irene Hasen
Amanda Ting
Robert A. Kloner
Impact of electronic cigarette vaping on the cardiovascular function in young and old rats
Scientific Reports
Electronic cigarettes
Cardiovascular function
title Impact of electronic cigarette vaping on the cardiovascular function in young and old rats
title_full Impact of electronic cigarette vaping on the cardiovascular function in young and old rats
title_fullStr Impact of electronic cigarette vaping on the cardiovascular function in young and old rats
title_full_unstemmed Impact of electronic cigarette vaping on the cardiovascular function in young and old rats
title_short Impact of electronic cigarette vaping on the cardiovascular function in young and old rats
title_sort impact of electronic cigarette vaping on the cardiovascular function in young and old rats
topic Electronic cigarettes
Cardiovascular function
url https://doi.org/10.1038/s41598-024-81398-8
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