Rapid detection of spermine by using a cost-effective carbon nanostructured aptasensor
Spermine is a polyamine that participates in ion channel regulation and cell proliferation. It is considered a biomarker for pancreatic cancer. In this study, a carbon nanostructure-based aptasensor has been developed to rapidly detect spermine in solution by relying on Förster resonance energy tran...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2024-12-01
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| Series: | Nano TransMed |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2790676024000086 |
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| _version_ | 1846117461334687744 |
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| author | Howyn Tang Hossein Pouri Chao Lu Jin Zhang |
| author_facet | Howyn Tang Hossein Pouri Chao Lu Jin Zhang |
| author_sort | Howyn Tang |
| collection | DOAJ |
| description | Spermine is a polyamine that participates in ion channel regulation and cell proliferation. It is considered a biomarker for pancreatic cancer. In this study, a carbon nanostructure-based aptasensor has been developed to rapidly detect spermine in solution by relying on Förster resonance energy transfer (FRET). The FRET donor, carbon quantum dots (CDs), are bioconjugated to aptamers specific for spermine. CDs have an average diameter of 5.0 ± 2.5 nm and have the maximum fluorescence intensity emitting at 510 nm when excited at 400 nm. Due to the π-π* interaction between DNA-based aptamer and GO, a FRET quencher, CDs bioconjugated with aptamer (CDs-aptamer) can directly attach on the surface of GO, resulting in significant FRET quenching. In the presence of spermine, GO and CDs-Aptamer separate, and the fluorescence intensity of CDs is restored. The restored fluorescence intensity of CD-aptamer can be observed when the concentration of spermine increases from 0.1 to 250 nM. The sensing system in this study could be a cost-effective, user-friendly method to quickly detect spermine, a biomarker for pancreatic cancer. |
| format | Article |
| id | doaj-art-106cfe69852a4ac3b0c71fdca6c68e7c |
| institution | Kabale University |
| issn | 2790-6760 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Nano TransMed |
| spelling | doaj-art-106cfe69852a4ac3b0c71fdca6c68e7c2024-12-18T08:55:18ZengKeAi Communications Co., Ltd.Nano TransMed2790-67602024-12-013100037Rapid detection of spermine by using a cost-effective carbon nanostructured aptasensorHowyn Tang0Hossein Pouri1Chao Lu2Jin Zhang3School of Biomedical Engineering, University of Western Ontario, London, Ontario N6A 5B9, CanadaDepartment of Chemical and Biochemical Engineering, University of Western Ontario, London, Ontario N6A 5B9, CanadaDepartment of Chemical and Biochemical Engineering, University of Western Ontario, London, Ontario N6A 5B9, CanadaSchool of Biomedical Engineering, University of Western Ontario, London, Ontario N6A 5B9, Canada; Department of Chemical and Biochemical Engineering, University of Western Ontario, London, Ontario N6A 5B9, Canada; Corresponding author at: Department of Chemical & Biochemical Engineering, University of Western Ontario, London, Ontario N6A 5B9, Canada.Spermine is a polyamine that participates in ion channel regulation and cell proliferation. It is considered a biomarker for pancreatic cancer. In this study, a carbon nanostructure-based aptasensor has been developed to rapidly detect spermine in solution by relying on Förster resonance energy transfer (FRET). The FRET donor, carbon quantum dots (CDs), are bioconjugated to aptamers specific for spermine. CDs have an average diameter of 5.0 ± 2.5 nm and have the maximum fluorescence intensity emitting at 510 nm when excited at 400 nm. Due to the π-π* interaction between DNA-based aptamer and GO, a FRET quencher, CDs bioconjugated with aptamer (CDs-aptamer) can directly attach on the surface of GO, resulting in significant FRET quenching. In the presence of spermine, GO and CDs-Aptamer separate, and the fluorescence intensity of CDs is restored. The restored fluorescence intensity of CD-aptamer can be observed when the concentration of spermine increases from 0.1 to 250 nM. The sensing system in this study could be a cost-effective, user-friendly method to quickly detect spermine, a biomarker for pancreatic cancer.http://www.sciencedirect.com/science/article/pii/S2790676024000086Carbon quantum dotsGraphene oxideSperminePancreatic cancerFRET |
| spellingShingle | Howyn Tang Hossein Pouri Chao Lu Jin Zhang Rapid detection of spermine by using a cost-effective carbon nanostructured aptasensor Nano TransMed Carbon quantum dots Graphene oxide Spermine Pancreatic cancer FRET |
| title | Rapid detection of spermine by using a cost-effective carbon nanostructured aptasensor |
| title_full | Rapid detection of spermine by using a cost-effective carbon nanostructured aptasensor |
| title_fullStr | Rapid detection of spermine by using a cost-effective carbon nanostructured aptasensor |
| title_full_unstemmed | Rapid detection of spermine by using a cost-effective carbon nanostructured aptasensor |
| title_short | Rapid detection of spermine by using a cost-effective carbon nanostructured aptasensor |
| title_sort | rapid detection of spermine by using a cost effective carbon nanostructured aptasensor |
| topic | Carbon quantum dots Graphene oxide Spermine Pancreatic cancer FRET |
| url | http://www.sciencedirect.com/science/article/pii/S2790676024000086 |
| work_keys_str_mv | AT howyntang rapiddetectionofsperminebyusingacosteffectivecarbonnanostructuredaptasensor AT hosseinpouri rapiddetectionofsperminebyusingacosteffectivecarbonnanostructuredaptasensor AT chaolu rapiddetectionofsperminebyusingacosteffectivecarbonnanostructuredaptasensor AT jinzhang rapiddetectionofsperminebyusingacosteffectivecarbonnanostructuredaptasensor |