Enteral nutrition ameliorates the symptoms of Crohn's disease in mice via activating special pro-resolving mediators through innate lymphoid cells
Crohn's disease activates the inflammatory reactions to induce intestinal disorders. Enteral nutrition (EN) could exert general immunomodulatory effects. Cecal ligation and perforation (CLP) surgery was utilized to establish Crohn's disease mice models. Survival analysis, hematoxylin-eosin...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2021-10-01
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| Series: | Innate Immunity |
| Online Access: | https://doi.org/10.1177/17534259211057038 |
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| _version_ | 1846161941779709952 |
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| author | Di Zhao Bo Yang Chen Ye Shaoyi Zhang Xiaoqiong Lv Qiyi Chen |
| author_facet | Di Zhao Bo Yang Chen Ye Shaoyi Zhang Xiaoqiong Lv Qiyi Chen |
| author_sort | Di Zhao |
| collection | DOAJ |
| description | Crohn's disease activates the inflammatory reactions to induce intestinal disorders. Enteral nutrition (EN) could exert general immunomodulatory effects. Cecal ligation and perforation (CLP) surgery was utilized to establish Crohn's disease mice models. Survival analysis, hematoxylin-eosin staining, flow cytometry, ELISA, Western blot and liquid chromatography-tandem MS were applied. Baicalein was added to inhibit lipoxygenases. The survival rate was restored and inflammatory injury, exudate neutrophils in peritoneal lavage and serum levels of IL-6 and TNF-α were ameliorated by EN treatment as compared with CLP treatment. EN also increased ILC-3 content, 5/15-LOX level and RvD1-RvD5 in peritoneal lavage. Baicalein reversed all the detected effects of EN except ILC-3 content. EN could activate special pro-resolving mediators (SPMs) through ILCs to mitigate injuries of Crohn's disease. |
| format | Article |
| id | doaj-art-10c70fa7c5764dbcbb0eab950b1a82ab |
| institution | Kabale University |
| issn | 1753-4259 1753-4267 |
| language | English |
| publishDate | 2021-10-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Innate Immunity |
| spelling | doaj-art-10c70fa7c5764dbcbb0eab950b1a82ab2024-11-20T20:03:20ZengSAGE PublishingInnate Immunity1753-42591753-42672021-10-012710.1177/17534259211057038Enteral nutrition ameliorates the symptoms of Crohn's disease in mice via activating special pro-resolving mediators through innate lymphoid cellsDi Zhao0Bo Yang1Chen Ye2Shaoyi Zhang3Xiaoqiong Lv4Qiyi Chen5Clinical Research Center for Digestive Diseases, Tongji University, Shanghai 200072, ChinaClinical Research Center for Digestive Diseases, Tongji University, Shanghai 200072, ChinaClinical Research Center for Digestive Diseases, Tongji University, Shanghai 200072, ChinaClinical Research Center for Digestive Diseases, Tongji University, Shanghai 200072, ChinaClinical Research Center for Digestive Diseases, Tongji University, Shanghai 200072, ChinaClinical Research Center for Digestive Diseases, Tongji University, Shanghai 200072, ChinaCrohn's disease activates the inflammatory reactions to induce intestinal disorders. Enteral nutrition (EN) could exert general immunomodulatory effects. Cecal ligation and perforation (CLP) surgery was utilized to establish Crohn's disease mice models. Survival analysis, hematoxylin-eosin staining, flow cytometry, ELISA, Western blot and liquid chromatography-tandem MS were applied. Baicalein was added to inhibit lipoxygenases. The survival rate was restored and inflammatory injury, exudate neutrophils in peritoneal lavage and serum levels of IL-6 and TNF-α were ameliorated by EN treatment as compared with CLP treatment. EN also increased ILC-3 content, 5/15-LOX level and RvD1-RvD5 in peritoneal lavage. Baicalein reversed all the detected effects of EN except ILC-3 content. EN could activate special pro-resolving mediators (SPMs) through ILCs to mitigate injuries of Crohn's disease.https://doi.org/10.1177/17534259211057038 |
| spellingShingle | Di Zhao Bo Yang Chen Ye Shaoyi Zhang Xiaoqiong Lv Qiyi Chen Enteral nutrition ameliorates the symptoms of Crohn's disease in mice via activating special pro-resolving mediators through innate lymphoid cells Innate Immunity |
| title | Enteral nutrition ameliorates the symptoms of Crohn's disease in mice via activating special pro-resolving mediators through innate lymphoid cells |
| title_full | Enteral nutrition ameliorates the symptoms of Crohn's disease in mice via activating special pro-resolving mediators through innate lymphoid cells |
| title_fullStr | Enteral nutrition ameliorates the symptoms of Crohn's disease in mice via activating special pro-resolving mediators through innate lymphoid cells |
| title_full_unstemmed | Enteral nutrition ameliorates the symptoms of Crohn's disease in mice via activating special pro-resolving mediators through innate lymphoid cells |
| title_short | Enteral nutrition ameliorates the symptoms of Crohn's disease in mice via activating special pro-resolving mediators through innate lymphoid cells |
| title_sort | enteral nutrition ameliorates the symptoms of crohn s disease in mice via activating special pro resolving mediators through innate lymphoid cells |
| url | https://doi.org/10.1177/17534259211057038 |
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