Palmitoleic Acid (N-7) Attenuates the Immunometabolic Disturbances Caused by a High-Fat Diet Independently of PPARα
Palmitoleic acid (PMA) has anti-inflammatory and antidiabetic activities. Here we tested whether these effects of PMA on glucose homeostasis and liver inflammation, in mice fed with high-fat diet (HFD), are PPAR-α dependent. C57BL6 wild-type (WT) and PPAR-α-knockout (KO) mice fed with a standard die...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/582197 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849304499997376512 |
|---|---|
| author | Camila O. Souza Alexandre A. S. Teixeira Edson A. Lima Helena A. P. Batatinha Lara M. Gomes Milena Carvalho-Silva Isabella T. Mota Emilio L. Streck Sandro M. Hirabara José C. Rosa Neto |
| author_facet | Camila O. Souza Alexandre A. S. Teixeira Edson A. Lima Helena A. P. Batatinha Lara M. Gomes Milena Carvalho-Silva Isabella T. Mota Emilio L. Streck Sandro M. Hirabara José C. Rosa Neto |
| author_sort | Camila O. Souza |
| collection | DOAJ |
| description | Palmitoleic acid (PMA) has anti-inflammatory and antidiabetic activities. Here we tested whether these effects of PMA on glucose homeostasis and liver inflammation, in mice fed with high-fat diet (HFD), are PPAR-α dependent. C57BL6 wild-type (WT) and PPAR-α-knockout (KO) mice fed with a standard diet (SD) or HFD for 12 weeks were treated after the 10th week with oleic acid (OLA, 300 mg/kg of b.w.) or PMA 300 mg/kg of b.w. Steatosis induced by HFD was associated with liver inflammation only in the KO mice, as shown by the increased hepatic levels of IL1-beta, IL-12, and TNF-α; however, the HFD increased the expression of TLR4 and decreased the expression of IL1-Ra in both genotypes. Treatment with palmitoleate markedly attenuated the insulin resistance induced by the HFD, increased glucose uptake and incorporation into muscle in vitro, reduced the serum levels of AST in WT mice, decreased the hepatic levels of IL1-beta and IL-12 in KO mice, reduced the expression of TLR-4 and increased the expression of IL-1Ra in WT mice, and reduced the phosphorylation of NF 𝜅B (p65) in the livers of KO mice. We conclude that palmitoleate attenuates diet-induced insulin resistance, liver inflammation, and damage through mechanisms that do not depend on PPAR-α. |
| format | Article |
| id | doaj-art-1087d1915e7b45c4968cb2d11db3d7f3 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-1087d1915e7b45c4968cb2d11db3d7f32025-08-20T03:55:44ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/582197582197Palmitoleic Acid (N-7) Attenuates the Immunometabolic Disturbances Caused by a High-Fat Diet Independently of PPARαCamila O. Souza0Alexandre A. S. Teixeira1Edson A. Lima2Helena A. P. Batatinha3Lara M. Gomes4Milena Carvalho-Silva5Isabella T. Mota6Emilio L. Streck7Sandro M. Hirabara8José C. Rosa Neto9Department of Cell and Developmental Biology, University of São Paulo, Avenida Lineu Prestes 1524, 05508-000 São Paulo, SP, BrazilDepartment of Cell and Developmental Biology, University of São Paulo, Avenida Lineu Prestes 1524, 05508-000 São Paulo, SP, BrazilDepartment of Cell and Developmental Biology, University of São Paulo, Avenida Lineu Prestes 1524, 05508-000 São Paulo, SP, BrazilDepartment of Cell and Developmental Biology, University of São Paulo, Avenida Lineu Prestes 1524, 05508-000 São Paulo, SP, BrazilBioenergetic Laboratory, Extremo Sul Catarinense University, 88806-000 Criciuma, SC, BrazilBioenergetic Laboratory, Extremo Sul Catarinense University, 88806-000 Criciuma, SC, BrazilBioenergetic Laboratory, Extremo Sul Catarinense University, 88806-000 Criciuma, SC, BrazilBioenergetic Laboratory, Extremo Sul Catarinense University, 88806-000 Criciuma, SC, BrazilInstitute of Physical Activity Sciences and Sports, Cruzeiro do Sul University, 01506-000 São