Multiplex basophil activation tests for allergy diagnosis: present and future applications
The basophil activation test (BAT) has become a major cellular in vitro test for evaluating the allergenic activity of specific IgEs. The impact of the BAT is due to the ability of blood basophil granulocytes to present IgE on the high-affinity FcεRI receptor and to mirror the mast cell response tha...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Allergy |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/falgy.2024.1515843/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841540091199094784 |
|---|---|
| author | Ana Koren Peter Korosec Peter Korosec |
| author_facet | Ana Koren Peter Korosec Peter Korosec |
| author_sort | Ana Koren |
| collection | DOAJ |
| description | The basophil activation test (BAT) has become a major cellular in vitro test for evaluating the allergenic activity of specific IgEs. The impact of the BAT is due to the ability of blood basophil granulocytes to present IgE on the high-affinity FcεRI receptor and to mirror the mast cell response that elicits an acute allergic reaction. The BAT proved to be able to identify allergic patients at risk of reacting to a low dose of the allergen and/or developing life-threatening reactions and thus can significantly improve the current management of allergic patients. However, to improve the diagnostic utility for identifying the allergenic activity of different genuinely sensitizing allergens and lower the procedure and labour requirements, developing a multiplex BAT approach incorporating multiple allergen components would be highly anticipated. Recently, the novel multiplex BAT was described utilizing two major innovative steps. The first step was the fluorescent labeling of allergens. The second step was applying fluorescently labeled allergens in flow cytometry assessment to analyze the activation of basophil subpopulations gated according to the binding of different allergens or to evaluate the fluorescence intensity of multiple allergens on the surface of basophils. These novel cellular multiplex approaches will advance our understanding of IgE-mediated responses. Integration of multiplex BAT, in addition to multiplex IgE assays into practice, will personalize the measurement of allergenic IgE activity and can help estimate the likelihood of clinical relevance and risks for multiple allergens when testing individual allergic patients. |
| format | Article |
| id | doaj-art-11ab3d2efc5a487fa1bdbd260774c97c |
| institution | Kabale University |
| issn | 2673-6101 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Allergy |
| spelling | doaj-art-11ab3d2efc5a487fa1bdbd260774c97c2025-01-14T06:10:37ZengFrontiers Media S.A.Frontiers in Allergy2673-61012025-01-01510.3389/falgy.2024.15158431515843Multiplex basophil activation tests for allergy diagnosis: present and future applicationsAna Koren0Peter Korosec1Peter Korosec2Laboratory for Clinical Immunology and Molecular Genetics, University Clinic of Respiratory and Allergic Diseases Golnik, Golnik, SloveniaLaboratory for Clinical Immunology and Molecular Genetics, University Clinic of Respiratory and Allergic Diseases Golnik, Golnik, SloveniaFaculty of Pharmacy, University of Ljubljana, Ljubljana, SloveniaThe basophil activation test (BAT) has become a major cellular in vitro test for evaluating the allergenic activity of specific IgEs. The impact of the BAT is due to the ability of blood basophil granulocytes to present IgE on the high-affinity FcεRI receptor and to mirror the mast cell response that elicits an acute allergic reaction. The BAT proved to be able to identify allergic patients at risk of reacting to a low dose of the allergen and/or developing life-threatening reactions and thus can significantly improve the current management of allergic patients. However, to improve the diagnostic utility for identifying the allergenic activity of different genuinely sensitizing allergens and lower the procedure and labour requirements, developing a multiplex BAT approach incorporating multiple allergen components would be highly anticipated. Recently, the novel multiplex BAT was described utilizing two major innovative steps. The first step was the fluorescent labeling of allergens. The second step was applying fluorescently labeled allergens in flow cytometry assessment to analyze the activation of basophil subpopulations gated according to the binding of different allergens or to evaluate the fluorescence intensity of multiple allergens on the surface of basophils. These novel cellular multiplex approaches will advance our understanding of IgE-mediated responses. Integration of multiplex BAT, in addition to multiplex IgE assays into practice, will personalize the measurement of allergenic IgE activity and can help estimate the likelihood of clinical relevance and risks for multiple allergens when testing individual allergic patients.https://www.frontiersin.org/articles/10.3389/falgy.2024.1515843/fullallergydiagnosisbasophil activation testCD63multiplex analysis |
| spellingShingle | Ana Koren Peter Korosec Peter Korosec Multiplex basophil activation tests for allergy diagnosis: present and future applications Frontiers in Allergy allergy diagnosis basophil activation test CD63 multiplex analysis |
| title | Multiplex basophil activation tests for allergy diagnosis: present and future applications |
| title_full | Multiplex basophil activation tests for allergy diagnosis: present and future applications |
| title_fullStr | Multiplex basophil activation tests for allergy diagnosis: present and future applications |
| title_full_unstemmed | Multiplex basophil activation tests for allergy diagnosis: present and future applications |
| title_short | Multiplex basophil activation tests for allergy diagnosis: present and future applications |
| title_sort | multiplex basophil activation tests for allergy diagnosis present and future applications |
| topic | allergy diagnosis basophil activation test CD63 multiplex analysis |
| url | https://www.frontiersin.org/articles/10.3389/falgy.2024.1515843/full |
| work_keys_str_mv | AT anakoren multiplexbasophilactivationtestsforallergydiagnosispresentandfutureapplications AT peterkorosec multiplexbasophilactivationtestsforallergydiagnosispresentandfutureapplications AT peterkorosec multiplexbasophilactivationtestsforallergydiagnosispresentandfutureapplications |