Computer identification of Notch3 in the neurogenic progenitor cells of mammalian early optic vesicles
Abstract Objective The developing mammalian retina initially contains undifferentiated cells, providing a model for investigating the mechanisms of differentiation. Notch signaling, mediated by four Notch receptors (Notch 1–4) in mammals, has been studied in the differentiation of neural progenitor...
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BMC
2025-08-01
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| Series: | BMC Research Notes |
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| Online Access: | https://doi.org/10.1186/s13104-025-07440-8 |
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| author | Yoshitoku Watabe Sakurako Kobayashi Satoru Takahashi Masaharu Yoshihara |
| author_facet | Yoshitoku Watabe Sakurako Kobayashi Satoru Takahashi Masaharu Yoshihara |
| author_sort | Yoshitoku Watabe |
| collection | DOAJ |
| description | Abstract Objective The developing mammalian retina initially contains undifferentiated cells, providing a model for investigating the mechanisms of differentiation. Notch signaling, mediated by four Notch receptors (Notch 1–4) in mammals, has been studied in the differentiation of neural progenitor cells. Among the four Notch receptors, the frequency, rather than the peak level, of Notch1-mediated signaling has been suggested to promote the activation of neural progenitor cells. In contrast to Notch1, the involvement of Notch3 in this process is poorly documented, although Notch3 is known for its role in vascular integrity. Results By re-analyzing publicly available single-cell RNA-seq data from one mouse retinal dataset, two human retinal organoid datasets and two human embryonic retinal datasets, we found that, along with Notch1, Notch3 is expressed in neural progenitor cells in the retina. In addition, the results of the co-expression profile analyses varied among the datasets, leaving uncertainty regarding the regulatory mechanisms of Notch1 and Notch3. Our findings shed light on Notch3 in neurogenic progenitor cells of the developing mammalian retina. Since Notch3 has been suggested to cause ligand-independent signaling, Notch3 expression might antagonize Notch1-mediated signaling oscillations, maintaining the quiescent state of neurogenic progenitor cells. |
| format | Article |
| id | doaj-art-10e828f78bb149db9e1c6d38f1203f23 |
| institution | Kabale University |
| issn | 1756-0500 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | BMC |
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| series | BMC Research Notes |
| spelling | doaj-art-10e828f78bb149db9e1c6d38f1203f232025-08-24T11:06:10ZengBMCBMC Research Notes1756-05002025-08-011811810.1186/s13104-025-07440-8Computer identification of Notch3 in the neurogenic progenitor cells of mammalian early optic vesiclesYoshitoku Watabe0Sakurako Kobayashi1Satoru Takahashi2Masaharu Yoshihara3College of Medicine, School of Medicine and Health Sciences, University of TsukubaCollege of Medicine, School of Medicine and Health Sciences, University of TsukubaDepartment of Anatomy and Embryology, Institute of Medicine, University of TsukubaDepartment of Anatomy and Embryology, Institute of Medicine, University of TsukubaAbstract Objective The developing mammalian retina initially contains undifferentiated cells, providing a model for investigating the mechanisms of differentiation. Notch signaling, mediated by four Notch receptors (Notch 1–4) in mammals, has been studied in the differentiation of neural progenitor cells. Among the four Notch receptors, the frequency, rather than the peak level, of Notch1-mediated signaling has been suggested to promote the activation of neural progenitor cells. In contrast to Notch1, the involvement of Notch3 in this process is poorly documented, although Notch3 is known for its role in vascular integrity. Results By re-analyzing publicly available single-cell RNA-seq data from one mouse retinal dataset, two human retinal organoid datasets and two human embryonic retinal datasets, we found that, along with Notch1, Notch3 is expressed in neural progenitor cells in the retina. In addition, the results of the co-expression profile analyses varied among the datasets, leaving uncertainty regarding the regulatory mechanisms of Notch1 and Notch3. Our findings shed light on Notch3 in neurogenic progenitor cells of the developing mammalian retina. Since Notch3 has been suggested to cause ligand-independent signaling, Notch3 expression might antagonize Notch1-mediated signaling oscillations, maintaining the quiescent state of neurogenic progenitor cells.https://doi.org/10.1186/s13104-025-07440-8Hes1Notch signalingOscillationQuiescenceRetinaRetinal progenitor cell |
| spellingShingle | Yoshitoku Watabe Sakurako Kobayashi Satoru Takahashi Masaharu Yoshihara Computer identification of Notch3 in the neurogenic progenitor cells of mammalian early optic vesicles BMC Research Notes Hes1 Notch signaling Oscillation Quiescence Retina Retinal progenitor cell |
| title | Computer identification of Notch3 in the neurogenic progenitor cells of mammalian early optic vesicles |
| title_full | Computer identification of Notch3 in the neurogenic progenitor cells of mammalian early optic vesicles |
| title_fullStr | Computer identification of Notch3 in the neurogenic progenitor cells of mammalian early optic vesicles |
| title_full_unstemmed | Computer identification of Notch3 in the neurogenic progenitor cells of mammalian early optic vesicles |
| title_short | Computer identification of Notch3 in the neurogenic progenitor cells of mammalian early optic vesicles |
| title_sort | computer identification of notch3 in the neurogenic progenitor cells of mammalian early optic vesicles |
| topic | Hes1 Notch signaling Oscillation Quiescence Retina Retinal progenitor cell |
| url | https://doi.org/10.1186/s13104-025-07440-8 |
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