Dynamic metabolomic changes in Pochonia chlamydosporia’s parasitism of Parascaris equorum eggs
Pochonia chlamydosporia, a nematophagous fungus, holds great promise as a biological control agent against animal − parasitic nematodes. However, the molecular and cellular mechanisms of its infection process remain largely unclear. In this study, metabolomics was utilized to investigate the dynamic...
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| Language: | English |
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Elsevier
2025-09-01
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| Series: | Biological Control |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1049964425001598 |
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| author | Luyao Hao Fengmiao Zhao Hongyou Liu Chengyu Ma Yuan Ma Zhengyi Li Wei Wei Rui Wang |
| author_facet | Luyao Hao Fengmiao Zhao Hongyou Liu Chengyu Ma Yuan Ma Zhengyi Li Wei Wei Rui Wang |
| author_sort | Luyao Hao |
| collection | DOAJ |
| description | Pochonia chlamydosporia, a nematophagous fungus, holds great promise as a biological control agent against animal − parasitic nematodes. However, the molecular and cellular mechanisms of its infection process remain largely unclear. In this study, metabolomics was utilized to investigate the dynamic changes in the exometabolome during the infection of P. chlamydosporia on Parascaris equorum eggs. Three crucial infection stages were selected: early (A1), middle (B1), and late (C1), with control groups of P. chlamydosporia hyphae cultured without eggs (A, B, C). Metabolite extraction was carried out, followed by Liquid Chromatography-Tandem Mass Spectrometry (LC − MS/MS) analysis to identify differentially accumulated metabolites. LC-MS/MS analysis identified 1,185 fungal-derived metabolites, with key players including Ascochalasin (membrane disruptor), Piperine (signal transducer), and 6-methoxygossypol (egg development inhibitor). These metabolites orchestrated dynamic processes: organic acids fueled TCA cycle energy supply during mid-stage infection, sphingolipids mediated membrane fusion in late stages, and alkaloids disrupted host membrane permeability. Pathway analysis revealed stage-specific hubs: alanine-aspartate metabolism dominated early infection for nitrogen acquisition, cAMP signaling peaked in mid-stage to hijack host pathways, and secondary bile acid biosynthesis surged late-stage to degrade eggshells. These findings clarify that P. chlamydosporia coordinates a metabolic cascade—from energy reprogramming to host defense evasion—to complete parasitism, providing novel targets for biocontrol agent development. |
| format | Article |
| id | doaj-art-10c402e9ed3945dfb749263f9ad1b8d5 |
| institution | Kabale University |
| issn | 1049-9644 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biological Control |
| spelling | doaj-art-10c402e9ed3945dfb749263f9ad1b8d52025-08-20T04:01:48ZengElsevierBiological Control1049-96442025-09-0120810584910.1016/j.biocontrol.2025.105849Dynamic metabolomic changes in Pochonia chlamydosporia’s parasitism of Parascaris equorum eggsLuyao Hao0Fengmiao Zhao1Hongyou Liu2Chengyu Ma3Yuan Ma4Zhengyi Li5Wei Wei6Rui Wang7College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, PR China; Key Laboratory of Clinical Diagnosis and Treatment of Animal Diseases, Ministry of Agriculture, National Animal Medicine Experimental Teaching Center, PR ChinaCollege of Grassland Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, PR ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, PR China; Key Laboratory of Clinical Diagnosis and Treatment of Animal Diseases, Ministry of Agriculture, National Animal Medicine Experimental Teaching Center, PR ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, PR China; Key Laboratory of Clinical Diagnosis and Treatment of Animal Diseases, Ministry of Agriculture, National Animal Medicine Experimental Teaching Center, PR ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, PR China; Key Laboratory of Clinical Diagnosis and Treatment of Animal Diseases, Ministry of Agriculture, National Animal Medicine Experimental Teaching Center, PR ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, PR China; Key Laboratory of Clinical Diagnosis and Treatment of Animal Diseases, Ministry of Agriculture, National Animal Medicine Experimental Teaching Center, PR ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, PR China; Key Laboratory of Clinical Diagnosis and Treatment of Animal Diseases, Ministry of Agriculture, National Animal Medicine Experimental Teaching Center, PR ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, PR China; Key Laboratory of Clinical Diagnosis and Treatment of Animal Diseases, Ministry of Agriculture, National Animal Medicine Experimental Teaching Center, PR China; Corresponding author at: College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, PR China.Pochonia chlamydosporia, a nematophagous fungus, holds great promise as a biological control agent against animal − parasitic nematodes. However, the molecular and cellular mechanisms of its infection process remain largely unclear. In this study, metabolomics was utilized to investigate the dynamic changes in the exometabolome during the infection of P. chlamydosporia on Parascaris equorum eggs. Three crucial infection stages were selected: early (A1), middle (B1), and late (C1), with control groups of P. chlamydosporia hyphae cultured without eggs (A, B, C). Metabolite extraction was carried out, followed by Liquid Chromatography-Tandem Mass Spectrometry (LC − MS/MS) analysis to identify differentially accumulated metabolites. LC-MS/MS analysis identified 1,185 fungal-derived metabolites, with key players including Ascochalasin (membrane disruptor), Piperine (signal transducer), and 6-methoxygossypol (egg development inhibitor). These metabolites orchestrated dynamic processes: organic acids fueled TCA cycle energy supply during mid-stage infection, sphingolipids mediated membrane fusion in late stages, and alkaloids disrupted host membrane permeability. Pathway analysis revealed stage-specific hubs: alanine-aspartate metabolism dominated early infection for nitrogen acquisition, cAMP signaling peaked in mid-stage to hijack host pathways, and secondary bile acid biosynthesis surged late-stage to degrade eggshells. These findings clarify that P. chlamydosporia coordinates a metabolic cascade—from energy reprogramming to host defense evasion—to complete parasitism, providing novel targets for biocontrol agent development.http://www.sciencedirect.com/science/article/pii/S1049964425001598Helminth eggs-parasitic fungiBiological controlDifferential metabolite expressionLC-MS/MS |
| spellingShingle | Luyao Hao Fengmiao Zhao Hongyou Liu Chengyu Ma Yuan Ma Zhengyi Li Wei Wei Rui Wang Dynamic metabolomic changes in Pochonia chlamydosporia’s parasitism of Parascaris equorum eggs Biological Control Helminth eggs-parasitic fungi Biological control Differential metabolite expression LC-MS/MS |
| title | Dynamic metabolomic changes in Pochonia chlamydosporia’s parasitism of Parascaris equorum eggs |
| title_full | Dynamic metabolomic changes in Pochonia chlamydosporia’s parasitism of Parascaris equorum eggs |
| title_fullStr | Dynamic metabolomic changes in Pochonia chlamydosporia’s parasitism of Parascaris equorum eggs |
| title_full_unstemmed | Dynamic metabolomic changes in Pochonia chlamydosporia’s parasitism of Parascaris equorum eggs |
| title_short | Dynamic metabolomic changes in Pochonia chlamydosporia’s parasitism of Parascaris equorum eggs |
| title_sort | dynamic metabolomic changes in pochonia chlamydosporia s parasitism of parascaris equorum eggs |
| topic | Helminth eggs-parasitic fungi Biological control Differential metabolite expression LC-MS/MS |
| url | http://www.sciencedirect.com/science/article/pii/S1049964425001598 |
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