Comparative Characteristics of Immunochromatographic Test Systems for Tylosin Antibiotic in Meat Products
Tylosin (TYL) is a macrolide antibiotic widely used in animal husbandry. Due to associated health risks, there is a demand for sensitive methods for mass screening of TYL in products of animal origin. This article describes the development of lateral flow immunoassays (LFIAs) for TYL detection using...
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2024-10-01
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| author | Lyubov V. Barshevskaya Dmitriy V. Sotnikov Elena A. Zvereva Boris B. Dzantiev Anatoly V. Zherdev |
| author_facet | Lyubov V. Barshevskaya Dmitriy V. Sotnikov Elena A. Zvereva Boris B. Dzantiev Anatoly V. Zherdev |
| author_sort | Lyubov V. Barshevskaya |
| collection | DOAJ |
| description | Tylosin (TYL) is a macrolide antibiotic widely used in animal husbandry. Due to associated health risks, there is a demand for sensitive methods for mass screening of TYL in products of animal origin. This article describes the development of lateral flow immunoassays (LFIAs) for TYL detection using direct (anti-TYL antibodies conjugated with nanoparticles) and indirect antibody labeling (anti-species antibodies conjugated with nanoparticles and combined with native anti-TYL antibodies). The choice of LFIA conditions, such as concentrations of hapten–protein conjugates, specific antibodies, and gold nanoparticle (GNP) conjugates with antibodies, as well as incubation time of reagents and the concentration of detergent in the sample buffer, is presented. The achieved limits of TYL detection using LFIAs with indirect labeling were 0.8 ng/mL (visual) and 0.07 ng/mL (instrumental), compared to 4 ng/mL (visual) and 0.4 ng/mL (instrumental) for the case of direct labeling. The sensitivity of the LFIA using the indirect format was up to seven times higher, allowing the determination of the target analyte at low concentrations. TYL detection in ground meat using LFIA with indirect antibody labeling ranged from 76–119%. |
| format | Article |
| id | doaj-art-1067e9bfcf824070bc2e4a76b87b6f8f |
| institution | Kabale University |
| issn | 1424-8220 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
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| series | Sensors |
| spelling | doaj-art-1067e9bfcf824070bc2e4a76b87b6f8f2024-11-08T14:41:12ZengMDPI AGSensors1424-82202024-10-012421686510.3390/s24216865Comparative Characteristics of Immunochromatographic Test Systems for Tylosin Antibiotic in Meat ProductsLyubov V. Barshevskaya0Dmitriy V. Sotnikov1Elena A. Zvereva2Boris B. Dzantiev3Anatoly V. Zherdev4A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, RussiaA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, RussiaA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, RussiaA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, RussiaA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, RussiaTylosin (TYL) is a macrolide antibiotic widely used in animal husbandry. Due to associated health risks, there is a demand for sensitive methods for mass screening of TYL in products of animal origin. This article describes the development of lateral flow immunoassays (LFIAs) for TYL detection using direct (anti-TYL antibodies conjugated with nanoparticles) and indirect antibody labeling (anti-species antibodies conjugated with nanoparticles and combined with native anti-TYL antibodies). The choice of LFIA conditions, such as concentrations of hapten–protein conjugates, specific antibodies, and gold nanoparticle (GNP) conjugates with antibodies, as well as incubation time of reagents and the concentration of detergent in the sample buffer, is presented. The achieved limits of TYL detection using LFIAs with indirect labeling were 0.8 ng/mL (visual) and 0.07 ng/mL (instrumental), compared to 4 ng/mL (visual) and 0.4 ng/mL (instrumental) for the case of direct labeling. The sensitivity of the LFIA using the indirect format was up to seven times higher, allowing the determination of the target analyte at low concentrations. TYL detection in ground meat using LFIA with indirect antibody labeling ranged from 76–119%.https://www.mdpi.com/1424-8220/24/21/6865lateral flow assayimmobilized antibodiesveterinary drugslivestockgold nanoparticles |
| spellingShingle | Lyubov V. Barshevskaya Dmitriy V. Sotnikov Elena A. Zvereva Boris B. Dzantiev Anatoly V. Zherdev Comparative Characteristics of Immunochromatographic Test Systems for Tylosin Antibiotic in Meat Products Sensors lateral flow assay immobilized antibodies veterinary drugs livestock gold nanoparticles |
| title | Comparative Characteristics of Immunochromatographic Test Systems for Tylosin Antibiotic in Meat Products |
| title_full | Comparative Characteristics of Immunochromatographic Test Systems for Tylosin Antibiotic in Meat Products |
| title_fullStr | Comparative Characteristics of Immunochromatographic Test Systems for Tylosin Antibiotic in Meat Products |
| title_full_unstemmed | Comparative Characteristics of Immunochromatographic Test Systems for Tylosin Antibiotic in Meat Products |
| title_short | Comparative Characteristics of Immunochromatographic Test Systems for Tylosin Antibiotic in Meat Products |
| title_sort | comparative characteristics of immunochromatographic test systems for tylosin antibiotic in meat products |
| topic | lateral flow assay immobilized antibodies veterinary drugs livestock gold nanoparticles |
| url | https://www.mdpi.com/1424-8220/24/21/6865 |
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