Paulo, SP, BrazilDepartment of Cell and Developmental Biology, University of São Paulo, Avenida Lineu Prestes 1524, 05508-000 São Paulo, SP, BrazilPalmitoleic acid (PMA) has anti-inflammatory and antidiabetic activities. Here we tested whether these effects of PMA on glucose homeostasis and liver inflammation, in mice fed with high-fat diet (HFD), are PPAR-α dependent. C57BL6 wild-type (WT) and PPAR-α-knockout (KO) mice fed with a standard diet (SD) or HFD for 12 weeks were treated after the 10th week with oleic acid (OLA, 300 mg/kg of b.w.) or PMA 300 mg/kg of b.w. Steatosis induced by HFD was associated with liver inflammation only in the KO mice, as shown by the increased hepatic levels of IL1-beta, IL-12, and TNF-α; however, the HFD increased the expression of TLR4 and decreased the expression of IL1-Ra in both genotypes. Treatment with palmitoleate markedly attenuated the insulin resistance induced by the HFD, increased glucose uptake and incorporation into muscle in vitro, reduced the serum levels of AST in WT mice, decreased the hepatic levels of IL1-beta and IL-12 in KO mice, reduced the expression of TLR-4 and increased the expression of IL-1Ra in WT mice, and reduced the phosphorylation of NF 𝜅B (p65) in the livers of KO mice. We conclude that palmitoleate attenuates diet-induced insulin resistance, liver inflammation, and damage through mechanisms that do not depend on PPAR-α.http://dx.doi.org/10.1155/2014/582197 |
| spellingShingle | Camila O. Souza Alexandre A. S. Teixeira Edson A. Lima Helena A. P. Batatinha Lara M. Gomes Milena Carvalho-Silva Isabella T. Mota Emilio L. Streck Sandro M. Hirabara José C. Rosa Neto Palmitoleic Acid (N-7) Attenuates the Immunometabolic Disturbances Caused by a High-Fat Diet Independently of PPARα Mediators of Inflammation |
| title | Palmitoleic Acid (N-7) Attenuates the Immunometabolic Disturbances Caused by a High-Fat Diet Independently of PPARα |
| title_full | Palmitoleic Acid (N-7) Attenuates the Immunometabolic Disturbances Caused by a High-Fat Diet Independently of PPARα |
| title_fullStr | Palmitoleic Acid (N-7) Attenuates the Immunometabolic Disturbances Caused by a High-Fat Diet Independently of PPARα |
| title_full_unstemmed | Palmitoleic Acid (N-7) Attenuates the Immunometabolic Disturbances Caused by a High-Fat Diet Independently of PPARα |
| title_short | Palmitoleic Acid (N-7) Attenuates the Immunometabolic Disturbances Caused by a High-Fat Diet Independently of PPARα |
| title_sort | palmitoleic acid n 7 attenuates the immunometabolic disturbances caused by a high fat diet independently of pparα |
| url | http://dx.doi.org/10.1155/2014/582197 |
| work_keys_str_mv | AT camilaosouza palmitoleicacidn7attenuatestheimmunometabolicdisturbancescausedbyahighfatdietindependentlyofppara AT alexandreasteixeira palmitoleicacidn7attenuatestheimmunometabolicdisturbancescausedbyahighfatdietindependentlyofppara AT edsonalima palmitoleicacidn7attenuatestheimmunometabolicdisturbancescausedbyahighfatdietindependentlyofppara AT helenaapbatatinha palmitoleicacidn7attenuatestheimmunometabolicdisturbancescausedbyahighfatdietindependentlyofppara AT laramgomes palmitoleicacidn7attenuatestheimmunometabolicdisturbancescausedbyahighfatdietindependentlyofppara AT milenacarvalhosilva palmitoleicacidn7attenuatestheimmunometabolicdisturbancescausedbyahighfatdietindependentlyofppara AT isabellatmota palmitoleicacidn7attenuatestheimmunometabolicdisturbancescausedbyahighfatdietindependentlyofppara AT emiliolstreck palmitoleicacidn7attenuatestheimmunometabolicdisturbancescausedbyahighfatdietindependentlyofppara AT sandromhirabara palmitoleicacidn7attenuatestheimmunometabolicdisturbancescausedbyahighfatdietindependentlyofppara AT josecrosaneto palmitoleicacidn7attenuatestheimmunometabolicdisturbancescausedbyahighfatdietindependentlyofppara |