Evaluating the Cytotoxic Effects of Ethanol and n-Hexane Extracts from Black Cumin Seeds (Nigella sativa) on B16F10 Mouse Melanoma Cells : A Preliminary Investigation into Vitiligo Treatment
Black cumin (Nigella sativa) is a herbal plant that has been cultivated locally in Indonesia and is traditionally used for various diseases. Thymoquinone, one of the main components, is rich in biological activity. In several countries, topical application of its oil on human skin with vitiligo can...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bogor Agricultural University
2024-11-01
|
| Series: | Hayati Journal of Biosciences |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/54670 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846157861175951360 |
|---|---|
| author | Dian Pratiwi Silmi Mariya Raendi Rayendra Agus Setiyono |
| author_facet | Dian Pratiwi Silmi Mariya Raendi Rayendra Agus Setiyono |
| author_sort | Dian Pratiwi |
| collection | DOAJ |
| description |
Black cumin (Nigella sativa) is a herbal plant that has been cultivated locally in Indonesia and is traditionally used for various diseases. Thymoquinone, one of the main components, is rich in biological activity. In several countries, topical application of its oil on human skin with vitiligo can stimulate skin repigmentation with minimal side effects. This study aims to determine the viability of B16F10 melanoma mouse cells against ethanol and n-hexane extracts of black cumin seeds through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The ethanol extract (EE) yield was 14.306%, and the n-hexane extract (NHE) was 7.442%. Phytochemical screening of EE detected flavonoids, alkaloids, saponins, and steroids, and High-performance liquid chromatography (HPLC) detected 0.040% thymoquinone. The MTT test showed cell viability was >100% from EE at all treatment concentrations, namely 0.75-100 ppm and only 0.75-6.25 ppm for NHE. In conclusion, this study indicates that 96% EE of Nigella sativa is less toxic than NHE on B16F10 mouse melanoma cells and has potential as an alternative treatment for vitiligo, which needs to be proven in further research.
|
| format | Article |
| id | doaj-art-11b1d2c65c494792951144b1061a8745 |
| institution | Kabale University |
| issn | 1978-3019 2086-4094 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj-art-11b1d2c65c494792951144b1061a87452024-11-25T03:41:12ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942024-11-0132110.4308/hjb.32.1.203-211Evaluating the Cytotoxic Effects of Ethanol and n-Hexane Extracts from Black Cumin Seeds (Nigella sativa) on B16F10 Mouse Melanoma Cells : A Preliminary Investigation into Vitiligo TreatmentDian Pratiwi0Silmi Mariya1Raendi Rayendra2Agus Setiyono3Doctoral Program, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor 16680, IndonesiaPrimate Research Center Animal Studies, IPB University, Bogor 16129, IndonesiaFaculty of Medicine, State Islamic University Syarif Hidayatullah, Jakarta, IndonesiaDivision of Pathology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor 16680, Indonesia Black cumin (Nigella sativa) is a herbal plant that has been cultivated locally in Indonesia and is traditionally used for various diseases. Thymoquinone, one of the main components, is rich in biological activity. In several countries, topical application of its oil on human skin with vitiligo can stimulate skin repigmentation with minimal side effects. This study aims to determine the viability of B16F10 melanoma mouse cells against ethanol and n-hexane extracts of black cumin seeds through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The ethanol extract (EE) yield was 14.306%, and the n-hexane extract (NHE) was 7.442%. Phytochemical screening of EE detected flavonoids, alkaloids, saponins, and steroids, and High-performance liquid chromatography (HPLC) detected 0.040% thymoquinone. The MTT test showed cell viability was >100% from EE at all treatment concentrations, namely 0.75-100 ppm and only 0.75-6.25 ppm for NHE. In conclusion, this study indicates that 96% EE of Nigella sativa is less toxic than NHE on B16F10 mouse melanoma cells and has potential as an alternative treatment for vitiligo, which needs to be proven in further research. https://journal.ipb.ac.id/index.php/hayati/article/view/54670 |
| spellingShingle | Dian Pratiwi Silmi Mariya Raendi Rayendra Agus Setiyono Evaluating the Cytotoxic Effects of Ethanol and n-Hexane Extracts from Black Cumin Seeds (Nigella sativa) on B16F10 Mouse Melanoma Cells : A Preliminary Investigation into Vitiligo Treatment Hayati Journal of Biosciences |
| title | Evaluating the Cytotoxic Effects of Ethanol and n-Hexane Extracts from Black Cumin Seeds (Nigella sativa) on B16F10 Mouse Melanoma Cells : A Preliminary Investigation into Vitiligo Treatment |
| title_full | Evaluating the Cytotoxic Effects of Ethanol and n-Hexane Extracts from Black Cumin Seeds (Nigella sativa) on B16F10 Mouse Melanoma Cells : A Preliminary Investigation into Vitiligo Treatment |
| title_fullStr | Evaluating the Cytotoxic Effects of Ethanol and n-Hexane Extracts from Black Cumin Seeds (Nigella sativa) on B16F10 Mouse Melanoma Cells : A Preliminary Investigation into Vitiligo Treatment |
| title_full_unstemmed | Evaluating the Cytotoxic Effects of Ethanol and n-Hexane Extracts from Black Cumin Seeds (Nigella sativa) on B16F10 Mouse Melanoma Cells : A Preliminary Investigation into Vitiligo Treatment |
| title_short | Evaluating the Cytotoxic Effects of Ethanol and n-Hexane Extracts from Black Cumin Seeds (Nigella sativa) on B16F10 Mouse Melanoma Cells : A Preliminary Investigation into Vitiligo Treatment |
| title_sort | evaluating the cytotoxic effects of ethanol and n hexane extracts from black cumin seeds nigella sativa on b16f10 mouse melanoma cells a preliminary investigation into vitiligo treatment |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/54670 |
| work_keys_str_mv | AT dianpratiwi evaluatingthecytotoxiceffectsofethanolandnhexaneextractsfromblackcuminseedsnigellasativaonb16f10mousemelanomacellsapreliminaryinvestigationintovitiligotreatment AT silmimariya evaluatingthecytotoxiceffectsofethanolandnhexaneextractsfromblackcuminseedsnigellasativaonb16f10mousemelanomacellsapreliminaryinvestigationintovitiligotreatment AT raendirayendra evaluatingthecytotoxiceffectsofethanolandnhexaneextractsfromblackcuminseedsnigellasativaonb16f10mousemelanomacellsapreliminaryinvestigationintovitiligotreatment AT agussetiyono evaluatingthecytotoxiceffectsofethanolandnhexaneextractsfromblackcuminseedsnigellasativaonb16f10mousemelanomacellsapreliminaryinvestigationintovitiligotreatment |